Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AVFI_RS11490 Genome accession   NZ_CP092712
Coordinates   2510071..2511759 (+) Length   562 a.a.
NCBI ID   WP_188863586.1    Uniprot ID   -
Organism   Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2505071..2516759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVFI_RS11470 (AVFI_11470) pdhR 2506711..2507481 (-) 771 WP_005420885.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AVFI_RS11475 (AVFI_11475) ampD 2507768..2508319 (-) 552 WP_155663039.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AVFI_RS11480 (AVFI_11480) nadC 2508433..2509320 (+) 888 WP_012533422.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AVFI_RS11485 (AVFI_11485) pilA 2509664..2510068 (+) 405 WP_054775673.1 pilin Machinery gene
  AVFI_RS11490 (AVFI_11490) pilB 2510071..2511759 (+) 1689 WP_188863586.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AVFI_RS11495 (AVFI_11495) pilC 2511779..2513017 (+) 1239 WP_188863587.1 type II secretion system F family protein Machinery gene
  AVFI_RS11500 (AVFI_11500) pilD 2513122..2514009 (+) 888 WP_017019492.1 A24 family peptidase Machinery gene
  AVFI_RS11505 (AVFI_11505) coaE 2514006..2514629 (+) 624 WP_188863588.1 dephospho-CoA kinase -
  AVFI_RS11510 (AVFI_11510) zapD 2514635..2515381 (+) 747 WP_005420900.1 cell division protein ZapD -
  AVFI_RS11515 (AVFI_11515) yacG 2515389..2515598 (+) 210 WP_012534335.1 DNA gyrase inhibitor YacG -
  AVFI_RS11520 (AVFI_11520) mutT 2515669..2516070 (-) 402 WP_012533258.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62172.76 Da        Isoelectric Point: 5.2761

>NTDB_id=659791 AVFI_RS11490 WP_188863586.1 2510071..2511759(+) (pilB) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
MHTNLASILRQAELISATQESSIIESVQAETHTVPSALLAQGIFTPDELVSHLAQIFGLEVINVTQFDYAATCQKLGLRD
LITRYQALPVNHDSCTIRLAVADPSIAQIEDDFRFTSGKQIELVLADQGQIQAAIRRVYGAGLSANDNQHKEITQDELAS
LVDVSLDEISATEDLSQDDAPVTRFINQILLEAVRKGVSDIHFEPYEDNYRVRFRCDGILVENSRPSAHLSRRLSARLKI
MAKLDIAERRLPQDGRIKLKLNSTTSIDMRVSSLPTLWGEKIVLRLLDSSAANLNIDKLGYSDQQKALYLEALKRPQGMI
LMTGPTGSGKTVSLYSGLNILNTTEQNISTAEDPVEINLNGINQVQVNPKIGLTFAHALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVVRLGNMGIENFNLASSLSLIIAQRLARRLCNLCKQEDHLSDELREEYNIAPSA
IIYQANKEGCNECTHGYSGRIGIYEVMPFTRELSVAILKGATVRQIEQIAIQQGMDNLKTSGIKRLEDGTTSLQELNRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=659791 AVFI_RS11490 WP_188863586.1 2510071..2511759(+) (pilB) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
ATGCACACCAACCTCGCCTCTATCTTGCGTCAAGCTGAGTTAATCAGTGCGACGCAAGAAAGCAGTATCATAGAGTCAGT
TCAAGCAGAAACGCACACCGTGCCTTCTGCTCTTTTAGCTCAGGGGATATTTACTCCTGATGAACTCGTGAGTCACCTTG
CACAAATATTTGGGCTTGAGGTGATCAACGTGACTCAATTCGATTATGCTGCCACTTGTCAAAAACTAGGGCTTCGCGAT
CTCATTACTCGCTATCAAGCCCTTCCAGTTAATCACGACAGTTGCACGATTCGTCTTGCTGTCGCTGATCCAAGTATTGC
TCAAATAGAAGATGACTTTCGCTTTACCAGCGGAAAACAAATTGAATTAGTACTTGCGGATCAAGGACAAATCCAAGCCG
CTATTCGACGAGTCTACGGTGCAGGATTGTCTGCAAATGACAATCAACATAAAGAGATAACCCAAGATGAGTTAGCCTCA
CTGGTTGATGTTTCACTTGATGAGATAAGCGCTACAGAAGATCTCAGCCAAGACGATGCTCCAGTCACTCGCTTTATTAA
CCAAATTTTATTAGAAGCGGTGCGTAAAGGAGTATCAGATATTCACTTTGAACCCTATGAAGATAATTATCGAGTGCGTT
TTCGCTGCGATGGGATCTTAGTGGAAAACAGCCGCCCATCGGCTCATTTAAGCCGTCGTTTATCTGCTCGCCTTAAAATC
ATGGCAAAATTAGACATTGCAGAACGTCGTCTACCACAAGATGGGCGTATTAAACTCAAATTAAACAGTACCACTTCGAT
TGATATGCGTGTTTCTTCACTGCCAACCTTATGGGGTGAAAAAATTGTTCTGCGTTTATTAGACAGCAGCGCGGCTAATT
TAAATATTGATAAACTAGGCTATAGCGATCAACAAAAAGCCCTATATTTAGAAGCCTTAAAACGCCCTCAAGGGATGATA
TTAATGACAGGTCCTACAGGCAGTGGGAAAACCGTCTCTCTTTATTCTGGTCTTAATATTCTCAACACTACAGAACAAAA
CATTTCAACAGCGGAAGATCCAGTTGAGATCAACTTGAATGGGATAAACCAAGTTCAAGTGAATCCAAAAATCGGCCTTA
CTTTCGCTCACGCTTTACGATCTTTTTTACGTCAAGATCCAGATATCGTCATGGTAGGTGAAATACGAGATCTCGAAACG
GCAGAGATTGCGGTCAAGGCTGCTCAAACTGGGCACTTAGTTCTTTCTACCCTACATACAAATTCCGCAGCAGAAACCGT
CGTTAGGCTAGGCAACATGGGAATTGAAAATTTCAACTTAGCTTCCTCTCTTAGTTTGATCATTGCCCAACGGTTGGCTC
GTCGTCTCTGTAATTTATGCAAACAAGAAGATCATTTAAGTGATGAGTTACGAGAAGAATATAACATTGCACCTTCAGCC
ATTATTTATCAAGCCAATAAAGAAGGGTGTAATGAATGCACTCATGGTTACTCAGGGCGTATTGGTATTTATGAAGTGAT
GCCATTCACCCGAGAACTGTCTGTTGCCATTTTAAAAGGTGCAACCGTTAGACAAATAGAACAAATTGCCATTCAGCAAG
GTATGGATAACCTAAAAACCTCAGGAATTAAACGATTAGAAGATGGCACAACCAGCTTACAAGAACTAAACCGTGTACTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

68.327

100

0.683
  pilB Vibrio parahaemolyticus RIMD 2210633

68.149

100

0.681
  pilB Vibrio campbellii strain DS40M4

67.438

100

0.674
  pilB Acinetobacter baumannii D1279779

48.043

100

0.48
  pilB Legionella pneumophila strain ERS1305867

46.892

100

0.47
  pilB Acinetobacter baylyi ADP1

50.098

90.925

0.456
  pilF Neisseria gonorrhoeae MS11

44.071

100

0.443
  pilB/pilB1 Synechocystis sp. PCC 6803

38.667

93.416

0.361