Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PM1_RS01650 Genome accession   NZ_CP094343
Coordinates   367177..369264 (+) Length   695 a.a.
NCBI ID   WP_024128058.1    Uniprot ID   -
Organism   Pseudomonas sp. M1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 362177..374264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PM1_RS01630 (MRY70_01630) pilM 364283..365347 (+) 1065 WP_009613650.1 pilus assembly protein PilM Machinery gene
  PM1_RS01635 (MRY70_01635) pilN 365347..365946 (+) 600 WP_009613648.1 type 4a pilus biogenesis protein PilN -
  PM1_RS01640 (MRY70_01640) pilO 365943..366599 (+) 657 WP_009613645.1 type 4a pilus biogenesis protein PilO -
  PM1_RS01645 (MRY70_01645) pilP 366596..367120 (+) 525 WP_009613643.1 type 4a pilus biogenesis lipoprotein PilP -
  PM1_RS01650 (MRY70_01650) pilQ 367177..369264 (+) 2088 WP_024128058.1 type IV pilus secretin PilQ Machinery gene
  PM1_RS01655 (MRY70_01655) aroK 369273..369791 (+) 519 WP_024128057.1 shikimate kinase AroK -
  PM1_RS01660 (MRY70_01660) aroB 369843..370949 (+) 1107 WP_009613636.1 3-dehydroquinate synthase -
  PM1_RS01665 (MRY70_01665) - 370956..372632 (+) 1677 WP_009613634.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74822.95 Da        Isoelectric Point: 5.2731

>NTDB_id=669083 PM1_RS01650 WP_024128058.1 367177..369264(+) (pilQ) [Pseudomonas sp. M1]
MNRTMTRLGFALLAGLFAPSLLAANLQRVDVASLPGDRVELKLGFDEPVAAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIINLTTLVPYSTRAQGNDLIILVGQGGGAPAAAASSAPSAAPAYAAAPRPAQPKPYVPVG
KAIRNIDFQRGEQGEGNVVIDLSDPSVSPDIQEQGGKIRLDFPRTQLPDSLRVRLDVKDFATPVQFVNASAAGDKASIVI
EPSGLYDYLVFQTDNRMTVSIKPMTANEAEKRKKDSFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKASDIAKLFQSVT
GTSSGPGAQPAAGTDNRGSITVDDRTNAIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEASVDYTKELGVQWGGSL
NNRGNFNITGGANSTSNTFVDLGVSTASPTSGIGIGFVTDNTTLDLQLNAMEKTGNGEVVSQPKVVTSDKETAKILKGSE
IPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMQVKVNKDEPDYQNALLGVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQTKTTSKVPFLGDVPVVGRLFKNDYVTDTKNELLVFLTPRIMNNQAIAIDR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=669083 PM1_RS01650 WP_024128058.1 367177..369264(+) (pilQ) [Pseudomonas sp. M1]
ATGAACAGAACTATGACCCGCCTGGGCTTTGCCCTGTTGGCCGGCCTTTTCGCGCCGAGCCTGCTCGCCGCCAACCTGCA
GCGCGTCGACGTGGCCTCGCTGCCGGGGGATCGCGTGGAGCTCAAGCTGGGCTTCGACGAGCCGGTGGCCGCGCCGCGTG
GCTACACCATCGAGCAGCCGGCGCGCATCGCCCTGGACCTGCCGGGCGTGCAGAACAAGCTGGGCAGCAAGAACCGCGAG
CTGGGCGTGGGCAACGCGCGCAGCCTGACCGTGGTCGAGGCCAAGGACCGCACCCGCCTGATCATCAACCTGACCACCCT
GGTGCCGTATTCCACCCGCGCCCAGGGCAACGACCTGATCATCCTGGTCGGCCAGGGTGGCGGCGCGCCCGCCGCGGCGG
CCAGCTCCGCGCCGAGCGCGGCCCCGGCCTATGCCGCGGCGCCTCGCCCGGCGCAGCCCAAGCCCTACGTGCCGGTGGGC
AAGGCGATCCGCAACATCGACTTCCAGCGCGGCGAGCAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGAGCGT
CAGCCCGGACATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCCCGCGCACCCAGCTGCCCGACAGCCTGCGCGTGC
GCCTGGACGTGAAGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCCTCGGCTGCCGGCGACAAGGCCAGCATCGTCATC
GAGCCCAGCGGCCTGTACGACTACCTGGTGTTCCAGACCGACAACCGCATGACCGTCAGCATCAAGCCGATGACCGCCAA
CGAGGCCGAGAAGCGCAAGAAGGACAGCTTCGCCTACACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GCTCGGTGCTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGACACCGTGCAGGGCAACATCACCCTG
CGCCTGCAGAACGTGCCCTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAA
CGTCCTGCTGGTGGCGCCGGCCGACGAGATCGCCGCGCGCGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAAC
TGGCGCCCCTGCGCCGCGAGCTGATCCAGGTGAACTACGCCAAGGCCTCCGACATCGCCAAGCTGTTCCAGTCCGTGACC
GGCACCAGTTCCGGCCCGGGCGCCCAGCCTGCCGCCGGGACCGACAACCGTGGCTCCATCACCGTCGACGACCGCACCAA
CGCCATCATCGCCTACCAGACCCAGGACCGCCTGGACGAGCTGCGCCGCATCGTCTCGCAGCTGGACATCCCGGTACGCC
AGGTGATGATCGAGGCGCGCATCGTCGAGGCCAGCGTCGACTACACCAAGGAGCTGGGGGTGCAGTGGGGTGGTTCGCTG
AATAATCGCGGCAACTTCAATATCACCGGTGGCGCCAACAGCACGAGCAATACCTTCGTCGATCTGGGCGTCAGCACCGC
GTCCCCGACCTCCGGCATCGGCATCGGCTTCGTCACCGACAACACCACCCTGGACCTGCAGCTCAACGCCATGGAGAAGA
CCGGCAACGGCGAAGTGGTGTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCTCGGAG
ATTCCCTACCAGGAAGCCAGCTCCAGCGGCGCCACCTCGGTGTCCTTCAAGGAGGCCGCGCTGTCCCTGGAGGTCACTCC
GCAGATCACCCCGGACAACCGCATCATCATGCAGGTCAAGGTGAACAAGGACGAGCCCGACTACCAGAACGCGCTGCTCG
GCGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGTGAAACCATCGTCATCGGCGGGGTG
TTCTCCAACACCCAGACCAAGACCACCTCCAAGGTCCCGTTCCTCGGCGACGTACCGGTCGTCGGCCGCCTGTTCAAGAA
CGACTATGTGACGGACACCAAGAACGAGCTTCTGGTTTTCCTCACTCCCCGGATCATGAATAATCAGGCCATTGCCATCG
ATCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(381-454)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(40-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.178

100

0.803
  pilQ Acinetobacter baumannii D1279779

40.699

100

0.419
  pilQ Acinetobacter baumannii strain A118

40.699

100

0.419
  comQ Acinetobacter baylyi ADP1

41.463

100

0.416
  pilQ Legionella pneumophila strain Lp02

38.946

98.273

0.383
  pilQ Legionella pneumophila strain ERS1305867

38.799

98.273

0.381

Multiple sequence alignment