Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   MIS46_RS08925 Genome accession   NZ_CP092358
Coordinates   1803823..1805109 (-) Length   428 a.a.
NCBI ID   WP_249442251.1    Uniprot ID   -
Organism   Wielerella bovis strain 09LB414     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1798823..1810109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS46_RS08895 (MIS46_08895) murB 1799263..1800294 (-) 1032 WP_249442245.1 UDP-N-acetylmuramate dehydrogenase -
  MIS46_RS08900 (MIS46_08900) - 1800310..1800930 (-) 621 WP_249442246.1 L-threonylcarbamoyladenylate synthase -
  MIS46_RS08905 (MIS46_08905) pncC 1801005..1801484 (-) 480 WP_249442247.1 nicotinamide-nucleotide amidase -
  MIS46_RS08910 (MIS46_08910) zapD 1801537..1802283 (-) 747 WP_249442248.1 cell division protein ZapD -
  MIS46_RS08915 (MIS46_08915) coaE 1802296..1802910 (-) 615 WP_249442249.1 dephospho-CoA kinase -
  MIS46_RS08920 (MIS46_08920) pilD 1802914..1803813 (-) 900 WP_249442250.1 A24 family peptidase Machinery gene
  MIS46_RS08925 (MIS46_08925) pilG 1803823..1805109 (-) 1287 WP_249442251.1 type II secretion system F family protein Machinery gene
  MIS46_RS08930 (MIS46_08930) pilF 1805134..1806813 (-) 1680 WP_249442252.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MIS46_RS08935 (MIS46_08935) - 1806993..1807229 (-) 237 WP_249442253.1 hypothetical protein -
  MIS46_RS11475 - 1807284..1807412 (-) 129 WP_283397261.1 hypothetical protein -
  MIS46_RS08940 (MIS46_08940) - 1807409..1807789 (-) 381 WP_249442254.1 CrcB family protein -
  MIS46_RS08945 (MIS46_08945) - 1807786..1808607 (-) 822 WP_249443834.1 ferritin-like domain-containing protein -
  MIS46_RS08950 (MIS46_08950) lolA 1808771..1809385 (+) 615 WP_283397262.1 outer membrane lipoprotein chaperone LolA -

Sequence

Protein


Download         Length: 428 a.a.        Molecular weight: 46988.30 Da        Isoelectric Point: 9.8379

>NTDB_id=656704 MIS46_RS08925 WP_249442251.1 1803823..1805109(-) (pilG) [Wielerella bovis strain 09LB414]
MAEARKSAKSAAKKPVKKTAAKSAKPEKKEKGNRYQFEGKNLQTENIVRGEVVAKNEDEARQKLARRSIKVMQLTKMKKA
RAKKITQADITIFTRQLSTMMKAGLPMMQAFDIVAKGNPNANMTNLLMDIRNRVEQGESLGDSFAEHPAYFDKFYCNLVA
AGEAGGVLEGLLDKLATYKEKTEAIKKKVKGALTYPIAVMIVAIALILVMMMFVLPSFKKIYEGLGAELPGLTQWMMNAS
DFFVEKGWIIIPGLILGTWGLIQFHKHSFAFQKRVDAWLLKMPIFGEIVMKATIARWSRTTATLFTAGVPLVEVLDSVAG
AAGNIIYEEATHEIRSKVNQGISLTSGMQGANVFPNMVVQMASIGEESGALDDMLNKVAEFYEEEVDNAVATLSSLMEPI
IMVVLGSIVGVILVAMYLPLFSMGDAIG

Nucleotide


Download         Length: 1287 bp        

>NTDB_id=656704 MIS46_RS08925 WP_249442251.1 1803823..1805109(-) (pilG) [Wielerella bovis strain 09LB414]
ATGGCAGAAGCAAGAAAATCGGCAAAATCCGCCGCTAAAAAGCCAGTGAAAAAAACTGCAGCAAAATCAGCAAAACCTGA
AAAGAAAGAAAAAGGTAACCGCTATCAATTTGAAGGTAAGAACTTACAGACTGAAAATATTGTTCGCGGTGAGGTGGTTG
CAAAAAACGAAGATGAGGCACGTCAGAAGTTAGCGCGTCGCTCCATTAAAGTGATGCAATTAACCAAAATGAAAAAGGCG
CGTGCGAAGAAAATCACACAAGCTGATATTACTATCTTTACTCGCCAACTTTCTACTATGATGAAAGCGGGTTTGCCCAT
GATGCAAGCATTTGATATTGTGGCAAAAGGTAATCCAAATGCGAATATGACAAATTTGTTGATGGATATTCGTAACCGAG
TTGAGCAAGGTGAATCTTTGGGTGATTCCTTTGCTGAACATCCTGCGTATTTTGATAAATTTTATTGCAATTTGGTTGCA
GCAGGTGAAGCAGGTGGTGTGTTGGAGGGTTTGCTGGATAAATTGGCAACCTATAAAGAAAAAACGGAAGCTATTAAAAA
GAAAGTAAAAGGTGCTTTAACGTATCCGATTGCGGTGATGATAGTCGCAATTGCTTTGATTTTGGTTATGATGATGTTCG
TGTTGCCATCATTTAAGAAAATTTATGAAGGTTTGGGAGCAGAGCTGCCAGGTTTAACACAATGGATGATGAATGCGTCT
GATTTCTTTGTAGAAAAAGGTTGGATTATTATTCCTGGATTAATTTTGGGTACATGGGGCTTAATTCAATTCCATAAACA
TTCTTTTGCTTTCCAAAAACGGGTAGATGCTTGGTTATTAAAAATGCCTATTTTTGGCGAGATTGTGATGAAAGCAACCA
TTGCACGTTGGTCGCGTACAACTGCAACTTTGTTTACTGCTGGTGTGCCTTTAGTAGAGGTATTGGATTCGGTAGCAGGT
GCTGCTGGTAATATTATTTATGAGGAAGCTACTCATGAAATTCGCAGTAAAGTAAACCAAGGCATTTCTTTGACCTCAGG
TATGCAAGGTGCGAATGTCTTCCCTAATATGGTGGTACAAATGGCATCTATTGGTGAAGAATCAGGTGCTCTGGACGATA
TGTTGAACAAAGTAGCAGAATTCTATGAGGAAGAAGTAGATAATGCTGTTGCCACTTTATCTTCGTTGATGGAACCAATT
ATTATGGTGGTATTAGGTAGTATCGTTGGTGTGATTCTGGTTGCGATGTACTTGCCACTGTTCAGCATGGGCGATGCCAT
CGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(93-216)

Type II secretion system (T2SS), protein F (T2SSF)

(297-419)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

63.5

93.458

0.593
  pilG Neisseria meningitidis 44/76-A

62.75

93.458

0.586
  pilC Legionella pneumophila strain ERS1305867

47.487

92.991

0.442
  pilC Pseudomonas stutzeri DSM 10701

43.812

94.393

0.414
  pilC Acinetobacter baumannii D1279779

45.195

89.953

0.407
  pilC Acinetobacter baylyi ADP1

44.186

90.421

0.4
  pilC Vibrio campbellii strain DS40M4

36.817

98.364

0.362