Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MIS46_RS08930 Genome accession   NZ_CP092358
Coordinates   1805134..1806813 (-) Length   559 a.a.
NCBI ID   WP_249442252.1    Uniprot ID   -
Organism   Wielerella bovis strain 09LB414     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1800134..1811813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS46_RS08900 (MIS46_08900) - 1800310..1800930 (-) 621 WP_249442246.1 L-threonylcarbamoyladenylate synthase -
  MIS46_RS08905 (MIS46_08905) pncC 1801005..1801484 (-) 480 WP_249442247.1 nicotinamide-nucleotide amidase -
  MIS46_RS08910 (MIS46_08910) zapD 1801537..1802283 (-) 747 WP_249442248.1 cell division protein ZapD -
  MIS46_RS08915 (MIS46_08915) coaE 1802296..1802910 (-) 615 WP_249442249.1 dephospho-CoA kinase -
  MIS46_RS08920 (MIS46_08920) pilD 1802914..1803813 (-) 900 WP_249442250.1 A24 family peptidase Machinery gene
  MIS46_RS08925 (MIS46_08925) pilG 1803823..1805109 (-) 1287 WP_249442251.1 type II secretion system F family protein Machinery gene
  MIS46_RS08930 (MIS46_08930) pilF 1805134..1806813 (-) 1680 WP_249442252.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MIS46_RS08935 (MIS46_08935) - 1806993..1807229 (-) 237 WP_249442253.1 hypothetical protein -
  MIS46_RS11475 - 1807284..1807412 (-) 129 WP_283397261.1 hypothetical protein -
  MIS46_RS08940 (MIS46_08940) - 1807409..1807789 (-) 381 WP_249442254.1 CrcB family protein -
  MIS46_RS08945 (MIS46_08945) - 1807786..1808607 (-) 822 WP_249443834.1 ferritin-like domain-containing protein -
  MIS46_RS08950 (MIS46_08950) lolA 1808771..1809385 (+) 615 WP_283397262.1 outer membrane lipoprotein chaperone LolA -
  MIS46_RS08955 (MIS46_08955) - 1809450..1810553 (+) 1104 WP_249443836.1 anhydro-N-acetylmuramic acid kinase -
  MIS46_RS08960 (MIS46_08960) rbfA 1810796..1811167 (+) 372 WP_249442255.1 30S ribosome-binding factor RbfA -
  MIS46_RS08965 (MIS46_08965) - 1811222..1811626 (-) 405 WP_249442256.1 thioredoxin domain-containing protein -
  MIS46_RS11480 - 1811587..1811715 (-) 129 WP_283397263.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62035.87 Da        Isoelectric Point: 5.2149

>NTDB_id=656705 MIS46_RS08930 WP_249442252.1 1805134..1806813(-) (pilF) [Wielerella bovis strain 09LB414]
MSVGLLRVLVQQKLVPQDKIEKYQTAIKENKPVLPLVFGDKIISPEALAELCSSLFNYPMLDLNYFPRSAIVQDLIDEAQ
MQELRAIPVLKRGRKVFFAVSDPTQIQNVQKAVFASGLQVDLVIARDDQLTTVLEWFGQSNSALLKEMGMEVNKEEAKQT
MIVDGQEEEDGPVARFIQKILHDAVTSGASDIHFEFYEFMARVRFRTDGVLREVVQPPLQLRSQLASRIKVMAKLDISEK
RVPQDGRIQLQFSKNTKAIDFRVNTMPCLFGEKVVMRILNSDAASLNIDQLGFEPFQKELIMEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTDDINISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDVIMIGEIRDLGTADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCSACKAPMERPPEKALLEVGFTEEDLAQDWTM
YRPVGCDACKGKGYKGRAGLYEVMKMSEKMKAVIMSGGTEVDIANIAYEDGLVDLRRSGLLKVMQGITSLEEVIATTNE

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=656705 MIS46_RS08930 WP_249442252.1 1805134..1806813(-) (pilF) [Wielerella bovis strain 09LB414]
ATGAGCGTAGGTTTATTGCGGGTCTTGGTGCAACAAAAATTAGTCCCACAAGATAAAATTGAAAAATATCAAACAGCTAT
CAAAGAAAACAAACCTGTTTTGCCTTTGGTATTTGGCGATAAAATTATTTCGCCTGAAGCATTGGCAGAATTGTGTTCGT
CTTTGTTTAACTATCCGATGTTGGATTTGAACTATTTTCCACGTAGCGCGATTGTGCAAGATTTAATTGACGAAGCACAA
ATGCAAGAATTGCGTGCCATTCCTGTGTTAAAACGTGGGCGCAAAGTATTCTTTGCCGTTTCCGACCCTACGCAAATTCA
AAACGTACAAAAAGCGGTATTTGCCAGTGGTTTGCAAGTGGATTTGGTCATTGCGCGTGATGACCAATTAACTACTGTGT
TGGAATGGTTTGGTCAAAGTAACTCTGCTTTGCTCAAAGAAATGGGCATGGAGGTAAATAAAGAAGAAGCCAAACAGACC
ATGATTGTGGATGGACAGGAAGAGGAAGATGGTCCTGTCGCACGGTTTATTCAAAAAATCTTGCATGATGCGGTTACTTC
TGGTGCATCTGATATTCACTTTGAATTCTACGAATTTATGGCGCGTGTGCGTTTTCGTACCGATGGTGTGTTGCGCGAAG
TGGTGCAACCGCCCTTGCAACTGCGTAGCCAGTTGGCATCGCGTATTAAGGTGATGGCGAAATTGGATATTTCTGAAAAA
CGTGTACCGCAGGACGGACGTATTCAGTTGCAATTTTCTAAAAATACCAAAGCGATTGACTTTCGTGTGAACACCATGCC
GTGTTTGTTTGGTGAAAAAGTCGTGATGCGTATTTTGAACTCAGATGCAGCCAGCCTGAATATTGACCAGTTGGGTTTTG
AACCTTTCCAAAAAGAATTAATTATGGAAGCGATTCATCGTCCATACGGCATGGTGTTGGTAACAGGTCCAACGGGTTCG
GGTAAAACGGTTTCTCTCTATACTTGTTTGAACATTCTGAATACCGATGACATCAATATTTCTACTGCCGAAGACCCTGC
GGAGATTAACTTACCAGGGATTAACCAAGTAAACGTAAACGATAAACAAGGTTTAACTTTTGAGGCAGCCTTAAAAGCAT
TCCTGCGTCAAGACCCTGATGTGATTATGATTGGTGAGATTCGCGACTTGGGTACGGCGGATATTGCGATTAAAGCCGCA
CAAACAGGTCATATGGTATTCTCAACCTTGCACACCAATAATGCACCAGCTACTTTATCGCGTATGTTGAATATGGGTGT
TGCCCCATTTAACATTGCTTCGGCGGTAAACTTGATTATGGCACAACGTTTGGCGCGTCGTTTATGTTCTGCTTGCAAAG
CACCGATGGAGCGTCCACCTGAAAAAGCTTTATTAGAAGTAGGCTTTACTGAGGAGGATTTAGCGCAGGATTGGACAATG
TATCGCCCTGTGGGTTGTGATGCTTGTAAAGGTAAAGGCTATAAAGGTCGTGCAGGTTTGTATGAAGTAATGAAAATGTC
TGAAAAAATGAAAGCTGTCATCATGAGTGGCGGTACGGAGGTAGATATTGCTAATATCGCATATGAAGATGGTTTGGTAG
ATTTGCGTCGTTCAGGTTTACTGAAAGTTATGCAAGGTATTACTTCTTTGGAAGAAGTTATTGCAACCACAAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-140)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.231

100

0.692
  pilB Acinetobacter baumannii D1279779

53.427

100

0.544
  pilB Acinetobacter baylyi ADP1

52.465

100

0.533
  pilB Legionella pneumophila strain ERS1305867

47.679

100

0.478
  pilB Vibrio cholerae strain A1552

46.372

100

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

46.113

100

0.467
  pilB Vibrio campbellii strain DS40M4

47.793

93.202

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.652

92.487

0.367