Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MMZ71_RS25965 Genome accession   NZ_CP093022
Coordinates   5516839..5518059 (+) Length   406 a.a.
NCBI ID   WP_009315898.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5511839..5523059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ71_RS25945 (MMZ71_25950) nadC 5513394..5514242 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MMZ71_RS25955 (MMZ71_25960) pilA 5514454..5514903 (-) 450 WP_031627735.1 type 4a pilus biogenesis protein PilA Machinery gene
  MMZ71_RS25960 (MMZ71_25965) pilB 5515135..5516835 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MMZ71_RS25965 (MMZ71_25970) pilC 5516839..5518059 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  MMZ71_RS25970 (MMZ71_25975) pilD 5518063..5518935 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MMZ71_RS25975 (MMZ71_25980) coaE 5518932..5519543 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  MMZ71_RS25980 (MMZ71_25985) yacG 5519540..5519740 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  MMZ71_RS25985 (MMZ71_25990) - 5519780..5519986 (-) 207 Protein_5154 hypothetical protein -
  MMZ71_RS25990 (MMZ71_25995) - 5520092..5520781 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  MMZ71_RS25995 (MMZ71_26000) - 5520778..5521248 (-) 471 WP_023096122.1 hypothetical protein -
  MMZ71_RS26000 (MMZ71_26005) - 5521245..5521670 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  MMZ71_RS26005 (MMZ71_26010) - 5521803..5522432 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  MMZ71_RS26010 (MMZ71_26015) - 5522429..5522878 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44288.04 Da        Isoelectric Point: 9.8784

>NTDB_id=661540 MMZ71_RS25965 WP_009315898.1 5516839..5518059(+) (pilC) [Pseudomonas aeruginosa strain H08]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGAIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=661540 MMZ71_RS25965 WP_009315898.1 5516839..5518059(+) (pilC) [Pseudomonas aeruginosa strain H08]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTGAAACCCATGGACATTGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGTTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCACAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGTGTGGGGGCGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCGAAAAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCAGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGCCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baumannii D1279779

60.891

99.507

0.606
  pilC Acinetobacter baylyi ADP1

60.591

100

0.606
  pilC Legionella pneumophila strain ERS1305867

54.293

97.537

0.53
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

41.562

97.783

0.406
  pilC Thermus thermophilus HB27

37.157

98.768

0.367