Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MPZ60_RS04015 Genome accession   NZ_CP094238
Coordinates   890649..891866 (-) Length   405 a.a.
NCBI ID   WP_012837103.1    Uniprot ID   A0A096FFA0
Organism   Comamonas sp. 7D-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 885649..896866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPZ60_RS03985 (MPZ60_03985) - 886461..887396 (+) 936 WP_034357518.1 ATP-binding protein -
  MPZ60_RS03990 (MPZ60_03990) - 887409..887879 (+) 471 WP_003062186.1 NUDIX domain-containing protein -
  MPZ60_RS03995 (MPZ60_03995) - 887998..888207 (-) 210 WP_034357520.1 DNA gyrase inhibitor YacG -
  MPZ60_RS04000 (MPZ60_04000) zapD 888307..889062 (-) 756 WP_034357522.1 cell division protein ZapD -
  MPZ60_RS04005 (MPZ60_04005) coaE 889150..889776 (-) 627 WP_034357524.1 dephospho-CoA kinase -
  MPZ60_RS04010 (MPZ60_04010) - 889780..890649 (-) 870 WP_034357526.1 A24 family peptidase -
  MPZ60_RS04015 (MPZ60_04015) pilC 890649..891866 (-) 1218 WP_012837103.1 type II secretion system F family protein Machinery gene
  MPZ60_RS04020 (MPZ60_04020) pilB 891891..893633 (-) 1743 WP_003062195.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MPZ60_RS04030 (MPZ60_04030) - 893887..894816 (-) 930 WP_003062198.1 polyprenyl synthetase family protein -
  MPZ60_RS04035 (MPZ60_04035) rplU 895159..895470 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  MPZ60_RS04040 (MPZ60_04040) rpmA 895487..895744 (+) 258 WP_003058534.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44541.27 Da        Isoelectric Point: 9.6163

>NTDB_id=668235 MPZ60_RS04015 WP_012837103.1 890649..891866(-) (pilC) [Comamonas sp. 7D-2]
MASAASKGIKEFLFEWEGKDRNGKIVRGETRAGGENQIQAMLRRQGVTPSKIKKRRTRGGKKIKPKDIALFTRQLATMMK
AGVPLLQSFDIVGRGNTNPNVTKLLNDIRSDVETGTSLSAAFRKFPLYFNSLYCNLVEAGEAAGILESLLDRLATYMEKT
EAIKSKIKSALMYPTSVMIVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMGISDYFVQYWWLIFGVLGGGIYFFM
QAWKRNERVQQFMDRTILKLPIFGVLIEKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNYLYRNATDRIQSEVSTGT
SLTVAMANANIFPSMVLQMCAIGEESGAIDHMLGKAADFYESEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=668235 MPZ60_RS04015 WP_012837103.1 890649..891866(-) (pilC) [Comamonas sp. 7D-2]
ATGGCAAGCGCAGCGTCCAAGGGAATCAAGGAATTTCTCTTCGAGTGGGAAGGCAAGGACCGCAACGGCAAGATCGTTCG
CGGCGAAACTCGTGCCGGCGGAGAAAACCAGATCCAGGCCATGCTGCGCCGCCAGGGCGTGACTCCGTCCAAGATCAAGA
AGCGCAGGACCCGCGGCGGCAAGAAGATCAAGCCCAAGGACATTGCGCTGTTCACTCGCCAGCTGGCCACCATGATGAAG
GCCGGCGTGCCGCTGCTGCAGTCTTTCGACATTGTGGGTCGGGGCAACACCAACCCTAACGTCACCAAGCTGCTCAACGA
CATCCGCTCCGATGTGGAAACCGGTACCTCGCTGAGTGCGGCCTTTCGCAAGTTTCCGCTCTATTTCAACAGCCTCTACT
GCAATCTGGTGGAGGCCGGCGAGGCCGCAGGTATTCTGGAATCGTTGCTGGACCGTCTTGCCACCTATATGGAAAAGACG
GAAGCCATCAAGTCCAAGATCAAGTCGGCGCTGATGTACCCCACATCGGTCATGATCGTGGCCTTCGTCGTGGTGACCGT
GATCATGATCTTCGTGATCCCTGCCTTCAAGGAGGTCTTCACCTCCTTTGGCGCCGACCTGCCCGCGCCCACGCTGCTGG
TGATGGGCATCAGCGATTACTTTGTTCAGTACTGGTGGCTGATCTTCGGCGTGCTGGGCGGCGGCATCTATTTCTTCATG
CAGGCCTGGAAGCGCAACGAGCGCGTGCAGCAGTTCATGGACCGCACCATACTCAAGCTGCCCATCTTCGGCGTGCTGAT
CGAGAAGTCCTGCGTGGCCCGCTGGACGCGTACGCTGTCCACCATGTTTGCCGCCGGCGTGCCGCTGGTCGAGGCGCTGG
ACTCCGTGGGAGGCGCCTCGGGCAACTACCTCTACAGAAATGCCACCGACAGGATTCAGTCGGAAGTCTCCACGGGCACC
AGCCTCACGGTTGCCATGGCCAATGCCAATATCTTCCCTTCCATGGTGCTGCAGATGTGTGCCATCGGCGAGGAATCGGG
CGCCATCGACCATATGCTGGGCAAGGCAGCCGATTTCTATGAAAGCGAAGTCGACGAAATGGTGGCGGGCCTCTCCAGCC
TGATGGAGCCCATCATCATCGTCTTCCTGGGCACCTTGATCGGCGGCATCGTGGTGTCCATGTATCTGCCTATCTTCAAG
CTGGGTCAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A096FFA0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.713

99.753

0.536
  pilG Neisseria gonorrhoeae MS11

51.889

98.025

0.509
  pilG Neisseria meningitidis 44/76-A

51.637

98.025

0.506
  pilC Legionella pneumophila strain ERS1305867

50.758

97.778

0.496
  pilC Acinetobacter baylyi ADP1

49.749

98.272

0.489
  pilC Acinetobacter baumannii D1279779

48.363

98.025

0.474
  pilC Vibrio cholerae strain A1552

40.302

98.025

0.395
  pilC Vibrio campbellii strain DS40M4

38.06

99.259

0.378
  pilC Thermus thermophilus HB27

36.634

99.753

0.365

Multiple sequence alignment