Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   MIS44_RS04770 Genome accession   NZ_CP092357
Coordinates   892776..894062 (+) Length   428 a.a.
NCBI ID   WP_249446593.1    Uniprot ID   -
Organism   Wielerella bovis strain 10LB456     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 887776..899062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS44_RS04750 (MIS44_04750) lolA 888478..889059 (-) 582 WP_249449584.1 outer membrane lipoprotein chaperone LolA -
  MIS44_RS04755 (MIS44_04755) - 889256..890077 (+) 822 WP_249449585.1 ferritin-like domain-containing protein -
  MIS44_RS04760 (MIS44_04760) - 890074..890454 (+) 381 WP_249449353.1 CrcB family protein -
  MIS44_RS04765 (MIS44_04765) pilF 891072..892751 (+) 1680 WP_249449354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MIS44_RS04770 (MIS44_04770) pilG 892776..894062 (+) 1287 WP_249446593.1 type II secretion system F family protein Machinery gene
  MIS44_RS04775 (MIS44_04775) pilD 894072..894971 (+) 900 WP_249442250.1 A24 family peptidase Machinery gene
  MIS44_RS04780 (MIS44_04780) coaE 894975..895589 (+) 615 WP_249449355.1 dephospho-CoA kinase -
  MIS44_RS04785 (MIS44_04785) zapD 895602..896348 (+) 747 WP_249442248.1 cell division protein ZapD -
  MIS44_RS04790 (MIS44_04790) pncC 896401..896880 (+) 480 WP_249449356.1 nicotinamide-nucleotide amidase -
  MIS44_RS04795 (MIS44_04795) - 896955..897575 (+) 621 WP_249449357.1 L-threonylcarbamoyladenylate synthase -
  MIS44_RS04800 (MIS44_04800) murB 897591..898622 (+) 1032 WP_249449358.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 428 a.a.        Molecular weight: 46912.23 Da        Isoelectric Point: 9.9117

>NTDB_id=656670 MIS44_RS04770 WP_249446593.1 892776..894062(+) (pilG) [Wielerella bovis strain 10LB456]
MAEARKSAKSAAKKPVKKTAAKSAKPEKKEKGNRYQFEGKNLQTENIVRGEVVAKNEDEARQKLARRSIKVMQLTKMKKA
RAKKITQADITIFTRQLSTMMKAGLPMMQAFDIVAKGNPNANMTNLLLDIRNRVEQGESLGDSFAAHPAYFDKFYCNLVA
AGEAGGVLEGLLDKLATYKEKTEAIKKKVKGALTYPIAVMIVAIALILVMMMFVLPSFKKIYEGLGAELPGLTQWMMNAS
DFFVEKGWIIIPGLILGTWGLIQFHKHSFAFQKRVDAWLLKMPIFGEIVMKATIARWSRTTATLFTAGVPLVEVLDSVAG
AAGNIIYEEATHEIRSKVNQGISLTSGMQGANVFPNMVVQMASIGEESGALDDMLNKVAEFYEEEVDNAVATLSSLMEPI
IMVVLGSIVGVILVAMYLPLFSMGDAIG

Nucleotide


Download         Length: 1287 bp        

>NTDB_id=656670 MIS44_RS04770 WP_249446593.1 892776..894062(+) (pilG) [Wielerella bovis strain 10LB456]
ATGGCAGAAGCAAGAAAATCGGCAAAATCCGCCGCGAAAAAGCCAGTGAAAAAAACTGCAGCAAAATCAGCAAAACCTGA
AAAGAAAGAAAAAGGTAACCGCTATCAATTTGAAGGTAAGAACTTACAGACTGAAAATATTGTTCGCGGTGAGGTGGTTG
CAAAAAACGAAGATGAGGCACGTCAGAAGTTAGCGCGTCGCTCCATTAAAGTGATGCAATTAACCAAAATGAAAAAGGCG
CGTGCGAAGAAAATCACACAAGCTGATATTACCATCTTTACTCGCCAACTTTCTACTATGATGAAAGCGGGTTTGCCTAT
GATGCAAGCATTTGATATTGTGGCAAAAGGTAATCCAAATGCGAATATGACTAATTTATTGTTGGATATTCGTAATCGAG
TGGAGCAAGGTGAATCTTTGGGTGATTCTTTTGCTGCACATCCTGCTTATTTTGACAAATTCTATTGCAATTTGGTTGCA
GCAGGTGAAGCAGGTGGTGTGTTGGAGGGTTTGCTGGATAAATTGGCAACCTATAAAGAAAAAACGGAAGCTATTAAAAA
GAAAGTAAAAGGTGCTTTAACGTATCCGATTGCGGTGATGATAGTCGCAATTGCTTTGATTTTGGTTATGATGATGTTCG
TGTTGCCATCATTTAAGAAAATTTATGAAGGTTTGGGAGCAGAGCTGCCAGGTTTAACACAATGGATGATGAATGCGTCT
GATTTCTTTGTAGAAAAAGGTTGGATTATTATTCCTGGATTAATTTTGGGTACATGGGGCTTAATTCAATTCCATAAACA
TTCTTTTGCTTTCCAAAAACGGGTAGATGCTTGGTTATTAAAAATGCCTATTTTTGGCGAGATTGTGATGAAAGCAACCA
TTGCACGTTGGTCGCGTACAACTGCAACTTTGTTTACTGCTGGTGTGCCTTTAGTAGAGGTATTGGATTCGGTAGCAGGT
GCTGCTGGTAATATTATTTATGAGGAAGCTACTCATGAAATTCGCAGTAAAGTAAACCAAGGCATTTCTTTGACCTCAGG
TATGCAAGGTGCGAATGTCTTCCCTAATATGGTGGTACAAATGGCATCTATTGGTGAAGAATCAGGTGCTCTGGACGATA
TGTTGAACAAAGTAGCAGAATTCTATGAGGAAGAAGTAGATAATGCTGTTGCCACTTTATCTTCGTTGATGGAACCAATT
ATTATGGTGGTATTAGGTAGTATCGTTGGTGTGATTCTGGTTGCGATGTACTTGCCACTGTTCAGCATGGGCGATGCCAT
CGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(93-216)

Type II secretion system (T2SS), protein F (T2SSF)

(297-419)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

63.25

93.458

0.591
  pilG Neisseria meningitidis 44/76-A

62.5

93.458

0.584
  pilC Legionella pneumophila strain ERS1305867

47.487

92.991

0.442
  pilC Pseudomonas stutzeri DSM 10701

43.812

94.393

0.414
  pilC Acinetobacter baumannii D1279779

45.455

89.953

0.409
  pilC Acinetobacter baylyi ADP1

44.444

90.421

0.402