Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MOW12_RS01705 Genome accession   NZ_CP094254
Coordinates   359150..360856 (-) Length   568 a.a.
NCBI ID   WP_005181778.1    Uniprot ID   -
Organism   Acinetobacter indicus strain BDT2076     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354150..365856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOW12_RS01675 (MOW12_01675) - 354298..354621 (+) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -
  MOW12_RS01680 (MOW12_01680) rlmB 354727..355479 (+) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MOW12_RS01685 (MOW12_01685) - 355497..356417 (+) 921 WP_005181771.1 DMT family transporter -
  MOW12_RS01690 (MOW12_01690) coaE 356414..357022 (-) 609 WP_005181773.1 dephospho-CoA kinase -
  MOW12_RS01695 (MOW12_01695) pilD 357033..357893 (-) 861 WP_075175010.1 A24 family peptidase Machinery gene
  MOW12_RS01700 (MOW12_01700) pilC 357893..359119 (-) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  MOW12_RS01705 (MOW12_01705) pilB 359150..360856 (-) 1707 WP_005181778.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MOW12_RS01710 (MOW12_01710) tpiA 361145..361936 (+) 792 WP_242911898.1 triose-phosphate isomerase -
  MOW12_RS01715 (MOW12_01715) secG 361950..362279 (+) 330 WP_005181783.1 preprotein translocase subunit SecG -
  MOW12_RS01740 (MOW12_01740) rimP 363148..363672 (+) 525 WP_005181784.1 ribosome maturation factor RimP -
  MOW12_RS01745 (MOW12_01745) nusA 363705..365189 (+) 1485 WP_005181786.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63758.21 Da        Isoelectric Point: 4.9207

>NTDB_id=668378 MOW12_RS01705 WP_005181778.1 359150..360856(-) (pilB) [Acinetobacter indicus strain BDT2076]
MSVQQAMPRFTGFIRRLVEEGLVTAENMQQAIVAAKKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQKDDDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=668378 MOW12_RS01705 WP_005181778.1 359150..360856(-) (pilB) [Acinetobacter indicus strain BDT2076]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCGACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCGAAAAAAGCCGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACCATTGCTGAAAAAATTTCCTATGAATTTGGTGAACCGATTTTTGACTTGGCGGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTAGATGAAAAACTGTTCACCAAATACCGAATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
TGCTACCAGTAACCCAACCAATATCGAAGCTATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGACAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTGGAAGTTGGCGACAACGCTCCCGAAACACAAAAAGATGACGATGAGCCACAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAACAAGTTACTGGTCGATGCAATCCGTATGGGCGCCTCGGATTTACACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTTTTGCGCCAGATTGCCAATCCTCCGTTACAATTGGCCACACGCCTTGCCTCACGTTTA
AAAGTCATGTCACAAATGGACATCTCGGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCATTGCCGACCCTGTTCGGGGAAAAAATTGTACTGCGTATTCTCGATCCATCTAGTG
CCATGCTGGGGATTGATGCACTCGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTGCTGATTACCGGTCCGACCGGTTCTGGTAAAACCGTGTCTTTATATACCGGCTTAAATATCCTCAACCGCGAAGA
CACCAATATTTCTACCGCCGAAGATCCGGTGGAAATTAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGAAAGTGG
GTCTGACCTTTGCCGCAGCACTAAAATCATTCCTGCGTCAGGATCCGGATATTGTCATGGTCGGTGAGATCCGTGACCTG
GAAACTGCAGAAATTGCCATTAAAGCCGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCTGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCTTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTGTGTCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAGCTCGGCTTTACCGAA
GAAGATTTAAACCAGCCAGAGTTCCAGCTGTATCAACCGGTTGGCTGCCCGGAATGTCGTGAAGGTTATAAAGGCCGTGT
CGGGGTCTATGAAGTGATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGCAATGCCTTGCAGATTGCAG
CAGCCTCGGCAAAATCCGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.806
  pilB Acinetobacter baylyi ADP1

78.07

100

0.783
  pilB Legionella pneumophila strain ERS1305867

50.97

99.824

0.509
  pilF Neisseria gonorrhoeae MS11

51.429

98.592

0.507
  pilB Vibrio cholerae strain A1552

49.647

99.648

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.551

98.063

0.486
  pilB Vibrio campbellii strain DS40M4

50.672

91.725

0.465
  pilF Thermus thermophilus HB27

37.128

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

33.821

100

0.366

Multiple sequence alignment