Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MNO08_RS01950 Genome accession   NZ_CP093333
Coordinates   429227..430552 (+) Length   441 a.a.
NCBI ID   WP_021492322.1    Uniprot ID   U1UNN0
Organism   Pseudomonas simiae strain H4-B59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424227..435552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO08_RS01925 (MNO08_01925) - 424316..426778 (-) 2463 WP_010213762.1 penicillin-binding protein 1A -
  MNO08_RS01930 (MNO08_01930) - 426827..426973 (+) 147 WP_021492324.1 hypothetical protein -
  MNO08_RS01935 (MNO08_01935) pilM 426997..427650 (+) 654 WP_021492323.1 type IV pilus assembly protein PilM -
  MNO08_RS01940 (MNO08_01940) - 427647..428201 (+) 555 WP_010213760.1 PilN domain-containing protein -
  MNO08_RS01945 (MNO08_01945) - 428198..429157 (+) 960 WP_047544118.1 pilus assembly protein PilP -
  MNO08_RS01950 (MNO08_01950) pilQ 429227..430552 (+) 1326 WP_021492322.1 type IV pilus secretin PilQ family protein Machinery gene
  MNO08_RS01955 (MNO08_01955) aroK 430557..431075 (+) 519 WP_010213757.1 shikimate kinase AroK -
  MNO08_RS01960 (MNO08_01960) aroB 431201..432301 (+) 1101 WP_029529260.1 3-dehydroquinate synthase -
  MNO08_RS01965 (MNO08_01965) - 432312..433883 (+) 1572 WP_010213754.1 AAA family ATPase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47760.71 Da        Isoelectric Point: 4.8179

>NTDB_id=663708 MNO08_RS01950 WP_021492322.1 429227..430552(+) (pilQ) [Pseudomonas simiae strain H4-B59]
MAAAVPNRVDLIQLPPPGGTTLSAYTGDKLSLNFQSIELRTALQQIADVAGLNLVASDDVQGSITLRLKDVSWDQALDLV
LQAKGLDKRVKAGVLLVAPAEELAARELLTLESRKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGLEGVTDERGSVAV
DDRTNNIIAYQTVERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGARWGGRLNRGNWGAGGIAKPPPEGAAPPE
NPPPSPFVDLGSLTGTSGLGIAYITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSSGATSV
SFKEASLSLEVTPQITPDDRVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=663708 MNO08_RS01950 WP_021492322.1 429227..430552(+) (pilQ) [Pseudomonas simiae strain H4-B59]
ATGGCTGCTGCTGTGCCCAATCGTGTAGATCTGATCCAGTTGCCGCCTCCCGGCGGCACCACGCTGAGCGCCTACACAGG
CGACAAGCTCAGCCTCAACTTCCAGAGCATCGAGTTGCGCACGGCGTTGCAACAAATTGCCGATGTGGCGGGCCTCAATC
TGGTTGCCAGTGACGATGTGCAGGGTTCGATCACCCTGCGCCTCAAGGATGTGTCCTGGGATCAGGCGCTGGACCTGGTG
CTGCAAGCCAAGGGCCTGGACAAGCGGGTGAAGGCCGGCGTGTTGTTGGTGGCGCCGGCCGAGGAACTGGCTGCGCGCGA
GCTGCTCACGCTGGAATCGCGAAAGCAGATGGCCGAGCTGGCGCCGTTGCGCCGCGAGCTGCTGCAAGTCAATTACGCCA
AGGCGGCAGACCTGGCCAAGTTGTTCCAGTCGGTCACCGGCCTTGAGGGCGTGACCGATGAGCGTGGTTCGGTGGCGGTG
GATGATCGCACCAACAACATCATCGCCTATCAGACTGTCGAGCGACTGGAGGAACTGCGACGGATCGTGGCGCAGCTGGA
TATTCCCGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAATGTCGATTACGACAAAAGCCTGGGCGCGCGCT
GGGGTGGGCGGCTCAATCGGGGTAACTGGGGCGCGGGTGGCATTGCGAAACCTCCGCCTGAGGGCGCCGCACCGCCGGAA
AACCCACCCCCATCACCTTTTGTGGACTTGGGATCACTCACCGGCACCTCGGGCCTGGGCATTGCCTATATCACCGACAA
CCTGTTGCTGGACCTGGAGCTCACCGCCATGGAGAAAACCGGCAACGGCGAAATCGTCTCGCAACCCAAGGTGGTCACTT
CCGACAAAGAAACCGCACGCATCCTCAAGGGCACCGAGATTCCCTACCAGGAATCCAGCTCCAGCGGCGCTACGTCAGTG
TCGTTCAAGGAGGCCTCGCTGTCGTTGGAAGTCACCCCGCAGATCACCCCCGATGACCGCGTGATCATGGAGGTCAAGGT
CACCAAGGATGAGCCCGACTACTTGAACAAACTCAACGACGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCAAGGTAT
TGGTCAAGGATGGCGAGACCATCGTGATCGGCGGAGTTTTCTCCAATACCCAGAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U1UNN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.854

99.093

0.712
  comQ Acinetobacter baylyi ADP1

42.222

100

0.431
  pilQ Acinetobacter baumannii D1279779

40.733

100

0.429
  pilQ Acinetobacter baumannii strain A118

40.733

100

0.429
  pilQ Vibrio campbellii strain DS40M4

43.632

96.145

0.42
  pilQ Legionella pneumophila strain Lp02

43.81

95.238

0.417
  pilQ Vibrio cholerae strain A1552

43.541

94.785

0.413
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.541

94.785

0.413
  pilQ Legionella pneumophila strain ERS1305867

43.333

95.238

0.413