Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AMJAP_RS01300 Genome accession   NZ_AP014545
Coordinates   276089..277255 (+) Length   388 a.a.
NCBI ID   WP_019620984.1    Uniprot ID   -
Organism   Amphritea japonica ATCC BAA-1530     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 271089..282255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMJAP_RS01275 (AMJAP_0240) - 272159..272452 (-) 294 WP_019620989.1 DUF167 domain-containing protein -
  AMJAP_RS01280 (AMJAP_0241) - 272459..273016 (-) 558 WP_019620988.1 YggT family protein -
  AMJAP_RS01285 (AMJAP_0242) proC 273132..273956 (-) 825 WP_019620987.1 pyrroline-5-carboxylate reductase -
  AMJAP_RS01290 (AMJAP_0243) - 274014..274712 (-) 699 WP_019620986.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMJAP_RS01295 (AMJAP_0244) pilT 275039..276073 (+) 1035 WP_019620985.1 type IV pilus twitching motility protein PilT Machinery gene
  AMJAP_RS01300 (AMJAP_0245) pilU 276089..277255 (+) 1167 WP_019620984.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMJAP_RS01305 (AMJAP_0246) - 277326..278609 (-) 1284 WP_019620983.1 dihydroorotase -
  AMJAP_RS01310 (AMJAP_0247) - 278614..279615 (-) 1002 WP_019620982.1 aspartate carbamoyltransferase catalytic subunit -
  AMJAP_RS01315 (AMJAP_0248) pyrR 279636..280151 (-) 516 WP_019620981.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AMJAP_RS01320 (AMJAP_0249) ruvX 280132..280608 (-) 477 WP_019620980.1 Holliday junction resolvase RuvX -
  AMJAP_RS01325 (AMJAP_0250) - 280647..281231 (-) 585 WP_051088395.1 YqgE/AlgH family protein -
  AMJAP_RS01330 (AMJAP_0251) - 281281..282231 (-) 951 WP_019620978.1 energy transducer TonB -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43118.13 Da        Isoelectric Point: 5.1576

>NTDB_id=65850 AMJAP_RS01300 WP_019620984.1 276089..277255(+) (pilU) [Amphritea japonica ATCC BAA-1530]
MDITQLLKVMTDRDASDLFITAGARPSIKIDGTIKPLTKESLKPAQARSLTYSTMNDKQLSEFEGTRECNFAISAQGIGR
FRVSAFFQRNSPGMVLRRINTYIPSLEELNLPPVLKDLAMTKRGLILFVGGTSTGKSTSLASMIDYRNTNSAGHIITIED
PIEYIHEHKQSVITQREVGLDTESFEIALKNTLRQAPDVILMGEIRTADTMGYGLTFAETGHLCMATLHANNANQALDRI
ISFFPPEHHAQIWMDLSLNLKAIVAQQLLPTKDGKGRRAVVEVLINTPLMQDLIRKGEVHELKEVIKKSTNLGMQTFDQA
LYNEYKEGNINYDVAIAHADSPNDLRLMIKLNADTNPDIEGGDDDTGFFLQDDADFLQNEGTVDVKGM

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=65850 AMJAP_RS01300 WP_019620984.1 276089..277255(+) (pilU) [Amphritea japonica ATCC BAA-1530]
GTGGATATTACTCAACTGCTCAAGGTTATGACTGACCGTGACGCTTCCGATCTATTTATTACCGCCGGTGCCAGGCCCAG
TATTAAGATTGATGGCACGATTAAACCGTTGACCAAAGAGTCGCTGAAACCCGCACAGGCCCGATCGCTGACTTACAGCA
CGATGAATGATAAGCAGTTGTCGGAATTTGAGGGAACCCGGGAGTGTAACTTTGCCATCAGTGCCCAGGGCATTGGTCGA
TTCCGGGTGAGTGCTTTTTTCCAGCGCAACTCCCCTGGTATGGTACTACGCCGAATCAATACCTATATCCCTAGTCTGGA
AGAGCTAAACCTGCCGCCGGTGCTGAAAGATCTGGCGATGACTAAACGGGGCTTGATTCTGTTTGTGGGTGGTACCAGTA
CGGGTAAATCGACCTCTTTGGCATCGATGATCGATTATCGGAATACGAATTCAGCCGGGCACATCATCACCATTGAGGAT
CCGATTGAGTATATCCACGAGCATAAACAGAGTGTCATAACTCAGCGGGAAGTAGGTCTGGATACCGAATCCTTTGAGAT
TGCACTTAAAAATACCCTGCGTCAGGCACCTGATGTGATTCTGATGGGTGAGATTCGTACCGCGGACACGATGGGATACG
GTCTGACTTTCGCTGAAACGGGGCATCTGTGTATGGCGACCCTGCACGCGAATAACGCTAACCAGGCGCTTGATCGAATT
ATCAGTTTCTTTCCGCCTGAGCATCATGCGCAGATCTGGATGGATCTGTCGCTTAACCTGAAGGCGATTGTCGCACAGCA
ATTGCTGCCAACCAAAGACGGTAAAGGGCGCCGGGCAGTGGTGGAAGTGCTGATCAATACGCCATTGATGCAGGACCTGA
TTCGTAAGGGTGAGGTGCACGAGCTGAAAGAGGTGATCAAGAAATCCACTAACCTGGGAATGCAGACCTTTGATCAGGCC
CTTTACAACGAATATAAGGAAGGCAACATCAATTACGATGTGGCGATCGCGCACGCGGATTCACCTAATGATCTGCGTCT
GATGATAAAACTGAATGCTGATACTAACCCTGATATTGAAGGGGGTGATGATGATACCGGGTTCTTCCTCCAGGATGACG
CGGACTTCCTGCAAAATGAAGGCACAGTGGATGTAAAAGGGATGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.782

97.165

0.639
  pilU Acinetobacter baylyi ADP1

62.745

92.01

0.577
  pilU Vibrio cholerae strain A1552

57.429

90.206

0.518
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.058

88.918

0.392
  pilT Acinetobacter baylyi ADP1

41.813

88.144

0.369
  pilT Pseudomonas aeruginosa PAK

41.159

88.918

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.58

88.918

0.361

Multiple sequence alignment