Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   MIS33_RS09245 Genome accession   NZ_CP092359
Coordinates   1855799..1857085 (-) Length   428 a.a.
NCBI ID   WP_249446593.1    Uniprot ID   -
Organism   Wielerella bovis strain 08LB461     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1850799..1862085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS33_RS09215 (MIS33_09215) murB 1851239..1852270 (-) 1032 WP_249446591.1 UDP-N-acetylmuramate dehydrogenase -
  MIS33_RS09220 (MIS33_09220) - 1852286..1852906 (-) 621 WP_249442246.1 L-threonylcarbamoyladenylate synthase -
  MIS33_RS09225 (MIS33_09225) pncC 1852981..1853460 (-) 480 WP_249442247.1 nicotinamide-nucleotide amidase -
  MIS33_RS09230 (MIS33_09230) zapD 1853513..1854259 (-) 747 WP_249442248.1 cell division protein ZapD -
  MIS33_RS09235 (MIS33_09235) coaE 1854272..1854886 (-) 615 WP_249442249.1 dephospho-CoA kinase -
  MIS33_RS09240 (MIS33_09240) pilD 1854890..1855789 (-) 900 WP_249442250.1 A24 family peptidase Machinery gene
  MIS33_RS09245 (MIS33_09245) pilG 1855799..1857085 (-) 1287 WP_249446593.1 type II secretion system F family protein Machinery gene
  MIS33_RS09250 (MIS33_09250) pilF 1857110..1858789 (-) 1680 WP_249442252.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MIS33_RS09255 (MIS33_09255) - 1858969..1859112 (-) 144 WP_249446594.1 hypothetical protein -
  MIS33_RS09260 (MIS33_09260) - 1859373..1859753 (-) 381 WP_249446595.1 CrcB family protein -
  MIS33_RS09265 (MIS33_09265) - 1859750..1860571 (-) 822 WP_249446596.1 ferritin-like domain-containing protein -
  MIS33_RS09270 (MIS33_09270) lolA 1860735..1861349 (+) 615 WP_283397657.1 outer membrane lipoprotein chaperone LolA -

Sequence

Protein


Download         Length: 428 a.a.        Molecular weight: 46912.23 Da        Isoelectric Point: 9.9117

>NTDB_id=656731 MIS33_RS09245 WP_249446593.1 1855799..1857085(-) (pilG) [Wielerella bovis strain 08LB461]
MAEARKSAKSAAKKPVKKTAAKSAKPEKKEKGNRYQFEGKNLQTENIVRGEVVAKNEDEARQKLARRSIKVMQLTKMKKA
RAKKITQADITIFTRQLSTMMKAGLPMMQAFDIVAKGNPNANMTNLLLDIRNRVEQGESLGDSFAAHPAYFDKFYCNLVA
AGEAGGVLEGLLDKLATYKEKTEAIKKKVKGALTYPIAVMIVAIALILVMMMFVLPSFKKIYEGLGAELPGLTQWMMNAS
DFFVEKGWIIIPGLILGTWGLIQFHKHSFAFQKRVDAWLLKMPIFGEIVMKATIARWSRTTATLFTAGVPLVEVLDSVAG
AAGNIIYEEATHEIRSKVNQGISLTSGMQGANVFPNMVVQMASIGEESGALDDMLNKVAEFYEEEVDNAVATLSSLMEPI
IMVVLGSIVGVILVAMYLPLFSMGDAIG

Nucleotide


Download         Length: 1287 bp        

>NTDB_id=656731 MIS33_RS09245 WP_249446593.1 1855799..1857085(-) (pilG) [Wielerella bovis strain 08LB461]
ATGGCAGAAGCAAGAAAATCGGCAAAATCCGCCGCGAAAAAGCCAGTGAAAAAAACTGCAGCAAAATCAGCAAAACCTGA
AAAGAAAGAAAAAGGTAACCGCTATCAATTTGAAGGTAAGAACTTACAGACTGAAAATATTGTTCGCGGTGAGGTGGTTG
CAAAAAACGAAGATGAGGCACGTCAGAAGTTAGCGCGTCGCTCCATTAAAGTGATGCAATTAACCAAAATGAAAAAGGCG
CGTGCGAAGAAAATCACACAAGCTGATATTACCATCTTTACTCGCCAACTTTCTACTATGATGAAAGCGGGTTTGCCTAT
GATGCAAGCATTTGATATTGTGGCAAAAGGTAATCCAAATGCGAATATGACTAATTTATTGTTGGATATTCGTAATCGAG
TGGAGCAAGGTGAATCTTTGGGTGATTCTTTTGCTGCACATCCTGCTTATTTTGACAAATTCTATTGCAATTTGGTTGCA
GCAGGTGAAGCAGGTGGTGTGTTGGAGGGTTTGCTGGATAAATTGGCAACCTATAAAGAAAAAACGGAAGCTATTAAAAA
GAAAGTAAAAGGTGCTTTAACGTATCCGATTGCGGTGATGATAGTCGCAATTGCTTTGATTTTGGTTATGATGATGTTCG
TGTTGCCATCATTTAAGAAAATTTATGAAGGTTTGGGAGCAGAGCTGCCAGGTTTAACACAATGGATGATGAATGCGTCT
GATTTCTTTGTAGAAAAAGGTTGGATTATTATTCCTGGATTAATTTTGGGTACATGGGGCTTAATTCAATTCCATAAACA
TTCTTTTGCTTTCCAAAAACGGGTAGATGCTTGGTTATTAAAAATGCCTATTTTTGGCGAGATTGTGATGAAAGCAACCA
TTGCACGTTGGTCGCGTACAACTGCAACTTTGTTTACTGCTGGTGTGCCTTTAGTAGAGGTATTGGATTCGGTAGCAGGT
GCTGCTGGTAATATTATTTATGAGGAAGCTACTCATGAAATTCGCAGTAAAGTAAACCAAGGCATTTCTTTGACCTCAGG
TATGCAAGGTGCGAATGTCTTCCCTAATATGGTGGTACAAATGGCATCTATTGGTGAAGAATCAGGTGCTCTGGACGATA
TGTTGAACAAAGTAGCAGAATTCTATGAGGAAGAAGTAGATAATGCTGTTGCCACTTTATCTTCGTTGATGGAACCAATT
ATTATGGTGGTATTAGGTAGTATCGTTGGTGTGATTCTGGTTGCGATGTACTTGCCACTGTTCAGCATGGGCGATGCCAT
CGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(93-216)

Type II secretion system (T2SS), protein F (T2SSF)

(297-419)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

63.25

93.458

0.591
  pilG Neisseria meningitidis 44/76-A

62.5

93.458

0.584
  pilC Legionella pneumophila strain ERS1305867

47.487

92.991

0.442
  pilC Pseudomonas stutzeri DSM 10701

43.812

94.393

0.414
  pilC Acinetobacter baumannii D1279779

45.455

89.953

0.409
  pilC Acinetobacter baylyi ADP1

44.444

90.421

0.402