Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MOO40_RS02150 Genome accession   NZ_CP093308
Coordinates   468407..470113 (-) Length   568 a.a.
NCBI ID   WP_045525681.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain AC185     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 463407..475113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOO40_RS02120 (MOO40_02125) - 463553..464515 (+) 963 WP_101151075.1 IS1595 family transposase -
  MOO40_RS02125 (MOO40_02130) yacG 464557..464751 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  MOO40_RS02130 (MOO40_02135) zapD 464761..465483 (-) 723 WP_219313758.1 cell division protein ZapD -
  MOO40_RS02135 (MOO40_02140) coaE 465521..466135 (-) 615 WP_241871655.1 dephospho-CoA kinase -
  MOO40_RS02140 (MOO40_02145) pilD 466155..467027 (-) 873 WP_102987871.1 A24 family peptidase Machinery gene
  MOO40_RS02145 (MOO40_02150) pilC 467050..468291 (-) 1242 WP_241871656.1 type II secretion system F family protein Machinery gene
  MOO40_RS02150 (MOO40_02155) pilB 468407..470113 (-) 1707 WP_045525681.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  MOO40_RS02155 (MOO40_02160) - 470117..470581 (-) 465 WP_045788906.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MOO40_RS02160 (MOO40_02165) nadC 470904..471761 (-) 858 WP_241871657.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MOO40_RS02165 (MOO40_02170) - 471767..472237 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  MOO40_RS02170 (MOO40_02175) ampD 472384..472956 (+) 573 WP_241871659.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MOO40_RS02175 (MOO40_02180) pdhR 473232..473996 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62497.87 Da        Isoelectric Point: 5.4342

>NTDB_id=663392 MOO40_RS02150 WP_045525681.1 468407..470113(-) (pilB) [Aeromonas hydrophila strain AC185]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=663392 MOO40_RS02150 WP_045525681.1 468407..470113(-) (pilB) [Aeromonas hydrophila strain AC185]
ATGACATCTAGTCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGTTA
TCTGAGCCAAGCCAAGGCGCAGCGTAAACCCTTTGTGACCTTCCTGATCGAAAATGAAATTCTCGACAGCAAAGCATTGG
CGGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCATGTCCTGCCCATTTATACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
TCCCACCAATGTCTCTGCACTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AACTCACCACAGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGATGAATCCGAGATC
TCCGAATTGGAGGTCTCGGACGAGGGATCGCGGCTCGACGAAAGCGTCAATACGGCTGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGTGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGGTAGCAACTCCGCCAGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAACTGAAGCTGTCGCGCAA
CAAATCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCACGCCTCAATATCGAGCAGCTCGGTTTCGACGATCGCCAGAAGGCGCAATATCTGCGTGCCCTGTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTTTCGCTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACTTTCGCCAGCGCCCTGCGTTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTAGGTGAAATCCGGGAT
CTGGAAACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCCGAGGCTGAACTGCTTGAGCTGGGATTTACC
CAGCAGCAGCTGGCCGCAGGCTTGAGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.536

100

0.556
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.767

99.648

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373