Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MN200_RS01830 Genome accession   NZ_CP093968
Coordinates   381815..383527 (-) Length   570 a.a.
NCBI ID   WP_242759982.1    Uniprot ID   -
Organism   Acinetobacter sp. LUNF3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 376815..388527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN200_RS01800 (MN200_01800) - 376911..377234 (+) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -
  MN200_RS01805 (MN200_01805) rlmB 377377..378126 (+) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MN200_RS01810 (MN200_01810) - 378160..379077 (+) 918 WP_242759978.1 DMT family transporter -
  MN200_RS01815 (MN200_01815) coaE 379092..379691 (-) 600 WP_242759979.1 dephospho-CoA kinase -
  MN200_RS01820 (MN200_01820) pilD 379698..380558 (-) 861 WP_242759980.1 A24 family peptidase Machinery gene
  MN200_RS01825 (MN200_01825) pilC 380558..381784 (-) 1227 WP_242759981.1 type II secretion system F family protein Machinery gene
  MN200_RS01830 (MN200_01830) pilB 381815..383527 (-) 1713 WP_242759982.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MN200_RS01835 (MN200_01835) tpiA 383807..384598 (+) 792 WP_242759983.1 triose-phosphate isomerase -
  MN200_RS01840 (MN200_01840) secG 384612..384941 (+) 330 WP_168159357.1 preprotein translocase subunit SecG -
  MN200_RS01870 (MN200_01870) rimP 385895..386419 (+) 525 WP_004692708.1 ribosome maturation factor RimP -
  MN200_RS01875 (MN200_01875) nusA 386459..387943 (+) 1485 WP_004692706.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63606.97 Da        Isoelectric Point: 5.0712

>NTDB_id=666077 MN200_RS01830 WP_242759982.1 381815..383527(-) (pilB) [Acinetobacter sp. LUNF3]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRMLPIYRRGNILYIATSNPTNMDAIDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPNQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=666077 MN200_RS01830 WP_242759982.1 381815..383527(-) (pilB) [Acinetobacter sp. LUNF3]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAATCATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGAGAGCCTGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTCATCACCAAGCATCGTATGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCCACCAGCAATCCAACCAACATGGATGCAATCGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCTCAGCTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCTGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCAAATGACGAAGAAGATGACTCACCTCAAGGGGATGAAGCGCCGATCGT
CAAATACATTAATAAATTACTGATCGATGCGATTCGTATGGGCGCCTCAGACTTACACTTTGAGCCTTATGAAAAATCTT
ACCGTGTTCGTTACCGTGTAGATGGAGTTCTGCGTCATATTGCCAGTCCGCCATTACAACTGGCGACTCGCCTCGCCTCT
CGTTTGAAAGTCATGTCACAAATGGACATTTCTGAAAAGCGCGTCCCGCAAGATGGCCGAATTAAATTAAAGCTGTCGAA
AAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCAACCTTGTTTGGCGAAAAGCTGGTTCTGCGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAAATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGACCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATCTCAACTGCAGAAGACCCCGTTGAGATTAACTTAGAAGGCATTAATCAGGTTAACGTGAACAATA
AAGTCGGTCTTACCTTCTCTGCTGCGCTGAAGTCATTCTTACGTCAAGATCCTGATATTGTGATGGTGGGTGAGATTCGT
GACCTTGAAACAGCGGAAATCGCGATTAAAGCCGCGCAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGCAATATGGGGGTTCCATCTTTTAACATTGCGACATCGGTCAATTTGGTAATTGCAC
AACGTTTAGCACGCCGCTTATGTAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGTTTGCTCGAAATGGGCTTT
ACTTCAACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGATGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAGTTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.228

100

0.812
  pilB Acinetobacter baylyi ADP1

76.491

100

0.765
  pilB Legionella pneumophila strain ERS1305867

54.12

93.684

0.507
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

48.677

99.474

0.484
  pilB Vibrio campbellii strain DS40M4

47.849

97.895

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

34.36

100

0.372
  pilF Thermus thermophilus HB27

36.427

100

0.365