Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BRPE67_RS11865 Genome accession   NZ_AP014576
Coordinates   2536749..2537966 (+) Length   405 a.a.
NCBI ID   WP_045451075.1    Uniprot ID   -
Organism   Caballeronia cordobensis strain RPE67     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2531749..2542966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRPE67_RS11835 (BRPE67_ACDS23380) cgtA 2532011..2533108 (-) 1098 WP_014192313.1 Obg family GTPase CgtA -
  BRPE67_RS11840 (BRPE67_ACDS23390) rpmA 2533252..2533512 (-) 261 WP_014192314.1 50S ribosomal protein L27 -
  BRPE67_RS11845 (BRPE67_ACDS23400) rplU 2533537..2533848 (-) 312 WP_014192315.1 50S ribosomal protein L21 -
  BRPE67_RS11850 (BRPE67_ACDS23410) - 2534121..2535113 (+) 993 WP_014192316.1 polyprenyl synthetase family protein -
  BRPE67_RS11860 (BRPE67_ACDS23420) - 2535385..2536674 (+) 1290 WP_014192317.1 HlyC/CorC family transporter -
  BRPE67_RS11865 (BRPE67_ACDS23430) pilB 2536749..2537966 (+) 1218 WP_045451075.1 GspE/PulE family protein Machinery gene
  BRPE67_RS11870 (BRPE67_ACDS23440) - 2537963..2539186 (+) 1224 WP_045451077.1 type II secretion system F family protein -
  BRPE67_RS11875 (BRPE67_ACDS23450) - 2539218..2540138 (+) 921 WP_045451079.1 prepilin peptidase -
  BRPE67_RS11880 (BRPE67_ACDS23460) coaE 2540152..2540757 (+) 606 WP_045451081.1 dephospho-CoA kinase -
  BRPE67_RS11885 (BRPE67_ACDS23470) zapD 2540926..2541681 (+) 756 WP_045452205.1 cell division protein ZapD -
  BRPE67_RS11890 (BRPE67_ACDS23480) yacG 2541754..2541945 (+) 192 WP_014192323.1 DNA gyrase inhibitor YacG -
  BRPE67_RS11895 (BRPE67_ACDS23490) - 2541955..2542386 (-) 432 WP_045451083.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43728.11 Da        Isoelectric Point: 7.5116

>NTDB_id=66039 BRPE67_RS11865 WP_045451075.1 2536749..2537966(+) (pilB) [Caballeronia cordobensis strain RPE67]
MISPSRDAFDTFTRPARPPGATAEAVSEHDTPAVSLLAETLREAAERGASDIHVEPAEHDWRIRLRVDGALHVLRRPPPH
LRDAFVTRIKVLSRMDIAERRVPQDGRLRLSLPGGRTGDYRVNSLPTLFGEKLVLRRLDTLPPDLSLGALGLDDAQSRTV
ESAIRAPHGLVLVTGPTGSGKTLSLYCFLQMLNAEARNVCSVEDPVEIQLAGINQVSVREKAGLTFAMALRAFMRQDPDV
IMVGEIRDAQTADVAVKAAQTGHLVLSTLHTNDAPAAIARLIDIGVAPYNLASALRLVTAQRLVRKLCEVCRAPSALPLA
DGFHPFEPRGCPACHGIGYRGRAGVHQVMPVSDDIRELIVSRAATHAIARTAQTQGMPALRDAAFTRVREGVTSVAEAAD
ATEIE

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=66039 BRPE67_RS11865 WP_045451075.1 2536749..2537966(+) (pilB) [Caballeronia cordobensis strain RPE67]
ATGATCTCCCCTTCCCGCGACGCCTTCGATACTTTCACGCGCCCCGCTCGCCCGCCCGGCGCTACGGCGGAAGCCGTGTC
CGAACACGATACGCCCGCCGTCTCGCTGCTCGCCGAAACGCTGCGCGAAGCCGCTGAGCGCGGCGCATCCGACATTCACG
TCGAACCGGCCGAACACGACTGGCGCATCCGGCTGCGCGTCGATGGCGCATTGCATGTGCTCAGGCGCCCGCCGCCGCAC
TTGCGCGACGCGTTCGTCACGCGCATCAAGGTGCTGTCGCGCATGGATATCGCGGAGCGGCGCGTGCCGCAGGACGGCCG
GCTGCGTCTGAGTCTGCCGGGCGGGCGCACCGGCGACTATCGCGTCAATTCGTTGCCGACGCTCTTCGGCGAAAAGCTCG
TCCTGCGCCGGCTCGACACACTGCCGCCAGACCTGTCGCTCGGCGCGCTCGGCCTCGATGACGCGCAAAGCCGCACGGTC
GAATCGGCGATTCGCGCGCCGCACGGTCTGGTGCTCGTGACAGGTCCGACCGGCAGCGGCAAGACCCTGTCGCTCTACTG
CTTCCTGCAAATGCTCAACGCCGAAGCGCGCAACGTGTGCTCGGTCGAAGATCCGGTCGAAATCCAGCTCGCGGGCATCA
ATCAGGTGAGCGTGCGTGAAAAGGCCGGGCTGACGTTCGCCATGGCGCTGCGCGCGTTCATGCGCCAGGACCCGGACGTC
ATCATGGTCGGCGAGATCCGCGACGCCCAGACCGCCGACGTCGCGGTGAAGGCCGCGCAGACGGGACACCTCGTGCTTTC
GACCCTGCACACCAACGATGCGCCCGCCGCGATCGCACGTCTCATCGACATCGGCGTGGCGCCTTATAACCTCGCCTCCG
CGCTGCGGCTGGTCACGGCGCAGCGGCTCGTGCGCAAGCTGTGCGAGGTTTGCCGCGCGCCCTCCGCGCTGCCGCTCGCC
GACGGTTTTCATCCGTTCGAGCCGCGCGGTTGTCCGGCGTGTCACGGCATTGGGTATCGCGGACGTGCCGGGGTGCATCA
GGTGATGCCGGTTTCGGATGACATACGCGAGCTGATCGTGTCGCGCGCGGCGACTCATGCGATCGCGCGCACGGCGCAGA
CGCAAGGCATGCCCGCGTTGCGCGATGCGGCCTTCACACGCGTGCGCGAGGGCGTGACCAGCGTCGCCGAAGCCGCGGAT
GCCACGGAGATCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(40-306)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

48.387

99.506

0.481
  pilF Neisseria gonorrhoeae MS11

48.718

96.296

0.469
  pilB Acinetobacter baumannii D1279779

48.346

97.037

0.469
  pilB Acinetobacter baylyi ADP1

49.091

95.062

0.467
  pilB Vibrio campbellii strain DS40M4

47.781

94.568

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

47.258

94.568

0.447
  pilB Legionella pneumophila strain ERS1305867

45.431

97.284

0.442
  pilB/pilB1 Synechocystis sp. PCC 6803

44.03

99.259

0.437
  pilB Haemophilus influenzae Rd KW20

41.253

94.568

0.39
  pilF Thermus thermophilus HB27

41.316

93.827

0.388
  pilB Haemophilus influenzae 86-028NP

40.731

94.568

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.896

95.309

0.38
  pilB Glaesserella parasuis strain SC1401

41.734

91.111

0.38

Multiple sequence alignment