Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MRY16_RS15130 Genome accession   NZ_CP094445
Coordinates   3243009..3244184 (+) Length   391 a.a.
NCBI ID   WP_004914541.1    Uniprot ID   S7Y0P3
Organism   Acinetobacter junii strain A2-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3238009..3249184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRY16_RS15105 (MRY16_15105) - 3238673..3239146 (-) 474 WP_243483679.1 hypothetical protein -
  MRY16_RS15110 (MRY16_15110) guaD 3239332..3240657 (+) 1326 WP_243483682.1 guanine deaminase -
  MRY16_RS15115 (MRY16_15115) hpt 3240874..3241401 (+) 528 WP_004914531.1 hypoxanthine phosphoribosyltransferase -
  MRY16_RS15120 (MRY16_15120) - 3241630..3241881 (+) 252 WP_004949988.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  MRY16_RS15125 (MRY16_15125) - 3241931..3242629 (-) 699 WP_243483683.1 DsbC family protein -
  MRY16_RS15130 (MRY16_15130) pilU 3243009..3244184 (+) 1176 WP_004914541.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MRY16_RS15135 (MRY16_15135) mnmE 3244238..3245593 (-) 1356 WP_035338451.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  MRY16_RS15140 (MRY16_15140) yidC 3245684..3247441 (-) 1758 WP_099949448.1 membrane protein insertase YidC -
  MRY16_RS15145 (MRY16_15145) yidD 3247447..3247767 (-) 321 WP_004950001.1 membrane protein insertion efficiency factor YidD -
  MRY16_RS15150 (MRY16_15150) rnpA 3247777..3248172 (-) 396 WP_004950005.1 ribonuclease P protein component -
  MRY16_RS15155 (MRY16_15155) rpmH 3248201..3248335 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44323.84 Da        Isoelectric Point: 6.3220

>NTDB_id=669561 MRY16_RS15130 WP_004914541.1 3243009..3244184(+) (pilU) [Acinetobacter junii strain A2-1]
MYTTELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTKLFAYSLMNDKQRLDFENDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAIEILLNTPLIADNILKGQFHVLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSSRQDGMPVSTTSLNMVKEEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=669561 MRY16_RS15130 WP_004914541.1 3243009..3244184(+) (pilU) [Acinetobacter junii strain A2-1]
ATGTATACCACTGAATTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTCACTAAAGTAGTTGAGTATGGGGGATC
TGATTTATTTATTACCGCAGATTTCCCACCTAGCATTAAGCTACAGGGTTTGATGCGCCCTTTAGGACAACAAGCGCTTA
CCGCAGAAAAAACCAAGTTGTTTGCTTATAGTTTAATGAATGACAAACAACGTCTAGACTTCGAAAATGATTGGGAATGT
AACTTTGCCATTAATGTCCCGAATGTTTCACGATTTCGGGTCAATGTATTTAAACAACAATTACAAATAGGCATGGTGAT
TCGAACCATTACATCTGAAATTCCAACCTTTCAAAAACTAAAACTCCCAGAATCACTAAAAAATGTGATTATGGAAAAAC
GTGGTTTGGTACTGGTGGTTGGCGGAACAGGATCAGGAAAATCAACCTCTTTAGCAGCCATGATTGATCATCGAAATGAG
AACTCTGCTGGACATATCATTACCGTTGAAGATCCTGTTGAATACGTCCATAAACACAAAAAATCCATGATCACGCACCG
TGAGGTTGGAGTTGATTGTCATACATGGCATCACGCGTTGAAAAATACGCTACGCCAAGCACCAGATGTGATTTTAATTG
GTGAAATACGTGATACTGAAACGATGGAACATGCGATTGCTTTTGCTGAAACAGGGCATCTATGTTTGGGAACACTGCAC
GCCAACAATGCCAATCAGGCACTGGATCGTATTATTAACTTTTTCCCAGAGGAACGTCGCAATCAACTGTTGATGGATCT
CTCATCCAATATGAAAGCAATTATTTCTCAACGTCTAGTGCGAACTCAAGATGGACAAGGTCGTCGAGCTGCCATTGAAA
TCCTCCTCAATACGCCATTAATCGCAGACAATATTTTGAAAGGGCAATTCCATGTGCTCAAAGAGATCATGACCAAATCT
CGTGAGTTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATCTGTATAACGAAGGTGCAATTAGTTACGATGAAGCATT
GCGTAACGCCGACTCTGTCAATGAGTTACGCTTGCAAATTAAATTAAAAAGTTCACGCCAAGATGGGATGCCCGTTTCAA
CCACTTCACTGAACATGGTCAAAGAAGAGGAAGTTGAAGAGCAAGAAGAGGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7Y0P3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.197

93.606

0.545
  pilU Acinetobacter baylyi ADP1

55.62

88.747

0.494
  pilU Vibrio cholerae strain A1552

52.601

88.491

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

85.678

0.371
  pilT Acinetobacter nosocomialis M2

44.172

83.376

0.368
  pilT Acinetobacter baumannii D1279779

44.172

83.376

0.368
  pilT Acinetobacter baumannii strain A118

44.172

83.376

0.368
  pilT Acinetobacter baylyi ADP1

42.56

85.934

0.366

Multiple sequence alignment