Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N023_RS03945 Genome accession   NZ_CP093321
Coordinates   891932..893149 (-) Length   405 a.a.
NCBI ID   WP_024664612.1    Uniprot ID   -
Organism   Pseudomonas syringae CC440     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 886932..898149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N023_RS03915 (N023_03915) - 887304..887771 (+) 468 WP_024664607.1 hypothetical protein -
  N023_RS03920 (N023_03920) - 888062..889534 (+) 1473 WP_024664608.1 tetratricopeptide repeat protein -
  N023_RS03925 (N023_03925) - 889641..890099 (+) 459 WP_024664609.1 hypothetical protein -
  N023_RS03930 (N023_03930) yacG 890232..890441 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  N023_RS03935 (N023_03935) coaE 890438..891061 (-) 624 WP_024664610.1 dephospho-CoA kinase -
  N023_RS03940 (N023_03940) pilD 891058..891930 (-) 873 WP_024664611.1 A24 family peptidase Machinery gene
  N023_RS03945 (N023_03945) pilC 891932..893149 (-) 1218 WP_024664612.1 type II secretion system F family protein Machinery gene
  N023_RS03950 (N023_03950) pilB 893152..894846 (-) 1695 WP_024664613.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N023_RS03955 (N023_03955) - 895070..895498 (+) 429 WP_024664614.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N023_RS03960 (N023_03960) - 895966..896487 (+) 522 WP_024664615.1 glutathione binding-like protein -
  N023_RS03965 (N023_03965) - 896729..897421 (+) 693 WP_032623610.1 DUF433 domain-containing protein -
  N023_RS03970 (N023_03970) - 897418..897825 (+) 408 WP_024664617.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44004.96 Da        Isoelectric Point: 10.1950

>NTDB_id=663526 N023_RS03945 WP_024664612.1 891932..893149(-) (pilC) [Pseudomonas syringae CC440]
MASKAIKVIVYTWEGVDKKGAKTSGELSGHNLALVKAQLRKQGINPTKVRKKSVSIFGKGKKIKPLDIAFFARQMATMMK
AGVPLLQSFDIIGEGAENPNMRALVSSLKQEVSAGNSFAMALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVAVIVSGILLIKVVPQFQSVFAGFGAELPTFTLMVIGLSEVVQKWWLAIVGLFFASVFIFK
RAYKRSQKFRDSLDRFLLKVPVIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDNMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVVVGGLVIAMYLPIFK
LGDVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=663526 N023_RS03945 WP_024664612.1 891932..893149(-) (pilC) [Pseudomonas syringae CC440]
ATGGCCAGCAAGGCAATCAAAGTCATCGTTTACACGTGGGAAGGTGTCGACAAGAAAGGCGCTAAAACCAGTGGGGAATT
GAGTGGCCATAACCTGGCCCTTGTAAAAGCCCAACTGCGCAAACAAGGCATCAACCCAACCAAGGTACGCAAAAAATCAG
TTTCGATCTTTGGCAAGGGTAAGAAGATCAAGCCTCTGGATATCGCTTTTTTCGCTCGACAGATGGCAACGATGATGAAA
GCCGGAGTTCCCTTATTACAATCGTTCGACATCATTGGTGAAGGTGCAGAAAATCCAAATATGCGAGCGCTGGTGAGTTC
ACTCAAACAGGAGGTATCGGCAGGCAACAGCTTTGCAATGGCTTTACGGCAAAAGCCTGAGTATTTCGATGATCTTTTTT
GCAATCTCGTGGATGCCGGCGAGCAAGCAGGAGCTCTGGAGAGCTTGCTGGATCGAGTAGCGAGCTACAAAGAAAAAACA
GAAAAGCTCAAAGCAAAAATAAAGAAAGCAATGACTTACCCTGCCGCGGTGTTAATCGTGGCGGTCATCGTATCTGGCAT
TCTATTAATAAAAGTAGTCCCGCAGTTTCAGTCAGTATTCGCAGGATTCGGCGCCGAGCTACCTACATTCACTTTGATGG
TCATAGGACTTTCAGAGGTAGTTCAGAAGTGGTGGCTAGCGATTGTAGGCCTGTTTTTCGCTAGCGTCTTCATATTCAAG
CGCGCGTATAAACGATCACAAAAATTTCGCGATAGTCTAGATCGTTTTTTACTTAAAGTACCTGTCATTGGGCCGCTGAT
TTTCAAATCCTCAGTGGCGCGATATGCTCGTACTCTTGCAACTACTTTTGCGGCTGGGGTTCCTCTAGTAGAGGCTCTCG
ACTCTGTTGCAGGAGCAACCGGCAATGTAGTTTTCAGAAACGCAGTTAATAAAGTCAAGCAAGATGTGTCCACAGGTATG
CAACTCAACTTCTCAATGCGATCAACCGGGGTTTTCCCGAGCTTGGCAATTCAGATGACCGCGATCGGAGAAGAGTCCGG
TGCTCTGGACAACATGTTGGACAAGGTCGCGACTTACTATGAGGATGAAGTCGACAACATGGTTGATAGCCTGACCAGCC
TAATGGAGCCCATGATCATGGCCGTCCTCGGGGTAGTAGTTGGCGGTCTAGTAATTGCGATGTACTTGCCAATATTCAAA
CTCGGGGATGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.049

100

0.76
  pilC Acinetobacter baylyi ADP1

59.559

100

0.6
  pilC Acinetobacter baumannii D1279779

59.406

99.753

0.593
  pilC Legionella pneumophila strain ERS1305867

52.13

98.519

0.514
  pilC Vibrio campbellii strain DS40M4

43.073

98.025

0.422
  pilG Neisseria gonorrhoeae MS11

43.073

98.025

0.422
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilG Neisseria meningitidis 44/76-A

42.821

98.025

0.42
  pilC Thermus thermophilus HB27

39.152

99.012

0.388