Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AS4_RS01615 Genome accession   NZ_AP014630
Coordinates   347356..349068 (-) Length   570 a.a.
NCBI ID   WP_096732752.1    Uniprot ID   -
Organism   Acinetobacter guillouiae strain NBRC 110550     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 342356..354068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS4_RS01590 (AS4_03110) rlmB 342812..343567 (+) 756 WP_096732746.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AS4_RS01595 (AS4_03120) - 343606..344517 (+) 912 WP_096732747.1 DMT family transporter -
  AS4_RS01600 (AS4_03130) coaE 344586..345185 (-) 600 WP_096732748.1 dephospho-CoA kinase -
  AS4_RS01605 (AS4_03140) pilD 345212..346093 (-) 882 WP_171478414.1 A24 family peptidase Machinery gene
  AS4_RS01610 (AS4_03150) pilC 346099..347328 (-) 1230 WP_096732750.1 type II secretion system F family protein Machinery gene
  AS4_RS01615 (AS4_03160) pilB 347356..349068 (-) 1713 WP_096732752.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AS4_RS01620 (AS4_03170) tpiA 349346..350140 (+) 795 WP_096732754.1 triose-phosphate isomerase -
  AS4_RS01625 (AS4_03180) secG 350150..350491 (+) 342 WP_034613616.1 preprotein translocase subunit SecG -
  AS4_RS01650 (AS4_03190) rimP 351283..351807 (+) 525 WP_004718339.1 ribosome maturation factor RimP -
  AS4_RS01655 (AS4_03200) nusA 351842..353326 (+) 1485 WP_004718337.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63407.87 Da        Isoelectric Point: 4.9166

>NTDB_id=66356 AS4_RS01615 WP_096732752.1 347356..349068(-) (pilB) [Acinetobacter guillouiae strain NBRC 110550]
MSMQNGSPKFSGYIRRLVDEGFLSAQQMKDALSNAQKNKVSIVAYLSEKLNIPPLIIAEMISEEFGEPIFDLDSFNPEML
PKELIDPNLVSKHKVIPLVQRGNILYVATSDPTNIEALDAIRFNCGLNIEPIIVEHHKLEKYLEKHFTVEESFNFGDEDF
DVDVIATDDTPPSEDDDEASEEEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLASRLAS
RLKVMSRMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEKQKALFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNLKAGLSFSVALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNHCKAPVEIPRQSLLEMGF
TEQDLSNPDLQIFQPIGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKLGFDNLRRSGLIKVINGLTS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=66356 AS4_RS01615 WP_096732752.1 347356..349068(-) (pilB) [Acinetobacter guillouiae strain NBRC 110550]
ATGTCTATGCAAAATGGTTCACCTAAGTTTTCGGGGTATATCCGTCGCTTAGTCGACGAAGGTTTTTTATCTGCCCAGCA
AATGAAAGATGCGCTGAGTAACGCTCAAAAAAATAAAGTGAGTATCGTTGCCTATCTCAGTGAAAAATTAAATATACCAC
CATTGATCATTGCTGAAATGATCTCCGAAGAATTTGGAGAGCCCATTTTTGATTTGGACAGCTTTAATCCTGAAATGCTG
CCCAAGGAGCTTATTGACCCAAATTTAGTTAGCAAACATAAAGTGATTCCTTTGGTTCAACGCGGCAATATTTTATACGT
TGCCACCAGTGATCCAACCAATATAGAGGCGCTAGATGCTATTCGTTTTAACTGCGGTTTAAATATTGAACCAATTATTG
TTGAACATCATAAATTAGAAAAATATTTAGAAAAGCACTTTACAGTAGAAGAAAGCTTTAATTTTGGTGATGAAGATTTT
GATGTTGATGTCATTGCCACTGATGACACACCGCCATCAGAAGATGATGACGAAGCATCTGAAGAAGAAGCACCTATTGT
TAAATATATTAATAAACTCTTGGTTGATGCAATTCGTATGGGTGCATCAGATTTACACTTTGAACCTTATGAAAAAAGTT
ATCGGGTGCGTTATCGTGTAGATGGTGTACTGAGAACCATTACCAATCCCCCCTTACAATTGGCAAGTCGTTTGGCCTCA
CGTTTAAAAGTGATGTCTAGAATGGATATTTCAGAAAAACGTGTTCCACAAGATGGTCGTATTAAGTTAAAACTCTCAAA
GACCAAAGCCATTGATTTTCGTGTGAACTCATTACCGACATTATTTGGCGAAAAATTGGTACTGCGTATTCTTGATCCTT
CCAGTGCCATGTTGGGTATTGATGCGCTAGGTTATGAGGAAAAGCAAAAAGCATTATTTATGGAGGCCTTAGAAAAACCT
CAAGGCATGTTACTGATCACAGGACCAACAGGTTCAGGTAAAACAGTTTCTCTTTATACAGGTTTAAATATCCTCAATAC
AGAAAGTTCAAACATCTCCACTGCTGAGGATCCTGTTGAAATTAACTTGGAAGGGATTAACCAAGTTAACGTCAATCTCA
AAGCAGGCCTCAGCTTTTCAGTCGCACTGAAATCATTCCTACGTCAAGATCCAGACATTATCATGGTCGGTGAGATTCGA
GACCTTGAAACTGCGGAAATTGCGATTAAAGCTGCTCAAACAGGTCATATGGTGATGTCTACCCTACATACCAACAGCGC
ACCTGAAACATTGACCCGTTTACGTAATATGGGTGTGCCTTCATTTAACATTGCCACTTCAGTCAATCTTGTGATTGCGC
AACGATTGGCAAGACGTTTATGTAATCATTGTAAAGCACCTGTTGAAATCCCTAGACAGAGTCTGTTAGAAATGGGCTTC
ACTGAACAAGATTTAAGCAACCCCGATTTACAAATTTTCCAACCCATAGGATGTAGTGAATGTCGTGAAGGCTATAAAGG
TCGTGTCGGTATCTATGAAGTGATGAAAGTAACACCTGAAATTTCAAAAATTATCATGGAAGATGGCAATGCGCTTCAAA
TTGCAGCGGCATCGGAGAAATTGGGGTTTGATAATCTAAGACGTTCGGGTTTAATTAAGGTCATCAATGGCTTAACTTCT
TTACAAGAAATTAACCGTGTGACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.159

100

0.793
  pilB Acinetobacter baylyi ADP1

75.088

100

0.751
  pilB Legionella pneumophila strain ERS1305867

50.174

100

0.505
  pilF Neisseria gonorrhoeae MS11

49.734

98.772

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

50.772

90.877

0.461
  pilB Vibrio cholerae strain A1552

50.87

90.702

0.461
  pilB Vibrio campbellii strain DS40M4

49.52

91.404

0.453
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.286

100

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

34.421

100

0.37
  pilF Thermus thermophilus HB27

36.077

100

0.361

Multiple sequence alignment