Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AMJAP_RS01295 Genome accession   NZ_AP014545
Coordinates   275039..276073 (+) Length   344 a.a.
NCBI ID   WP_019620985.1    Uniprot ID   A0A7R6P0F4
Organism   Amphritea japonica ATCC BAA-1530     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 270039..281073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMJAP_RS01265 (AMJAP_0238) metW 270262..270852 (-) 591 WP_019620991.1 methionine biosynthesis protein MetW -
  AMJAP_RS01270 (AMJAP_0239) metX 270852..272018 (-) 1167 WP_019620990.1 homoserine O-succinyltransferase MetX -
  AMJAP_RS01275 (AMJAP_0240) - 272159..272452 (-) 294 WP_019620989.1 DUF167 domain-containing protein -
  AMJAP_RS01280 (AMJAP_0241) - 272459..273016 (-) 558 WP_019620988.1 YggT family protein -
  AMJAP_RS01285 (AMJAP_0242) proC 273132..273956 (-) 825 WP_019620987.1 pyrroline-5-carboxylate reductase -
  AMJAP_RS01290 (AMJAP_0243) - 274014..274712 (-) 699 WP_019620986.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMJAP_RS01295 (AMJAP_0244) pilT 275039..276073 (+) 1035 WP_019620985.1 type IV pilus twitching motility protein PilT Machinery gene
  AMJAP_RS01300 (AMJAP_0245) pilU 276089..277255 (+) 1167 WP_019620984.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMJAP_RS01305 (AMJAP_0246) - 277326..278609 (-) 1284 WP_019620983.1 dihydroorotase -
  AMJAP_RS01310 (AMJAP_0247) - 278614..279615 (-) 1002 WP_019620982.1 aspartate carbamoyltransferase catalytic subunit -
  AMJAP_RS01315 (AMJAP_0248) pyrR 279636..280151 (-) 516 WP_019620981.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AMJAP_RS01320 (AMJAP_0249) ruvX 280132..280608 (-) 477 WP_019620980.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38200.86 Da        Isoelectric Point: 6.7785

>NTDB_id=65849 AMJAP_RS01295 WP_019620985.1 275039..276073(+) (pilT) [Amphritea japonica ATCC BAA-1530]
MDITELLSFTVQQGASDLHITAGMPPVIRVDGEVKRIKLPSLEHKQVHTLIYDIMNDRQRKDYEERFETDFSFEVPGLAR
FRVNAFNQNRGAAAVFRTIPSKVLTMDDLGMGQVFRNLSDNPRGLVLVTGPTGSGKSTTLAAMVDYVNETRNDHILTIED
PIEFVHESKKCLINQREVHRDTLGFEEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKSMVRSMLSESLQGVISQTLLKKPKGGRIAAHEIMVGTPAIRNLIREDKVAQMYSAIQTGAGFGMITLDQSL
TDLMQKGLITRDTARGKALNPKNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=65849 AMJAP_RS01295 WP_019620985.1 275039..276073(+) (pilT) [Amphritea japonica ATCC BAA-1530]
ATGGATATTACAGAACTCTTATCGTTTACCGTTCAGCAGGGAGCATCTGACCTTCATATTACCGCCGGTATGCCACCGGT
CATACGTGTGGATGGTGAAGTGAAGCGTATTAAGCTGCCCTCTCTGGAGCATAAGCAGGTACATACGCTGATCTACGATA
TTATGAATGACCGGCAGCGTAAGGATTATGAGGAACGGTTTGAAACCGATTTCTCTTTTGAAGTACCCGGTTTAGCACGA
TTTCGTGTTAACGCATTCAACCAGAACCGTGGTGCAGCGGCGGTATTCCGTACTATCCCAAGTAAAGTGCTGACTATGGA
TGATCTGGGTATGGGGCAGGTGTTCCGTAACCTGTCTGATAACCCACGGGGGCTGGTGTTGGTAACCGGACCAACCGGTT
CAGGTAAGAGTACCACCCTGGCCGCGATGGTCGATTATGTGAATGAAACCCGCAACGATCATATACTGACTATCGAAGAC
CCGATTGAATTTGTTCACGAAAGTAAGAAATGTCTGATTAACCAGCGAGAGGTTCACCGGGACACTCTGGGCTTTGAAGA
AGCCCTGCGCTCCGCCCTGCGTGAGGATCCCGATGTTATTCTGGTGGGTGAGATGCGAGATCTGGAAACTATCCGCTTAG
CGCTGACCGCCGCAGAAACCGGGCACCTGGTTTTTGGCACACTGCATACGACCTCGGCCGCTAAAACGATTGACCGTATC
ATTGATGTGTTTCCCGCCGCGGAAAAGTCGATGGTTCGTTCGATGCTGTCAGAGTCTTTACAAGGTGTTATTTCTCAGAC
GTTGTTGAAAAAGCCTAAAGGCGGTCGAATTGCAGCGCATGAGATTATGGTTGGTACTCCGGCTATCCGAAACCTGATTC
GTGAGGATAAGGTTGCGCAGATGTATTCAGCGATTCAGACCGGTGCGGGCTTTGGTATGATTACTCTGGATCAATCGCTG
ACGGATCTGATGCAGAAAGGCCTGATTACCCGTGATACTGCGCGTGGTAAAGCGTTGAATCCAAAGAATTTCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.977

100

0.82
  pilT Pseudomonas stutzeri DSM 10701

81.686

100

0.817
  pilT Acinetobacter baumannii D1279779

80.523

100

0.805
  pilT Acinetobacter baumannii strain A118

80.523

100

0.805
  pilT Acinetobacter nosocomialis M2

80.523

100

0.805
  pilT Acinetobacter baylyi ADP1

79.36

100

0.794
  pilT Legionella pneumophila strain Lp02

73.256

100

0.733
  pilT Legionella pneumophila strain ERS1305867

73.256

100

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.766

97.965

0.654
  pilT Vibrio cholerae strain A1552

66.766

97.965

0.654
  pilT Neisseria meningitidis 8013

63.768

100

0.64
  pilT Neisseria gonorrhoeae MS11

63.478

100

0.637
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Vibrio cholerae strain A1552

41.846

94.477

0.395

Multiple sequence alignment