Detailed information NTG0660617

Bioinformatically predicted

Overview

• Name: comFC
• Type: Machinery gene
• Locus tag: MK860_RS16730
• Genome accession: NZ_CP092829
• Coordinates: 3260922..3261608 (-)
• Length: 228 a.a.
• NCBI ID: WP_185847347.1
• Uniprot ID: -
• Organism: Bacillus pumilus strain MS32
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3255922..3266608

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MK860_RS16695 (MK860_16630)	fliW	3256055..3256489 (-)	435	WP_044139341.1	flagellar assembly protein FliW	-
MK860_RS16700 (MK860_16635)	-	3256518..3257096 (-)	579	WP_003213106.1	DUF6470 family protein	-
MK860_RS16705 (MK860_16640)	flgL	3257145..3258056 (-)	912	WP_044139340.1	flagellar hook-associated protein FlgL	-
MK860_RS16710 (MK860_16645)	flgK	3258066..3259586 (-)	1521	WP_278176757.1	flagellar hook-associated protein FlgK	-
MK860_RS16715 (MK860_16650)	-	3259602..3260084 (-)	483	WP_034660146.1	flagellar protein FlgN	-
MK860_RS16720 (MK860_16655)	flgM	3260103..3260366 (-)	264	WP_003213252.1	flagellar biosynthesis anti-sigma factor FlgM	-
MK860_RS16725 (MK860_16660)	-	3260446..3260865 (-)	420	WP_034660147.1	TIGR03826 family flagellar region protein	-
MK860_RS16730 (MK860_16665)	comFC	3260922..3261608 (-)	687	WP_185847347.1	ComF family protein	Machinery gene
MK860_RS16735 (MK860_16670)	-	3261605..3261898 (-)	294	WP_278176766.1	late competence development ComFB family protein	-
MK860_RS16740 (MK860_16675)	comFA	3261965..3263344 (-)	1380	WP_278176769.1	DEAD/DEAH box helicase	Machinery gene
MK860_RS16745 (MK860_16680)	-	3263464..3264306 (-)	843	WP_113768803.1	DegV family protein	-
MK860_RS16750 (MK860_16685)	degU	3264524..3265213 (-)	690	WP_008348273.1	two-component system response regulator DegU	Regulator
MK860_RS16755 (MK860_16690)	degS	3265278..3266453 (-)	1176	WP_003213166.1	sensor histidine kinase	Regulator

Sequence

Protein

• Length: 228 a.a.
• Molecular weight: 25914.28 Da
• Isoelectric Point: 9.6527

>NTDB_id=660617 MK860_RS16730 WP_185847347.1 3260922..3261608(-) (comFC) [Bacillus pumilus strain MS32]
MICLICEGHFVASFTWKSLLLLNDDRLCIACKHHLEKIQGPICPACGRAQSNDQLCQDCSRWKERPDSTTLLVKNRSVYA
YNDLMKDVLSRFKFRGDTALAELFKQDVQAVFKRSFSIKEPVLIPIPLSKERLKERGFNQSVILASLIGEPILQPLVKIH
QTKQSKKKKNERLDQKGMFQIKQTDAIVQRDVILIDDIYTTGATIYDAARILKEAGAKSVSSFTLIRS

Nucleotide

• Length: 687 bp

>NTDB_id=660617 MK860_RS16730 WP_185847347.1 3260922..3261608(-) (comFC) [Bacillus pumilus strain MS32]
TTGATCTGCCTCATTTGTGAGGGGCATTTTGTCGCTTCTTTTACATGGAAATCATTACTTCTATTAAATGATGATCGTCT
ATGTATAGCATGTAAGCATCACCTAGAAAAAATTCAAGGCCCGATTTGTCCAGCCTGCGGGAGAGCACAGTCGAACGATC
AATTATGTCAAGACTGTTCCAGGTGGAAAGAACGCCCTGATTCTACTACATTACTTGTGAAAAATAGGTCTGTCTATGCT
TACAACGATTTGATGAAAGATGTCCTTTCCCGCTTTAAATTTCGAGGTGATACAGCTTTAGCTGAGCTTTTTAAACAAGA
TGTCCAAGCAGTATTTAAGCGCTCTTTTTCAATTAAAGAACCTGTGCTGATCCCGATTCCTTTAAGTAAAGAAAGACTAA
AGGAAAGAGGGTTTAATCAGTCCGTCATACTTGCTTCCTTGATTGGTGAGCCGATCCTTCAGCCACTTGTGAAAATACAT
CAAACCAAACAGTCCAAGAAAAAGAAAAATGAACGTTTAGATCAAAAAGGAATGTTTCAAATCAAACAGACAGATGCGAT
CGTTCAAAGGGATGTTATCTTAATTGACGACATTTATACAACAGGGGCCACCATTTATGATGCGGCGAGAATTTTAAAAG
AAGCAGGTGCCAAAAGTGTTTCCTCTTTTACGTTGATCCGTAGTTAA

Domains

Predicted by InterproScan.

• Double zinc ribbon domain (DZR_2) (3-59)

• Phosphoribosyl transferase domain (Pribosyltran) (137-226)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFC	Bacillus subtilis subsp. subtilis str. 168	51.304	100	0.518
comFC/cflB	Streptococcus mitis NCTC 12261	39.565	100	0.399
comFC/cflB	Streptococcus mitis SK321	39.565	100	0.399
comFC/cflB	Streptococcus pneumoniae TIGR4	39.565	100	0.399
comFC/cflB	Streptococcus pneumoniae Rx1	38.865	100	0.39
comFC/cflB	Streptococcus pneumoniae D39	38.865	100	0.39
comFC/cflB	Streptococcus pneumoniae R6	38.865	100	0.39