Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MIS33_RS09250 Genome accession   NZ_CP092359
Coordinates   1857110..1858789 (-) Length   559 a.a.
NCBI ID   WP_249442252.1    Uniprot ID   -
Organism   Wielerella bovis strain 08LB461     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1852110..1863789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS33_RS09220 (MIS33_09220) - 1852286..1852906 (-) 621 WP_249442246.1 L-threonylcarbamoyladenylate synthase -
  MIS33_RS09225 (MIS33_09225) pncC 1852981..1853460 (-) 480 WP_249442247.1 nicotinamide-nucleotide amidase -
  MIS33_RS09230 (MIS33_09230) zapD 1853513..1854259 (-) 747 WP_249442248.1 cell division protein ZapD -
  MIS33_RS09235 (MIS33_09235) coaE 1854272..1854886 (-) 615 WP_249442249.1 dephospho-CoA kinase -
  MIS33_RS09240 (MIS33_09240) pilD 1854890..1855789 (-) 900 WP_249442250.1 A24 family peptidase Machinery gene
  MIS33_RS09245 (MIS33_09245) pilG 1855799..1857085 (-) 1287 WP_249446593.1 type II secretion system F family protein Machinery gene
  MIS33_RS09250 (MIS33_09250) pilF 1857110..1858789 (-) 1680 WP_249442252.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MIS33_RS09255 (MIS33_09255) - 1858969..1859112 (-) 144 WP_249446594.1 hypothetical protein -
  MIS33_RS09260 (MIS33_09260) - 1859373..1859753 (-) 381 WP_249446595.1 CrcB family protein -
  MIS33_RS09265 (MIS33_09265) - 1859750..1860571 (-) 822 WP_249446596.1 ferritin-like domain-containing protein -
  MIS33_RS09270 (MIS33_09270) lolA 1860735..1861349 (+) 615 WP_283397657.1 outer membrane lipoprotein chaperone LolA -
  MIS33_RS09275 (MIS33_09275) - 1861414..1862517 (+) 1104 WP_249443836.1 anhydro-N-acetylmuramic acid kinase -
  MIS33_RS09280 (MIS33_09280) rbfA 1862760..1863131 (+) 372 WP_249442255.1 30S ribosome-binding factor RbfA -
  MIS33_RS09285 (MIS33_09285) - 1863186..1863590 (-) 405 WP_249446597.1 thioredoxin domain-containing protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62035.87 Da        Isoelectric Point: 5.2149

>NTDB_id=656732 MIS33_RS09250 WP_249442252.1 1857110..1858789(-) (pilF) [Wielerella bovis strain 08LB461]
MSVGLLRVLVQQKLVPQDKIEKYQTAIKENKPVLPLVFGDKIISPEALAELCSSLFNYPMLDLNYFPRSAIVQDLIDEAQ
MQELRAIPVLKRGRKVFFAVSDPTQIQNVQKAVFASGLQVDLVIARDDQLTTVLEWFGQSNSALLKEMGMEVNKEEAKQT
MIVDGQEEEDGPVARFIQKILHDAVTSGASDIHFEFYEFMARVRFRTDGVLREVVQPPLQLRSQLASRIKVMAKLDISEK
RVPQDGRIQLQFSKNTKAIDFRVNTMPCLFGEKVVMRILNSDAASLNIDQLGFEPFQKELIMEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTDDINISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDVIMIGEIRDLGTADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCSACKAPMERPPEKALLEVGFTEEDLAQDWTM
YRPVGCDACKGKGYKGRAGLYEVMKMSEKMKAVIMSGGTEVDIANIAYEDGLVDLRRSGLLKVMQGITSLEEVIATTNE

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=656732 MIS33_RS09250 WP_249442252.1 1857110..1858789(-) (pilF) [Wielerella bovis strain 08LB461]
ATGAGCGTAGGTTTATTGCGGGTCTTGGTGCAACAAAAATTAGTCCCACAAGATAAAATTGAAAAATATCAAACAGCTAT
CAAAGAAAACAAACCTGTTTTGCCTTTGGTGTTTGGCGATAAAATTATTTCGCCTGAAGCATTGGCAGAATTGTGTTCGT
CTTTATTCAACTATCCGATGTTGGATTTAAACTATTTTCCACGCAGTGCGATTGTGCAAGATTTGATTGATGAAGCACAA
ATGCAAGAATTGCGTGCGATTCCTGTGTTAAAACGTGGTCGAAAAGTGTTCTTCGCTGTTTCAGACCCTACACAAATTCA
AAATGTACAAAAAGCGGTATTTGCCAGCGGTTTGCAAGTGGATTTGGTCATCGCGCGTGATGACCAATTAACTACTGTGT
TGGAATGGTTTGGTCAAAGTAACTCTGCTTTGCTCAAAGAAATGGGCATGGAGGTAAATAAAGAAGAAGCCAAACAGACC
ATGATTGTGGATGGACAGGAAGAAGAGGATGGTCCTGTTGCACGGTTTATTCAAAAAATCTTGCATGATGCGGTTACTTC
TGGCGCATCTGATATTCACTTTGAGTTTTACGAATTTATGGCGCGTGTGCGTTTTCGTACCGATGGTGTGTTGCGCGAAG
TGGTGCAACCGCCCTTGCAACTGCGTAGCCAGTTGGCATCGCGTATTAAAGTGATGGCGAAATTGGATATTTCTGAAAAA
CGCGTACCGCAGGACGGACGTATTCAGTTGCAATTTTCTAAAAATACCAAAGCGATTGACTTTCGTGTGAATACTATGCC
GTGTTTGTTTGGTGAAAAAGTCGTGATGCGTATTTTGAACTCGGATGCAGCCAGCCTGAATATTGACCAGTTGGGTTTTG
AACCTTTCCAAAAAGAATTAATTATGGAGGCGATTCATCGCCCATACGGTATGGTGTTGGTAACAGGCCCAACGGGTTCG
GGTAAAACGGTTTCGCTCTATACTTGTTTGAACATTCTGAATACTGATGACATCAATATTTCTACTGCCGAAGACCCTGC
GGAGATTAACTTGCCGGGGATTAACCAAGTAAACGTAAATGATAAACAAGGTTTAACTTTTGAGGCAGCCTTAAAAGCAT
TCCTGCGTCAAGACCCTGATGTAATTATGATTGGTGAGATTCGTGACTTGGGTACGGCGGATATTGCAATTAAAGCAGCG
CAAACAGGTCACATGGTATTCTCAACCTTGCACACCAATAATGCACCAGCTACTTTGTCGCGTATGTTAAATATGGGTGT
AGCACCATTTAATATTGCTTCGGCGGTAAACTTGATTATGGCACAACGTTTGGCGCGTCGTTTATGTTCTGCTTGCAAAG
CACCGATGGAGCGTCCACCTGAAAAAGCTTTATTAGAAGTAGGCTTTACTGAGGAGGATTTAGCGCAGGATTGGACAATG
TATCGCCCTGTGGGTTGTGATGCTTGTAAAGGTAAAGGCTATAAAGGTCGTGCAGGTTTGTATGAAGTAATGAAAATGTC
TGAAAAAATGAAAGCTGTCATCATGAGTGGCGGTACGGAGGTGGATATTGCTAATATCGCATATGAAGATGGTTTGGTAG
ATTTGCGTCGTTCAGGTTTACTGAAAGTTATGCAAGGTATTACTTCTTTGGAAGAAGTTATTGCAACCACAAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-140)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.231

100

0.692
  pilB Acinetobacter baumannii D1279779

53.427

100

0.544
  pilB Acinetobacter baylyi ADP1

52.465

100

0.533
  pilB Legionella pneumophila strain ERS1305867

47.679

100

0.478
  pilB Vibrio cholerae strain A1552

46.372

100

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

46.113

100

0.467
  pilB Vibrio campbellii strain DS40M4

47.793

93.202

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.652

92.487

0.367