Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MST16_RS15435 Genome accession   NZ_CP094541
Coordinates   3126394..3128103 (+) Length   569 a.a.
NCBI ID   WP_180004674.1    Uniprot ID   -
Organism   Acinetobacter sp. YH16040_T     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3121394..3133103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MST16_RS15390 (MST16_15360) nusA 3122009..3123493 (-) 1485 WP_078192558.1 transcription termination factor NusA -
  MST16_RS15395 (MST16_15365) rimP 3123529..3124053 (-) 525 WP_078192559.1 ribosome maturation factor RimP -
  MST16_RS15425 (MST16_15395) secG 3124984..3125313 (-) 330 WP_078192560.1 preprotein translocase subunit SecG -
  MST16_RS15430 (MST16_15400) tpiA 3125327..3126118 (-) 792 WP_180092145.1 triose-phosphate isomerase -
  MST16_RS15435 (MST16_15405) pilB 3126394..3128103 (+) 1710 WP_180004674.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MST16_RS15440 (MST16_15410) pilC 3128136..3129362 (+) 1227 WP_168378204.1 type II secretion system F family protein Machinery gene
  MST16_RS15445 (MST16_15415) pilD 3129362..3130222 (+) 861 WP_180004675.1 A24 family peptidase Machinery gene
  MST16_RS15450 (MST16_15420) coaE 3130232..3130834 (+) 603 WP_180092148.1 dephospho-CoA kinase -
  MST16_RS15455 (MST16_15425) - 3130849..3131769 (-) 921 WP_373686350.1 DMT family transporter -
  MST16_RS15460 (MST16_15430) rlmB 3131778..3132536 (-) 759 WP_180092154.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MST16_RS15465 (MST16_15435) - 3132714..3133037 (-) 324 WP_067763086.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63688.69 Da        Isoelectric Point: 4.8334

>NTDB_id=670103 MST16_RS15435 WP_180004674.1 3126394..3128103(+) (pilB) [Acinetobacter sp. YH16040_T]
MTAVQGSSRFTGFIRRLVDEGHLSAEKMQTALQNSKKAHQDIVPHLIQEYQLSARLIAETISIEFGEPVFDISTYDTSLI
IRDEFEDKLITKHRILPIFKHGNLLFVAISNPTNIEAVDAIRFGSKLNTETIIVEHDKLEKLIEQNFAEESTFDFDDDFD
LDLEQETIDPAEQADEKDNGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKTYRVRYRVDGVLRTITNPPLQLATRLASR
LKVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFMQALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKETVEVPKQSLLELGFT
EDDLNTPEFQIYQPVGCSECREGYKGRVGIYEVMKVTPEISRIIMEDGNALQIAEASNQAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=670103 MST16_RS15435 WP_180004674.1 3126394..3128103(+) (pilB) [Acinetobacter sp. YH16040_T]
ATGACAGCAGTTCAAGGTTCTTCAAGATTTACGGGGTTTATTCGTCGCTTAGTTGACGAAGGGCATCTGTCTGCCGAAAA
AATGCAAACAGCGCTGCAAAATTCAAAAAAGGCTCATCAAGATATCGTGCCGCATTTGATTCAGGAATATCAACTTTCAG
CACGCCTGATTGCAGAAACCATCTCGATAGAGTTCGGTGAACCGGTTTTTGATATTTCAACTTATGACACGTCTTTAATC
ATTCGTGATGAGTTTGAAGATAAGTTAATTACCAAGCATCGTATTTTGCCCATTTTTAAACATGGCAATTTGTTATTCGT
TGCGATCAGTAATCCCACCAATATTGAAGCTGTAGATGCTATCCGATTTGGTAGTAAGCTCAATACCGAAACCATTATTG
TTGAACATGACAAACTTGAAAAGTTAATTGAGCAGAACTTCGCTGAAGAAAGTACTTTCGACTTTGATGATGACTTTGAT
CTTGATTTAGAACAGGAAACGATAGACCCAGCTGAACAAGCAGATGAAAAAGACAATGGTGATGAAGCGCCGATTGTTAA
ATACATCAATAAGCTGCTAGTCGATGCGATTCGTATGGGTGCATCTGACTTACATTTCGAACCCTACGAAAAGACCTATC
GCGTCCGCTACCGTGTCGATGGCGTGTTAAGAACAATTACCAATCCGCCCTTGCAGCTCGCGACTCGACTTGCTTCGCGT
TTAAAAGTCATGTCACAAATGGACATCTCGGAAAAACGTATTCCACAAGACGGTCGTATCAAGTTAAAGCTGTCAAAGAA
CAAAGCCATCGACTTCCGTGTGAACTCCTTACCGACCTTGTTTGGTGAAAAACTTGTTCTACGTATCCTTGACCCTTCTA
GTGCGATGTTAGGGATTGATGCGTTGGGTTATGAACCGGATCAAAAAGAACTGTTCATGCAGGCTTTAGAAAAACCGCAA
GGCATGTTGCTCATTACCGGTCCAACGGGTTCCGGTAAAACCGTATCTTTATATACAGGTCTAAACATTCTTAACCGTGA
AGATACCAACATTTCAACGGCCGAAGACCCTGTGGAAATTAACTTAGAAGGGATCAATCAGGTCAACGTAAATAATAAAG
TCGGCCTCACCTTCTCTGCAGCATTGAAGTCATTCTTACGTCAAGACCCTGATATTGTGATGGTCGGTGAGATTCGAGAT
TTAGAAACGGCTGAAATTGCCATTAAAGCAGCTCAAACGGGTCATATGGTGATGTCTACACTACATACCAATAGTGCACC
TGAAACATTGACACGCTTACGCAATATGGGCGTGCCCTCATTTAACATTGCGACATCCGTGAACCTTGTCATTGCGCAGC
GACTTGCGCGTCGCCTATGTTCACAATGTAAAGAAACAGTAGAGGTACCAAAACAAAGTCTACTGGAACTTGGTTTTACT
GAAGACGATTTAAATACACCAGAATTTCAAATATACCAACCTGTCGGTTGTTCCGAATGCCGCGAAGGCTATAAAGGTCG
TGTCGGTATATATGAAGTCATGAAAGTGACACCTGAAATTTCAAGAATTATTATGGAAGATGGCAATGCCTTACAAATTG
CCGAAGCTTCAAATCAAGCAGGTTTTAACAATTTACGCCGTTCAGGCTTAATTAAGGTCATGCAGGGGGTGACTTCACTT
CAAGAAGTCAATCGTGTGACCAGTGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.474

100

0.796
  pilB Acinetobacter baylyi ADP1

74.386

100

0.745
  pilB Legionella pneumophila strain ERS1305867

52.974

94.552

0.501
  pilF Neisseria gonorrhoeae MS11

49.466

98.77

0.489
  pilB Vibrio cholerae strain A1552

50.188

93.497

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.861

0.46
  pilB Vibrio campbellii strain DS40M4

49.136

91.564

0.45
  pilB/pilB1 Synechocystis sp. PCC 6803

34.032

100

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.871

97.715

0.36
  pilF Thermus thermophilus HB27

37.004

97.364

0.36