Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MP622_RS12540 Genome accession   NZ_CP094643
Coordinates   2661154..2662860 (+) Length   568 a.a.
NCBI ID   WP_016168399.1    Uniprot ID   A0A5N4WD28
Organism   Acinetobacter sp. PK01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2656154..2667860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MP622_RS12500 (MP622_12500) nusA 2656913..2658397 (-) 1485 WP_016168395.1 transcription termination factor NusA -
  MP622_RS12505 (MP622_12505) rimP 2658433..2658957 (-) 525 WP_104441190.1 ribosome maturation factor RimP -
  MP622_RS12530 (MP622_12530) secG 2659739..2660068 (-) 330 WP_016168397.1 preprotein translocase subunit SecG -
  MP622_RS12535 (MP622_12535) tpiA 2660082..2660873 (-) 792 WP_243791222.1 triose-phosphate isomerase -
  MP622_RS12540 (MP622_12540) pilB 2661154..2662860 (+) 1707 WP_016168399.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MP622_RS12545 (MP622_12545) pilC 2662889..2664115 (+) 1227 WP_243791224.1 type II secretion system F family protein Machinery gene
  MP622_RS17410 pilD 2664115..2664975 (+) 861 WP_279577664.1 A24 family peptidase Machinery gene
  MP622_RS12560 (MP622_12560) coaE 2664978..2665595 (+) 618 WP_243791225.1 dephospho-CoA kinase -
  MP622_RS12565 (MP622_12565) - 2665592..2666509 (-) 918 WP_243791227.1 DMT family transporter -
  MP622_RS12570 (MP622_12570) rlmB 2666543..2667295 (-) 753 WP_243791229.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MP622_RS12575 (MP622_12575) - 2667432..2667755 (-) 324 WP_016168405.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63617.07 Da        Isoelectric Point: 5.1024

>NTDB_id=670321 MP622_RS12540 WP_016168399.1 2661154..2662860(+) (pilB) [Acinetobacter sp. PK01]
MTALQGSPRFTGFIRRLVEDGIVSTENMQAAVNSARKTNQDIVPYLIENLKLKPIVIAEKISEEFGEPLFDLSTYDSAHI
LRDVVEDKLITKFRVLPIFRRGHQLFVATSNPTHIDAMDAMRFNSKLSIEAIIVEHDKLEKIIEQNYAEESTFEFDDDFE
LHVHEEPTDQEDNDEQPQEDEAPIVKYINKLLIDAIRMGASDLHFEPYEKVYRVRYRVDGVLRQIATPPLQLANRLASRL
KVMSQMDIAEKRVPQDGRIKLKISKSKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKALFMEALEKPQG
MLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKVPAEIPKNSLLEMGFTE
QEIQQPDFKIFQPVGCSECRDGYKGRVGIYEVMKVTPEISKIIMEDGNAIQIAEASAKLGFNNLRLSGLKKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=670321 MP622_RS12540 WP_016168399.1 2661154..2662860(+) (pilB) [Acinetobacter sp. PK01]
ATGACAGCACTACAAGGTTCTCCACGATTTACAGGTTTTATTCGCCGCTTGGTCGAAGATGGGATTGTTTCTACTGAAAA
CATGCAGGCTGCAGTTAACAGCGCAAGAAAAACAAACCAAGATATTGTCCCTTATCTAATTGAAAACCTAAAACTCAAAC
CGATTGTCATTGCCGAAAAAATTTCAGAAGAATTTGGTGAACCCTTATTTGATCTTTCGACTTATGACTCTGCCCATATT
TTAAGAGATGTCGTTGAAGACAAATTGATTACTAAATTCCGCGTGTTGCCGATTTTCCGACGTGGACATCAACTCTTTGT
AGCCACCAGCAATCCAACGCATATAGATGCCATGGATGCTATGCGCTTCAACAGCAAACTCAGTATTGAAGCGATTATTG
TTGAACATGACAAATTAGAAAAAATTATTGAACAAAATTATGCAGAAGAAAGCACTTTCGAGTTTGATGATGACTTTGAA
TTGCACGTCCATGAAGAGCCTACGGATCAAGAAGACAATGATGAGCAACCTCAAGAAGATGAAGCTCCAATTGTCAAATA
TATCAATAAATTATTGATTGATGCGATTCGTATGGGCGCATCCGACTTACATTTTGAACCCTATGAAAAAGTCTATCGTG
TGCGCTATCGTGTCGATGGGGTTTTACGCCAGATTGCAACTCCTCCTCTACAGTTAGCCAACCGTCTAGCTTCACGCTTA
AAAGTCATGTCACAAATGGACATCGCTGAAAAACGTGTGCCACAAGATGGTCGTATTAAGCTAAAAATTTCAAAATCCAA
AGCGATTGATTTTCGTGTCAACTCACTACCAACCCTATTTGGGGAAAAAATTGTACTGCGTATTTTAGACCCTTCGAGTG
CAATGCTAGGGATTGATGCACTAGGTTATGAACCTGAACAAAAAGCCTTATTTATGGAAGCACTGGAAAAGCCACAAGGG
ATGCTACTTATCACAGGCCCGACTGGTTCTGGTAAAACGGTTTCTCTCTATACAGGCTTAAATATCCTGAACAAAGAAGA
TACCAATATTTCAACCGCAGAAGATCCTGTCGAAATTAACTTAGAAGGGATTAATCAGGTTAACGTTAACAATAAAGTTG
GCTTGACGTTCTCTGCTGCATTAAAGTCTTTCTTACGTCAAGATCCAGACATTGTTATGGTCGGTGAGATTCGAGATTTA
GAAACGGCTGAAATTGCGATTAAAGCCGCGCAAACAGGTCATATGGTGATGTCTACCCTACATACCAACAGCGCACCAGA
AACATTGACTCGTTTAAGAAATATGGGCGTCCCTTCCTTTAACATTGCAACCTCTGTAAACTTAGTCATTGCGCAACGAC
TAGCTCGTCGACTCTGTTCACAATGTAAAGTACCTGCTGAAATACCAAAAAATAGCTTGTTAGAAATGGGCTTCACTGAG
CAAGAGATACAACAACCAGACTTTAAAATTTTTCAGCCTGTTGGTTGTTCAGAATGTCGAGACGGCTATAAAGGGCGTGT
GGGGATTTATGAAGTAATGAAAGTTACACCTGAAATATCCAAGATTATTATGGAAGATGGCAATGCAATTCAAATTGCAG
AAGCATCGGCAAAATTGGGGTTTAATAATCTACGTCTTTCAGGTTTAAAGAAGGTCATGCAAGGGGTAACTTCACTACAA
GAAGTCAATCGCGTGACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-151)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5N4WD28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.806
  pilB Acinetobacter baylyi ADP1

76.491

100

0.768
  pilB Legionella pneumophila strain ERS1305867

52.533

93.838

0.493
  pilF Neisseria gonorrhoeae MS11

49.643

98.592

0.489
  pilB Vibrio cholerae strain A1552

48.404

99.296

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

52.224

91.021

0.475
  pilB Vibrio campbellii strain DS40M4

51.064

91.021

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

33.981

100

0.37
  pilF Thermus thermophilus HB27

37.168

99.472

0.37