Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MRY17_RS01980 Genome accession   NZ_CP094351
Coordinates   474160..475485 (+) Length   441 a.a.
NCBI ID   WP_243353908.1    Uniprot ID   -
Organism   Pseudomonas orientalis strain YB-76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 469160..480485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRY17_RS01960 (MRY17_01960) - 469237..471702 (-) 2466 WP_243353199.1 penicillin-binding protein 1A -
  MRY17_RS01965 (MRY17_01965) pilM 471918..472574 (+) 657 WP_243353200.1 type IV pilus assembly protein PilM -
  MRY17_RS01970 (MRY17_01970) - 472571..473122 (+) 552 WP_181282854.1 PilN domain-containing protein -
  MRY17_RS01975 (MRY17_01975) - 473119..474090 (+) 972 WP_243353201.1 pilus assembly protein PilP -
  MRY17_RS01980 (MRY17_01980) pilQ 474160..475485 (+) 1326 WP_243353908.1 type IV pilus secretin PilQ family protein Machinery gene
  MRY17_RS01985 (MRY17_01985) aroK 475490..476008 (+) 519 WP_124356688.1 shikimate kinase AroK -
  MRY17_RS01990 (MRY17_01990) aroB 476134..477234 (+) 1101 WP_191952714.1 3-dehydroquinate synthase -
  MRY17_RS01995 (MRY17_01995) - 477245..478819 (+) 1575 WP_191952715.1 AAA family ATPase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47842.84 Da        Isoelectric Point: 5.4022

>NTDB_id=669184 MRY17_RS01980 WP_243353908.1 474160..475485(+) (pilQ) [Pseudomonas orientalis strain YB-76]
MAAAVPNRVDLIHLPPPESKPLASYTGDKLNLNFQNIELRAALQQIADVAGLNLVAGDEVQGSITLRLKDVPWDQALDLV
LQAKGLDKRVKAGVLLVAPADELAARELLTLEARKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGFEGATDERGSVAV
DDRTNNIIAYQTAERLEELRRIVAQLDVPVRQVMIEARIVEANVDYDKSLGVRWGGRLHRGNWGAGGISKPLADGAQPAQ
GPPSSPFVDLGSLTGTSGLGLAFITDNLLLDLELTAMEKSGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDHVIMVVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKNGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVRAEKKSELLVFLTPRIMNNQAIAASR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=669184 MRY17_RS01980 WP_243353908.1 474160..475485(+) (pilQ) [Pseudomonas orientalis strain YB-76]
ATGGCTGCCGCTGTACCCAATCGCGTCGACCTTATCCATCTGCCGCCGCCCGAAAGTAAGCCATTGGCCAGCTATACGGG
CGACAAACTGAACCTTAATTTCCAGAACATCGAATTGCGCGCGGCATTGCAGCAAATCGCCGACGTCGCGGGCCTGAACC
TGGTGGCCGGTGACGAGGTACAGGGTTCGATCACCCTGCGGCTCAAGGACGTGCCCTGGGACCAGGCACTGGACCTGGTG
TTGCAAGCCAAGGGGCTGGACAAGCGGGTGAAGGCCGGCGTCTTGCTGGTGGCCCCCGCCGATGAATTGGCCGCACGCGA
ATTGCTGACGCTGGAGGCGCGCAAGCAGATGGCTGAGCTGGCGCCGTTACGCCGTGAGTTGCTGCAGGTCAATTACGCCA
AGGCGGCGGACCTGGCCAAGCTGTTCCAGTCCGTGACCGGCTTTGAAGGCGCGACCGACGAGCGCGGCTCGGTGGCGGTG
GATGACCGTACCAACAATATCATCGCCTACCAGACCGCAGAGCGCCTGGAAGAACTGCGGCGGATCGTGGCGCAACTGGA
CGTCCCGGTACGCCAGGTGATGATCGAGGCGCGCATTGTAGAGGCCAATGTCGATTACGACAAAAGCCTGGGGGTGCGTT
GGGGCGGCCGGCTCCATCGCGGCAATTGGGGCGCCGGGGGAATTTCCAAACCATTGGCAGACGGTGCTCAGCCCGCACAG
GGCCCGCCCAGCTCACCCTTTGTCGACCTGGGCTCGCTGACCGGCACCTCCGGCCTGGGTCTGGCCTTTATCACCGACAA
CCTGTTGCTGGACCTTGAGCTGACCGCCATGGAAAAGTCCGGCAACGGCGAAATCGTCTCACAGCCCAAGGTGGTCACCT
CGGACAAGGAAACCGCGCGCATCCTCAAGGGCACCGAGATTCCCTATCAGGAATCCACGTCCCAGGGCGCCACCTCGGTG
TCGTTCAAGGAAGCCTCGCTGTCATTGGAGGTTACACCGCAGATCACCCCCGACGATCATGTGATCATGGTGGTCAAAGT
CACCAAGGACGAGCCCGACTACCTGAACAAACTCAACGACGTTCCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTCC
TGGTCAAGAATGGCGAGACCATTGTCATCGGCGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCGGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATCATGAATAACCAGGCGATTGCTGCGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.709

99.093

0.701
  pilQ Acinetobacter baumannii strain A118

40.688

100

0.456
  pilQ Acinetobacter baumannii D1279779

40.688

100

0.456
  comQ Acinetobacter baylyi ADP1

43.077

100

0.444
  pilQ Legionella pneumophila strain Lp02

44.286

95.238

0.422
  pilQ Vibrio campbellii strain DS40M4

43.155

97.732

0.422
  pilQ Legionella pneumophila strain ERS1305867

43.81

95.238

0.417
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.89

97.279

0.417
  pilQ Vibrio cholerae strain A1552

42.89

97.279

0.417

Multiple sequence alignment