Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MK860_RS16740 Genome accession   NZ_CP092829
Coordinates   3261965..3263344 (-) Length   459 a.a.
NCBI ID   WP_278176769.1    Uniprot ID   -
Organism   Bacillus pumilus strain MS32     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3256965..3268344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK860_RS16705 (MK860_16640) flgL 3257145..3258056 (-) 912 WP_044139340.1 flagellar hook-associated protein FlgL -
  MK860_RS16710 (MK860_16645) flgK 3258066..3259586 (-) 1521 WP_278176757.1 flagellar hook-associated protein FlgK -
  MK860_RS16715 (MK860_16650) - 3259602..3260084 (-) 483 WP_034660146.1 flagellar protein FlgN -
  MK860_RS16720 (MK860_16655) flgM 3260103..3260366 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  MK860_RS16725 (MK860_16660) - 3260446..3260865 (-) 420 WP_034660147.1 TIGR03826 family flagellar region protein -
  MK860_RS16730 (MK860_16665) comFC 3260922..3261608 (-) 687 WP_185847347.1 ComF family protein Machinery gene
  MK860_RS16735 (MK860_16670) - 3261605..3261898 (-) 294 WP_278176766.1 late competence development ComFB family protein -
  MK860_RS16740 (MK860_16675) comFA 3261965..3263344 (-) 1380 WP_278176769.1 DEAD/DEAH box helicase Machinery gene
  MK860_RS16745 (MK860_16680) - 3263464..3264306 (-) 843 WP_113768803.1 DegV family protein -
  MK860_RS16750 (MK860_16685) degU 3264524..3265213 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  MK860_RS16755 (MK860_16690) degS 3265278..3266453 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  MK860_RS16760 (MK860_16695) - 3266675..3267343 (+) 669 WP_003212936.1 YigZ family protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52435.34 Da        Isoelectric Point: 10.0540

>NTDB_id=660618 MK860_RS16740 WP_278176769.1 3261965..3263344(-) (comFA) [Bacillus pumilus strain MS32]
MNMDKAMELGQYLQSRHLLTAETRCSQSDLDWLEEKGLVIRTPSIERKANGLICCRCGVSKNRYFAHSPCDKCQKDCVYC
RSCIMMGKTTECGFLYEWTGPQMEEACHSELTWQGDLSKGQKRASEKLIEAIKHKSDLLIWAVCGAGKTEVLFHGIEFAL
HQGMSVCIATPRTDVVLELEPRLKKAFQGMKIAVLYGGSPQRFQIAPLVIATTHQLMRYKHAFDVLIIDEVDAFPYSIDE
RLQFAVLKAMRKKGVRVYLSATPSKKMIRDVSRGQLEAVKIPLRFHQQPLPVPSFQWIGHWKKKLKKNQLPPKVMNWIQK
HITKKRRVLLFVPSISTMKKVTKVLRKHHLNVEGVSADDPDRKQKVQQFRDDKYDVLVTTTILERGVTIPNVQVGVLGAE
STIFTESALVQISGRAGRHPDFFKGDVFFFHFGLTRSMKQAKKHIVKMNDTAEKEFSEK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=660618 MK860_RS16740 WP_278176769.1 3261965..3263344(-) (comFA) [Bacillus pumilus strain MS32]
TTGAACATGGACAAAGCAATGGAACTAGGGCAGTATCTGCAGTCACGCCACCTGCTTACTGCTGAAACAAGATGCTCACA
GTCTGATTTGGATTGGTTGGAGGAGAAAGGGTTAGTTATCCGAACACCTTCTATTGAGAGGAAAGCAAATGGTCTAATTT
GCTGCAGATGTGGTGTATCAAAAAATCGGTACTTTGCCCACTCTCCGTGTGACAAGTGTCAAAAAGATTGTGTGTATTGT
AGATCATGCATCATGATGGGGAAAACAACGGAATGTGGCTTTCTTTATGAATGGACGGGTCCACAAATGGAAGAAGCATG
TCACTCGGAATTAACATGGCAGGGAGACCTGTCTAAAGGACAAAAAAGAGCGTCAGAAAAATTGATCGAAGCGATAAAAC
ACAAATCTGATCTACTCATCTGGGCAGTTTGCGGGGCAGGGAAAACAGAGGTGCTTTTTCACGGAATAGAATTTGCCTTA
CATCAAGGAATGAGTGTCTGCATTGCGACACCTAGAACTGATGTTGTCCTTGAACTAGAGCCGCGGCTTAAAAAAGCTTT
TCAAGGAATGAAAATAGCTGTTCTTTATGGAGGAAGTCCTCAAAGGTTCCAAATAGCACCGTTAGTAATCGCTACAACTC
ACCAGTTGATGAGATATAAACATGCATTTGATGTGCTCATTATAGATGAAGTCGACGCCTTCCCTTATTCAATCGATGAA
CGTCTCCAATTTGCTGTTTTAAAGGCAATGAGAAAGAAAGGGGTTAGGGTTTATTTAAGTGCGACGCCGTCTAAGAAAAT
GATAAGAGATGTATCTCGTGGACAACTAGAAGCCGTAAAAATTCCTCTGCGCTTTCACCAACAACCTTTACCGGTCCCAT
CCTTTCAATGGATTGGACATTGGAAGAAGAAATTAAAAAAGAATCAGCTCCCACCTAAAGTAATGAATTGGATCCAGAAA
CATATTACAAAAAAGAGAAGGGTTTTACTTTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACGAAGGTTCTTAGAAA
GCACCACTTAAATGTAGAAGGGGTGTCTGCTGATGATCCGGACAGGAAACAAAAGGTCCAGCAATTCAGAGATGACAAAT
ACGATGTACTAGTTACAACCACAATTCTAGAAAGAGGCGTAACCATTCCAAATGTGCAAGTGGGGGTACTAGGTGCAGAA
TCTACTATCTTTACAGAAAGTGCACTTGTTCAGATTTCTGGTAGAGCAGGTAGACATCCCGATTTTTTTAAAGGAGATGT
TTTCTTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATTGTAAAAATGAATGATACAGCTGAAA
AAGAGTTCTCCGAAAAATAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(320-419)

Type III restriction enzyme, res subunit (ResIII)

(117-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

54.945

99.129

0.545