Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MKP10_RS18630 Genome accession   NZ_CP093164
Coordinates   3970011..3971714 (-) Length   567 a.a.
NCBI ID   WP_241529100.1    Uniprot ID   -
Organism   Stutzerimonas zhaodongensis strain LQ01-5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3965011..3976714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKP10_RS18605 (MKP10_18605) - 3966004..3966696 (+) 693 WP_241529095.1 energy-coupling factor ABC transporter permease -
  MKP10_RS18610 (MKP10_18610) yacG 3966753..3966950 (-) 198 WP_241529096.1 DNA gyrase inhibitor YacG -
  MKP10_RS18615 (MKP10_18615) coaE 3966947..3967555 (-) 609 WP_241529097.1 dephospho-CoA kinase -
  MKP10_RS18620 (MKP10_18620) pilD 3967923..3968789 (-) 867 WP_241529098.1 prepilin peptidase Machinery gene
  MKP10_RS18625 (MKP10_18625) pilC 3968792..3970009 (-) 1218 WP_241529099.1 type II secretion system F family protein Machinery gene
  MKP10_RS18630 (MKP10_18630) pilB 3970011..3971714 (-) 1704 WP_241529100.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MKP10_RS18640 (MKP10_18640) - 3971941..3972429 (+) 489 Protein_3670 pilin -
  MKP10_RS18645 (MKP10_18645) - 3972911..3973470 (+) 560 Protein_3671 REP-associated tyrosine transposase -
  MKP10_RS18650 (MKP10_18650) cysN 3973714..3975612 (-) 1899 WP_241529101.1 sulfate adenylyltransferase subunit CysN -
  MKP10_RS18655 (MKP10_18655) cysD 3975625..3976542 (-) 918 WP_241529102.1 sulfate adenylyltransferase subunit CysD -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62069.19 Da        Isoelectric Point: 5.4630

>NTDB_id=662046 MKP10_RS18630 WP_241529100.1 3970011..3971714(-) (pilB) [Stutzerimonas zhaodongensis strain LQ01-5]
MTDNMALSGLAKQMVLTGLLDDKTAQQAQQQAARNKLSLVTYVVQNKLANGRAVAALAAEQFGVAYMDLNALDKESQLND
IVSEKLARQHRVLPLIKRGHKLFVAVSDPTNQQAVTDIQFSTGLTTEAILVEDDKLGLAIDKFFESATSGLEDLADVDLD
SVDVQTVSDDGAKEDATEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKVFRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEEQKQLYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCSSCKKEVELPREALLEEGFP
ADKIGTFKIYGPIGCENCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=662046 MKP10_RS18630 WP_241529100.1 3970011..3971714(-) (pilB) [Stutzerimonas zhaodongensis strain LQ01-5]
ATGACGGACAATATGGCCCTTTCCGGCCTAGCCAAGCAGATGGTGCTGACCGGCCTGCTCGACGACAAGACTGCACAGCA
AGCGCAACAGCAGGCTGCGCGCAACAAGCTGTCGCTGGTGACCTATGTCGTGCAGAACAAGCTCGCCAATGGTCGGGCGG
TCGCTGCACTGGCAGCGGAACAGTTCGGTGTTGCGTACATGGACCTCAACGCGCTGGATAAGGAAAGCCAGCTCAATGAC
ATCGTCTCTGAAAAGTTGGCGCGCCAGCATCGTGTATTGCCGCTAATAAAGCGCGGGCACAAGCTATTCGTGGCGGTGTC
GGATCCGACCAACCAGCAGGCTGTAACGGACATCCAATTTAGCACCGGACTGACCACTGAAGCGATCTTGGTTGAAGACG
ACAAGCTTGGGTTGGCGATTGACAAGTTCTTCGAGAGTGCAACATCTGGGCTGGAGGATTTGGCAGACGTCGATCTTGAC
AGCGTCGACGTGCAGACGGTATCGGATGATGGCGCAAAAGAAGACGCGACCGAGGCCGCCGACGACGCCCCGGTCGTTCG
CTTCGTCAACAAGATGTTGCTCGACGCCATTCGTGGCGGTTCATCCGACCTGCATTTCGAGCCGTATGAAAAAGTCTTCC
GCGTACGCCTCCGTACGGATGGCATCCTGCATGAAGTGGCTCGCCCACCGATCCAGCTTGCCCCGCGAATTTCTGCGCGC
CTGAAGGTCATGGCGGGGCTGGACATCTCCGAGCGACGCAAGCCGCAGGATGGCAGGATCAAGATGAAGCTATCCAAGAC
CAAAGCCATCGACTTCCGCGTGAACACATTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCCTCCA
GCGCGCAAATGGGTATCGACGCGCTGGGCTACGAGGAAGAGCAGAAACAGCTGTACATGAATGCCCTCAAGCAGCCGCAG
GGGATGATTCTGGTGACCGGCCCGACGGGTTCGGGCAAGACTGTATCGCTCTATACGGGCCTGAACATTCTCAACACGGT
CGACGTAAACATTTCCACTGCCGAAGACCCGGTGGAAATCAACCTAGAGGGTATCAACCAGGTGAACGTCAATCCTCGTC
AGGGCATGGACTTCTCCCAGGCGTTGCGGGCGTTTCTTCGTCAGGACCCGGACATCATCATGGTCGGCGAAATCCGCGAC
CTTGAAACTGCCGAGATCGCGATCAAGGCGGCGCAAACCGGGCACATGGTGATGTCCACCTTGCACACCAATAGCGCTGC
GGAAACCCTGACCCGTTTGCGCAACATGGGCGTAGCGGCATTCAACATCGCCACTTCGGTCAACTTGATCATCGCCCAGC
GGCTGGCGCGCAAGCTGTGCAGCAGTTGCAAGAAAGAGGTGGAGTTGCCGCGCGAAGCCCTACTAGAGGAGGGTTTCCCT
GCCGATAAGATCGGAACCTTCAAAATTTACGGCCCGATAGGCTGTGAAAATTGCAAAGGCGGATACAAAGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCACTGGCCAGGATTATCATGGAAGACGGCAACTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTCAACGATCTGCGTACATCGGCTCTTGTAAAAGCCATGCAGGGCGTAACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.907

100

0.589
  pilB Acinetobacter baylyi ADP1

58.511

99.471

0.582
  pilB Legionella pneumophila strain ERS1305867

54.737

100

0.55
  pilB Vibrio parahaemolyticus RIMD 2210633

49.206

100

0.492
  pilF Neisseria gonorrhoeae MS11

49.729

97.531

0.485
  pilB Vibrio campbellii strain DS40M4

47.018

100

0.473
  pilB Vibrio cholerae strain A1552

53.069

89.065

0.473
  pilB/pilB1 Synechocystis sp. PCC 6803

40.038

92.063

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.394

93.122

0.367
  pilF Thermus thermophilus HB27

36.637

99.647

0.365