Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MST17_RS01385 Genome accession   NZ_CP094545
Coordinates   296469..298178 (-) Length   569 a.a.
NCBI ID   WP_180026541.1    Uniprot ID   -
Organism   Acinetobacter sp. YH16056_T     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 291469..303178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MST17_RS01355 (MST17_01355) - 291508..291831 (+) 324 WP_067763086.1 pyrimidine/purine nucleoside phosphorylase -
  MST17_RS01360 (MST17_01360) rlmB 292009..292767 (+) 759 WP_180026537.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MST17_RS01365 (MST17_01365) - 292776..293696 (+) 921 WP_373685444.1 DMT family transporter -
  MST17_RS01370 (MST17_01370) coaE 293711..294313 (-) 603 WP_180026539.1 dephospho-CoA kinase -
  MST17_RS01375 (MST17_01375) pilD 294323..295141 (-) 819 WP_180026549.1 A24 family peptidase Machinery gene
  MST17_RS01380 (MST17_01380) pilC 295207..296436 (-) 1230 WP_180026540.1 type II secretion system F family protein Machinery gene
  MST17_RS01385 (MST17_01385) pilB 296469..298178 (-) 1710 WP_180026541.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MST17_RS01390 (MST17_01390) tpiA 298454..299245 (+) 792 WP_180004673.1 triose-phosphate isomerase -
  MST17_RS01395 (MST17_01395) secG 299259..299588 (+) 330 WP_078192560.1 preprotein translocase subunit SecG -
  MST17_RS01425 (MST17_01425) rimP 300519..301043 (+) 525 WP_078189416.1 ribosome maturation factor RimP -
  MST17_RS01430 (MST17_01430) nusA 301079..302563 (+) 1485 WP_078192558.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63607.48 Da        Isoelectric Point: 4.7554

>NTDB_id=670122 MST17_RS01385 WP_180026541.1 296469..298178(-) (pilB) [Acinetobacter sp. YH16056_T]
MTAVQGSSRFTGFIRRLVDEGHLSAEKMQTALQNSKKAHQDIVPHLIQEYQLSARLIAETISIEFGEPVFDISTYDTSLI
IRDEFEDKLITKHRILPIFKHGNLLFVAISNPTNIEAVDAIRFGSKLNTETIIVEHDKLEKLIEQNFAEESTFDFDDDFD
LDLDSETPDPSQEDEDEATGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLATRLASR
LKVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFMQALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKETVEVPKQSLLELGFT
EDDLNTPEFQIYQPVGCSECREGYKGRVGIYEVMKVTPEISRIIMEDGNALQIAEASNQAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=670122 MST17_RS01385 WP_180026541.1 296469..298178(-) (pilB) [Acinetobacter sp. YH16056_T]
ATGACAGCAGTTCAAGGTTCTTCAAGATTTACGGGGTTTATTCGTCGCTTAGTTGACGAAGGGCATCTGTCTGCCGAAAA
AATGCAAACAGCGCTGCAAAATTCAAAAAAGGCTCATCAAGATATCGTGCCGCATTTGATTCAGGAATATCAACTTTCAG
CACGCCTGATTGCAGAAACCATCTCGATAGAGTTCGGTGAACCGGTTTTTGATATTTCAACTTATGACACGTCTTTAATC
ATTCGTGATGAGTTTGAAGATAAGTTAATTACCAAGCATCGTATTTTGCCCATTTTTAAACATGGCAATTTGTTATTCGT
TGCGATCAGTAATCCCACCAATATTGAAGCTGTAGATGCTATCCGATTTGGTAGTAAGCTGAATACCGAAACCATTATTG
TTGAACATGACAAACTTGAAAAGTTAATTGAACAGAACTTCGCTGAAGAAAGTACTTTTGACTTTGATGATGACTTTGAT
CTTGATTTAGATAGTGAAACACCCGATCCATCTCAAGAAGATGAAGATGAAGCAACGGGTGATGAAGCACCGATTGTTAA
GTACATCAATAAACTACTAGTCGACGCCATTCGCATGGGTGCATCCGACTTACATTTCGAACCCTATGAAAAGTCTTACC
GCGTCCGCTACCGTGTCGATGGCGTATTAAGAACAATTACCAATCCGCCCTTGCAGCTCGCTACTCGACTTGCTTCGCGT
TTAAAAGTCATGTCACAAATGGACATCTCTGAAAAACGTATACCTCAAGACGGTCGTATTAAGTTAAAGCTGTCAAAGAA
CAAAGCCATCGACTTCCGTGTGAACTCCTTACCGACCTTGTTTGGTGAAAAACTTGTTCTACGTATCCTTGACCCTTCTA
GTGCGATGTTAGGGATTGATGCGTTGGGTTATGAACCGGATCAAAAAGAACTGTTCATGCAGGCTTTAGAAAAACCGCAA
GGCATGTTGCTCATTACCGGTCCAACGGGTTCCGGTAAAACCGTATCTTTATATACAGGTCTAAACATTCTCAACCGTGA
AGATACCAACATTTCAACGGCCGAAGATCCTGTGGAAATTAACTTAGAAGGGATCAATCAGGTCAACGTAAATAATAAAG
TCGGCCTCACCTTCTCTGCAGCATTGAAGTCATTCTTACGTCAAGACCCTGATATTGTGATGGTCGGTGAGATTCGAGAT
TTAGAAACGGCTGAAATTGCCATTAAAGCAGCTCAAACGGGTCATATGGTGATGTCTACACTACATACCAATAGTGCACC
TGAAACATTGACACGCTTACGCAATATGGGCGTGCCCTCATTTAACATTGCGACATCCGTGAACCTTGTCATTGCGCAGC
GACTTGCGCGTCGCCTATGTTCACAATGTAAAGAAACAGTAGAGGTACCAAAACAAAGTCTACTGGAACTTGGTTTTACT
GAAGACGATTTAAATACACCAGAATTTCAAATATACCAACCTGTCGGTTGTTCCGAATGCCGCGAAGGCTATAAAGGTCG
TGTCGGTATATATGAAGTCATGAAAGTGACACCTGAAATTTCAAGAATTATTATGGAAGATGGCAATGCCTTACAAATTG
CCGAAGCTTCAAATCAAGCAGGTTTTAACAATTTACGCCGTTCAGGCTTAATTAAGGTCATGCAGGGGGTGACTTCACTT
CAAGAAGTCAATCGTGTGACCAGTGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.385

100

0.807
  pilB Acinetobacter baylyi ADP1

74.912

100

0.75
  pilB Legionella pneumophila strain ERS1305867

53.846

93.673

0.504
  pilF Neisseria gonorrhoeae MS11

49.11

98.77

0.485
  pilB Vibrio cholerae strain A1552

50.844

93.673

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

50.097

90.861

0.455
  pilB Vibrio campbellii strain DS40M4

49.323

90.861

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

34.084

100

0.373
  pilF Thermus thermophilus HB27

37.004

97.364

0.36