Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MTO68_RS01595 Genome accession   NZ_CP094620
Coordinates   344367..346082 (-) Length   571 a.a.
NCBI ID   WP_244014750.1    Uniprot ID   -
Organism   Acinetobacter sp. NyZ410     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339367..351082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTO68_RS01570 (MTO68_01570) rlmB 339833..340588 (+) 756 WP_004718355.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MTO68_RS01575 (MTO68_01575) - 340617..341528 (+) 912 WP_244014744.1 DMT family transporter -
  MTO68_RS01580 (MTO68_01580) coaE 341597..342196 (-) 600 WP_244014746.1 dephospho-CoA kinase -
  MTO68_RS01585 (MTO68_01585) pilD 342223..343104 (-) 882 WP_244015946.1 A24 family peptidase Machinery gene
  MTO68_RS01590 (MTO68_01590) pilC 343104..344339 (-) 1236 WP_244014748.1 type II secretion system F family protein Machinery gene
  MTO68_RS01595 (MTO68_01595) pilB 344367..346082 (-) 1716 WP_244014750.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MTO68_RS01600 (MTO68_01600) tpiA 346360..347154 (+) 795 WP_244014751.1 triose-phosphate isomerase -
  MTO68_RS01605 (MTO68_01605) secG 347164..347505 (+) 342 WP_244014753.1 preprotein translocase subunit SecG -
  MTO68_RS01630 (MTO68_01630) rimP 348297..348821 (+) 525 WP_004718339.1 ribosome maturation factor RimP -
  MTO68_RS01635 (MTO68_01635) nusA 348856..350340 (+) 1485 WP_004718337.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63566.07 Da        Isoelectric Point: 4.9143

>NTDB_id=670219 MTO68_RS01595 WP_244014750.1 344367..346082(-) (pilB) [Acinetobacter sp. NyZ410]
MSMQNGSPKFSGYIRRLVDEGFLSAQQMKDALSNAQKNKVSIVAYLSEKLNIPPLIIAEMISLEFGEPIFDLDSFNPDML
PKELIEPNLVTKHKVIPLVQRGNILYVATSDPTNIEALDAIRFNCGLNIEPIIVEHHKLEKYLEKHFTVEESFNFGDEDF
DVDIIATDDNPPSEDDDDEASEEEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLAGRLA
SRLKVMSRMDISEKRVPQDGRIKLKISKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEKQKALFMEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNLKAGLTFSVALKSFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNHCKAPVEIPRQSLLEMG
FTEQDLSNPDLQFFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKLGFDNLRRSGLIKVINGLT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=670219 MTO68_RS01595 WP_244014750.1 344367..346082(-) (pilB) [Acinetobacter sp. NyZ410]
ATGTCTATGCAAAATGGTTCACCTAAGTTTTCGGGGTATATCCGTCGCTTAGTCGACGAAGGTTTTTTATCTGCCCAGCA
AATGAAAGATGCGCTGAGTAATGCTCAAAAAAATAAAGTGAGTATTGTTGCTTATCTCAGTGAAAAACTAAATATACCGC
CACTGATCATTGCTGAAATGATCTCCCTAGAATTTGGAGAACCCATTTTTGATCTGGACAGTTTTAATCCTGACATGCTG
CCCAAGGAGCTTATTGAGCCAAATTTAGTCACTAAACATAAAGTGATTCCTTTGGTACAACGTGGCAATATTTTATATGT
CGCCACCAGTGATCCGACCAATATAGAAGCGCTGGATGCCATTCGTTTTAACTGCGGTTTGAATATTGAACCGATCATTG
TTGAACATCATAAATTAGAAAAATATTTAGAAAAGCACTTTACTGTAGAGGAAAGCTTTAATTTTGGTGATGAAGATTTT
GACGTTGATATTATTGCCACTGATGACAACCCTCCATCAGAAGATGATGACGATGAGGCTTCAGAAGAAGAAGCACCTAT
TGTTAAATATATCAATAAACTCTTGATCGATGCGATTCGTATGGGTGCATCAGATTTACACTTTGAACCTTATGAAAAAA
GTTATCGGGTGCGTTATCGTGTAGATGGGGTACTAAGAACCATTACCAATCCCCCCTTGCAATTAGCTGGCCGTTTGGCA
TCACGTCTAAAAGTGATGTCTAGAATGGATATTTCTGAAAAGCGTGTTCCGCAGGATGGCCGTATTAAGTTAAAAATATC
AAAAACCAAAGCCATTGATTTTCGTGTGAATTCACTACCGACATTATTTGGCGAAAAATTGGTACTGCGTATTCTTGACC
CTTCCAGCGCAATGTTGGGTATTGATGCGCTGGGTTATGAAGAAAAGCAAAAAGCATTATTTATGGAGGCCTTAGAAAAA
CCTCAAGGCATGTTACTGATTACTGGACCTACAGGTTCAGGTAAAACTGTTTCTCTCTATACGGGCTTAAATATCCTCAA
TACAGAAAGTTCAAATATTTCCACAGCCGAAGATCCAGTTGAAATTAACTTGGAAGGGATTAACCAAGTTAACGTCAATC
TAAAAGCAGGCCTTACCTTTTCGGTCGCACTAAAATCATTCCTGCGTCAAGATCCTGACATTATCATGGTCGGTGAGATT
CGAGACCTCGAAACTGCCGAAATTGCGATCAAGGCAGCTCAAACGGGGCATATGGTGATGTCTACACTACATACCAACAG
CGCACCTGAAACATTAACCCGTTTACGTAATATGGGTGTACCTTCATTTAATATTGCTACCTCAGTCAATCTTGTGATTG
CACAACGATTGGCAAGACGTTTATGTAATCATTGTAAAGCACCCGTTGAAATCCCTAGGCAGAGTCTGTTAGAAATGGGC
TTTACCGAACAAGACTTAAGCAATCCTGACTTACAATTTTTTCAACCTGTGGGCTGTAGTGAATGCCGTGAAGGCTATAA
AGGTCGTGTCGGGATCTACGAAGTGATGAAAGTAACACCTGAAATTTCAAAAATTATCATGGAAGATGGCAATGCGCTGC
AAATTGCGGCGGCATCTGAAAAATTGGGGTTTGATAATCTAAGACGTTCAGGTTTAATTAAGGTTATCAATGGCTTAACT
TCTTTACAAGAAATTAACCGTGTGACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.021

100

0.792
  pilB Acinetobacter baylyi ADP1

75.306

100

0.753
  pilB Legionella pneumophila strain ERS1305867

50.871

100

0.511
  pilF Neisseria gonorrhoeae MS11

50

98.774

0.494
  pilB Vibrio campbellii strain DS40M4

47.237

98.249

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

50.579

90.718

0.459
  pilB Vibrio cholerae strain A1552

50.484

90.543

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

34.584

100

0.371
  pilF Thermus thermophilus HB27

36.252

100

0.363