Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MJX77_RS03685 Genome accession   NZ_CP092454
Coordinates   779175..780887 (+) Length   570 a.a.
NCBI ID   WP_005301829.1    Uniprot ID   -
Organism   Acinetobacter sp. AHP123     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 774175..785887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJX77_RS03650 nusA 775062..776546 (-) 1485 WP_003654584.1 transcription termination factor NusA -
  MJX77_RS03655 rimP 776583..777110 (-) 528 WP_004795010.1 ribosome maturation factor RimP -
  MJX77_RS03675 secG 777747..778076 (-) 330 WP_004795012.1 preprotein translocase subunit SecG -
  MJX77_RS03680 tpiA 778089..778883 (-) 795 WP_005301831.1 triose-phosphate isomerase -
  MJX77_RS03685 pilB 779175..780887 (+) 1713 WP_005301829.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MJX77_RS03690 pilC 780915..782141 (+) 1227 WP_252060577.1 type II secretion system F family protein Machinery gene
  MJX77_RS19320 pilD 782141..783001 (+) 861 WP_286089233.1 A24 family peptidase Machinery gene
  MJX77_RS03705 coaE 783003..783599 (+) 597 WP_151821432.1 dephospho-CoA kinase -
  MJX77_RS03710 - 783596..784510 (-) 915 WP_252060578.1 DMT family transporter -
  MJX77_RS03715 rlmB 784546..785295 (-) 750 WP_151826698.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MJX77_RS03720 - 785398..785724 (-) 327 WP_004795031.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63507.87 Da        Isoelectric Point: 4.9650

>NTDB_id=657709 MJX77_RS03685 WP_005301829.1 779175..780887(+) (pilB) [Acinetobacter sp. AHP123]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQAALDAAKRAKQDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLVQRGQILYVATSNPSNIDAIDAIRFNSKLMVEPVIVEHHKLEKILVQQFTEESNFEFSDEEF
DLDVDLDTSTAPEEDEDTPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKVPTDIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=657709 MJX77_RS03685 WP_005301829.1 779175..780887(+) (pilB) [Acinetobacter sp. AHP123]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTTGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAGCAGCACTAGATGCAGCAAAAAGAGCTAAACAGGATACTGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGACGTTTACGATACTAGCCAGATT
CCTAAAGATTTGGTCGATCAGAAACTGATTTTAAAACATCGGATCTTGCCTCTCGTACAAAGAGGACAGATTTTATATGT
TGCAACCAGTAATCCCAGCAATATTGATGCTATTGATGCTATTCGCTTCAACAGCAAGCTTATGGTTGAGCCGGTTATTG
TCGAGCACCATAAACTCGAGAAAATTCTGGTACAGCAATTTACTGAAGAAAGTAATTTCGAGTTTAGTGATGAAGAGTTT
GATCTAGATGTAGATCTTGATACCTCAACTGCTCCAGAAGAGGATGAGGATACTCCACAAGGCGATGAAGCACCTATTGT
TAAATATATTAACAAGTTATTGATTGATGCGATTCGTATGGGGGCTTCTGATTTACATTTCGAACCTTATGAAAAATCGT
ATCGGGTCCGTTATCGGGTCGATGGCGTTTTGCGTCAAATTGCAAATCCACCTCTGCAACTGGCTAATCGTTTGGCTTCA
CGCTTAAAAGTGATGTCTCAAATGGACATTTCTGAAAAGCGTGTCCCTCAAGATGGTCGTATCAAGCTCAAGCTATCAAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACCTTATTTGGTGAAAAGCTAGTTCTACGTATTCTCGATCCAT
CGAGTGCTATGCTGGGAATTGATGCTCTAGGATATGAAGAAGAGCAAAAAGCGCTCTTTATGGAAGCATTAGATAAGCCA
CAAGGTATGCTTTTAATCACAGGTCCGACAGGTTCTGGTAAAACAGTATCTTTATATACAGGCCTCAATATCTTAAATAC
TGAAAGTTCTAATATTTCTACTGCCGAAGATCCAGTCGAAATTAACCTTGAAGGCATTAATCAGGTTAACGTGAACCCTA
AGGTAGGTCTGACTTTTTCAGCTGCACTTAAGTCATTTTTACGTCAGGACCCTGACATTATTATGGTCGGCGAGATTCGT
GATTTAGAAACAGCTGAAATCGCGATTAAAGCAGCTCAGACAGGTCATATGGTGATGTCCACGCTACATACCAACAGTGC
TCCTGAAACATTGACTCGTCTACGCAATATGGGGGTTCCGTCTTTCAATATTGCGACATCGGTCAATCTGGTCATTGCTC
AGCGTTTGGCACGTCGACTATGTTCTCAATGTAAAGTACCTACTGACATCCCTAAACAAAGCCTATTGGAAATGGGTTTT
ACTGAACAAGATCTGACACATCCCGATTTTCAAGTTTTTCAACCAGTCGGTTGTCCAGAATGTCGTGAGGGTTACAAAGG
TCGAGTTGGTATTTATGAAGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTACGTCGCTCAGGTTTAAAGAAAGTTATGCAAGGTGTTACTTCT
TTACAAGAAGTCAATCGCGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.561

100

0.946
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

51.937

99.649

0.518
  pilF Neisseria gonorrhoeae MS11

49.11

98.596

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

51.901

92.281

0.479
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.289

91.053

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.342

100

0.381
  pilF Thermus thermophilus HB27

36.964

98.246

0.363