Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MNO14_RS02995 Genome accession   NZ_CP093340
Coordinates   663656..665380 (-) Length   574 a.a.
NCBI ID   WP_241945319.1    Uniprot ID   -
Organism   Luteimonas sp. S4-F44     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 658656..670380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO14_RS02975 (MNO14_02975) - 659803..660753 (+) 951 WP_241945316.1 Nudix family hydrolase -
  MNO14_RS02980 (MNO14_02980) coaE 660767..661393 (-) 627 WP_241945317.1 dephospho-CoA kinase -
  MNO14_RS02985 (MNO14_02985) pilD 661406..662269 (-) 864 WP_241945318.1 A24 family peptidase Machinery gene
  MNO14_RS02990 (MNO14_02990) pilC 662273..663433 (-) 1161 WP_241946241.1 type II secretion system F family protein Machinery gene
  MNO14_RS02995 (MNO14_02995) pilB 663656..665380 (-) 1725 WP_241945319.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MNO14_RS03000 (MNO14_03000) - 665494..665877 (-) 384 WP_241945320.1 hypothetical protein -
  MNO14_RS03005 (MNO14_03005) aqpZ 666583..667284 (+) 702 WP_241945321.1 aquaporin Z -
  MNO14_RS03010 (MNO14_03010) - 667357..668124 (-) 768 WP_241945322.1 hypothetical protein -
  MNO14_RS03015 (MNO14_03015) - 668862..668951 (-) 90 Protein_601 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62641.07 Da        Isoelectric Point: 6.3353

>NTDB_id=663799 MNO14_RS02995 WP_241945319.1 663656..665380(-) (pilB) [Luteimonas sp. S4-F44]
MSVHPGPNLVGITGIARRLVMDGALDEAAAREALDKATAARRNVAGYLREHKLVSAAQMAAALSVEFGMPLFDPAAMAPQ
ASAVKLVSEELLNKHSVLPLFQRGPRLFVGISDPTNTLALDDIKFQTNLTVEPILVDEDSIRRTLELWLSSSDSLAGALD
DAEGLDNLDVGGDEDGGRIENVVDGKDDAPIVRFVNKVLVDAIKRGASDIHFEPYETDYRVRLRVDGMLKLAAKVPVKLN
QRIAARLKVMAQLDIAEKRAPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKVVLRILDASAAKLGVDKLGYEPDQQKLFLD
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTVEDPVEIRLPGVNQVQQNERRGMTFAAALRAFLRQDPDVIM
VGEIRDLETAEIGIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNIVSSITLVIAQRLARRLCPRCKQRVVLPDHAL
LTEGFTEEQVRAGVEIYEPVGCDECTNGYRGRVGVYQVMPMSEQIQTIVLQGGNAMQIAEAAQRAGVKDLRQSALEKARQ
GLTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=663799 MNO14_RS02995 WP_241945319.1 663656..665380(-) (pilB) [Luteimonas sp. S4-F44]
ATGAGCGTCCATCCCGGCCCCAATCTCGTCGGCATCACCGGCATCGCCCGCCGCTTGGTCATGGACGGTGCGCTGGACGA
GGCGGCCGCGCGTGAGGCGCTGGACAAAGCGACGGCGGCGCGACGGAATGTCGCAGGCTACCTGCGCGAGCACAAGCTGG
TCAGCGCGGCGCAGATGGCGGCGGCCTTGTCAGTGGAGTTCGGCATGCCGCTGTTCGACCCGGCGGCGATGGCGCCGCAG
GCGAGCGCGGTCAAGCTGGTCAGCGAGGAACTGCTCAACAAGCACTCGGTGCTGCCGCTATTCCAGCGCGGCCCGCGTCT
GTTCGTGGGCATCTCCGACCCGACCAACACGCTGGCACTGGACGACATCAAGTTTCAGACCAACCTGACGGTCGAGCCGA
TCCTGGTGGACGAGGACAGCATCCGCCGGACGCTGGAACTGTGGCTATCGAGCTCGGATTCGCTGGCCGGCGCGCTGGAC
GATGCCGAGGGCCTCGACAACCTGGACGTGGGCGGCGACGAGGACGGCGGCCGGATCGAAAACGTCGTCGACGGCAAGGA
CGATGCGCCGATCGTGCGGTTCGTCAACAAGGTGCTGGTCGATGCGATCAAGCGCGGCGCCTCGGACATCCATTTCGAGC
CCTACGAGACCGACTACCGCGTGCGGCTGCGCGTGGACGGCATGCTCAAGCTGGCGGCCAAGGTGCCGGTCAAGCTCAAC
CAGCGCATCGCCGCACGCCTGAAGGTGATGGCGCAGCTCGACATCGCCGAGAAGCGCGCGCCGCAGGACGGGCGCATCAA
GTTGAACCTGTCCAAGACGCGGCAGATCGACTTCCGCGTGAGCACGCTGCCGACGCTATTCGGCGAGAAGGTGGTGCTGC
GCATTCTCGATGCCAGCGCCGCGAAGCTGGGCGTCGACAAGTTGGGCTACGAGCCTGACCAGCAGAAGCTGTTCCTCGAT
GCGATCCACAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGTTCGGGCAAGACGGTCTCGCTGTACACCGCGCT
AGGCATTCTCAACGACGAGACCCGCAACATTTCCACGGTCGAGGACCCGGTCGAGATCCGGCTGCCGGGCGTGAACCAAG
TGCAGCAGAACGAGCGCCGGGGCATGACCTTCGCCGCGGCGCTGCGGGCATTCCTGCGTCAGGACCCGGACGTGATCATG
GTCGGCGAGATCCGCGACCTGGAAACCGCGGAGATCGGCATCAAGGCCGCGCAGACCGGCCACATGGTGCTTTCGACACT
GCACACCAACGATGCGCCGCAGACGATCGCGCGCCTGATGAATATGGGTATCGCGCCGTACAACATCGTCAGCTCGATCA
CCCTGGTAATCGCCCAGCGGCTGGCGCGGCGGCTGTGCCCGCGTTGCAAGCAGCGCGTCGTGCTGCCCGATCATGCGCTG
CTCACCGAGGGCTTCACCGAGGAGCAGGTCCGCGCCGGCGTGGAGATCTACGAGCCGGTCGGCTGCGATGAATGCACCAA
CGGCTATCGCGGACGCGTGGGCGTGTACCAGGTGATGCCGATGAGCGAACAGATTCAGACGATCGTGCTCCAGGGCGGCA
ACGCGATGCAGATCGCCGAGGCCGCGCAGCGCGCCGGCGTCAAGGACCTGCGCCAATCGGCACTGGAAAAGGCCCGCCAG
GGTCTGACCAGCCTGGCCGAGATCAACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-147)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.628

98.432

0.528
  pilB Acinetobacter baumannii D1279779

52.613

100

0.526
  pilB Legionella pneumophila strain ERS1305867

49.823

98.606

0.491
  pilB Vibrio cholerae strain A1552

47.368

99.303

0.47
  pilF Neisseria gonorrhoeae MS11

46.809

98.258

0.46
  pilB Vibrio campbellii strain DS40M4

48.502

93.031

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

48.855

91.289

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

36.946

100

0.392
  pilF Thermus thermophilus HB27

38.321

95.47

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.404

93.902

0.361