Detailed information NTG0669082

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PM1_RS01630
• Genome accession: NZ_CP094343
• Coordinates: 364283..365347 (+)
• Length: 354 a.a.
• NCBI ID: WP_009613650.1
• Uniprot ID: A0A1A5D9E0
• Organism: Pseudomonas sp. M1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 359283..370347

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PM1_RS01610 (MRY70_01610)	-	359371..359793 (+)	423	WP_009613658.1	hypothetical protein	-
PM1_RS01615 (MRY70_01615)	-	359836..360606 (+)	771	WP_009613656.1	class I SAM-dependent methyltransferase	-
PM1_RS01620 (MRY70_01620)	-	360668..361159 (+)	492	WP_009613654.1	M48 family metallopeptidase	-
PM1_RS01625 (MRY70_01625)	-	361665..364097 (-)	2433	WP_009613652.1	penicillin-binding protein 1A	-
PM1_RS01630 (MRY70_01630)	pilM	364283..365347 (+)	1065	WP_009613650.1	pilus assembly protein PilM	Machinery gene
PM1_RS01635 (MRY70_01635)	pilN	365347..365946 (+)	600	WP_009613648.1	type 4a pilus biogenesis protein PilN	-
PM1_RS01640 (MRY70_01640)	pilO	365943..366599 (+)	657	WP_009613645.1	type 4a pilus biogenesis protein PilO	-
PM1_RS01645 (MRY70_01645)	pilP	366596..367120 (+)	525	WP_009613643.1	type 4a pilus biogenesis lipoprotein PilP	-
PM1_RS01650 (MRY70_01650)	pilQ	367177..369264 (+)	2088	WP_024128058.1	type IV pilus secretin PilQ	Machinery gene
PM1_RS01655 (MRY70_01655)	aroK	369273..369791 (+)	519	WP_024128057.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37773.09 Da
• Isoelectric Point: 4.4526

>NTDB_id=669082 PM1_RS01630 WP_009613650.1 364283..365347(+) (pilM) [Pseudomonas sp. M1]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNSVVEKNIAELEGVGQALSRVVAKARTGVKTAAV
AVAGSAVITKTIEMDGGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNAERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAFGLLGDQLGGNADELTVAVVDIGATMTTLSVLHHGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMVLSNKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=669082 PM1_RS01630 WP_009613650.1 364283..365347(+) (pilM) [Pseudomonas sp. M1]
GTGCTAGGGCTCTTCACTAAGAAAGCGAACACACTGCTGGGGATCGACATCAGTTCGACTTCGGTCAAACTCCTCGAGTT
GAGCCGTTCGGGCGGGCGCTACAAGGTAGAGGCCTACGCCGTAGAGCCGCTCCCGCCGAACTCGGTGGTCGAGAAGAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGCTGTCGCGGGTGGTCGCCAAGGCGCGCACCGGCGTGAAGACGGCTGCCGTG
GCGGTCGCCGGCTCGGCGGTCATCACCAAGACCATCGAGATGGACGGCGGGCTTTCCGAAGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCCGACCAGTACATTCCCTATCCCCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGCGTATCCGCGC
GCAACGCCGAACGCGTCGACGTGCTGCTGGCGGCCTGCCGCAAGGAGAACGTCGAGGTGCGCGAGGCCGCGCTGGCCCTG
GCCGGGCTCACCGCCAAGGTGGTGGACGTCGAGGCCTACGCGCTGGAGCGCGCCTTCGGCCTGCTCGGCGACCAGTTGGG
CGGCAACGCCGACGAGCTGACCGTCGCGGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCTTGCACCACGGCC
GCACCATCTATACCCGCGAGCAGCTGTTCGGCGGCCGCCAGCTCACCGAGGAGATCCAGCGCCGCTATGGCCTGTCGGTG
GAAGAAGCCGGCCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGTTGCAACCGTTCAAGGATGC
GGTGGTGCAGCAAGTTTCGCGTTCGCTGCAGTTCTTCTTCGCCGCGGGCCAGTTCAACGACGTCGACTACATCCTGCTCG
CCGGCGGCACGGCTTCCATTCCCGACCTGGACCGGCTCATCCAGCAGAAGATCGGTACCCCGACGCTGGTGGCCAACCCC
TTCGCCGACATGGTGCTGAGCAACAAGGTGAATGCCGGCGCGCTGGCCAGTGATGCGCCGGCGCTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1A5D9E0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	53.39	100	0.534
pilM	Legionella pneumophila strain ERS1305867	46.045	100	0.46