Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MIS44_RS04765 Genome accession   NZ_CP092357
Coordinates   891072..892751 (+) Length   559 a.a.
NCBI ID   WP_249449354.1    Uniprot ID   -
Organism   Wielerella bovis strain 10LB456     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 886072..897751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS44_RS04735 (MIS44_04735) - 886130..886642 (+) 513 WP_249449351.1 protein disulfide oxidoreductase -
  MIS44_RS04740 (MIS44_04740) rbfA 886697..887068 (-) 372 WP_249449352.1 30S ribosome-binding factor RbfA -
  MIS44_RS04745 (MIS44_04745) - 887311..888414 (-) 1104 WP_249449583.1 anhydro-N-acetylmuramic acid kinase -
  MIS44_RS04750 (MIS44_04750) lolA 888478..889059 (-) 582 WP_249449584.1 outer membrane lipoprotein chaperone LolA -
  MIS44_RS04755 (MIS44_04755) - 889256..890077 (+) 822 WP_249449585.1 ferritin-like domain-containing protein -
  MIS44_RS04760 (MIS44_04760) - 890074..890454 (+) 381 WP_249449353.1 CrcB family protein -
  MIS44_RS04765 (MIS44_04765) pilF 891072..892751 (+) 1680 WP_249449354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MIS44_RS04770 (MIS44_04770) pilG 892776..894062 (+) 1287 WP_249446593.1 type II secretion system F family protein Machinery gene
  MIS44_RS04775 (MIS44_04775) pilD 894072..894971 (+) 900 WP_249442250.1 A24 family peptidase Machinery gene
  MIS44_RS04780 (MIS44_04780) coaE 894975..895589 (+) 615 WP_249449355.1 dephospho-CoA kinase -
  MIS44_RS04785 (MIS44_04785) zapD 895602..896348 (+) 747 WP_249442248.1 cell division protein ZapD -
  MIS44_RS04790 (MIS44_04790) pncC 896401..896880 (+) 480 WP_249449356.1 nicotinamide-nucleotide amidase -
  MIS44_RS04795 (MIS44_04795) - 896955..897575 (+) 621 WP_249449357.1 L-threonylcarbamoyladenylate synthase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62065.89 Da        Isoelectric Point: 5.2149

>NTDB_id=656669 MIS44_RS04765 WP_249449354.1 891072..892751(+) (pilF) [Wielerella bovis strain 10LB456]
MSVGLLRVLVQQKLVPQDKIEKYQTAIKENKPVLPLVFGDKIISPEALAELCSSLFNYPMLDLNYFPRSAIVQDLIDETQ
MQELRAIPVLKRGRKVFFAVSDPTQIQNVQKAVFASGLQVDLVIARDDQLTTVLEWFGQSNSALLKEMGMEVNKEEAKQT
MIVDGQEEEDGPVARFIQKILHDAVTSGASDIHFEFYEFMARVRFRTDGVLREVVQPPLQLRSQLASRIKVMAKLDISEK
RVPQDGRIQLQFSKNTKAIDFRVNTMPCLFGEKVVMRILNSDAASLNIDQLGFEPFQKELIMEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTDDINISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDVIMIGEIRDLGTADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCSACKAPMERPPEKALLEVGFTEEDLAQDWTM
YRPVGCDACKGKGYKGRAGLYEVMKMSEKMKAVIMSGGTEVDIANIAYEDGLVDLRRSGLLKVMQGITSLEEVIATTNE

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=656669 MIS44_RS04765 WP_249449354.1 891072..892751(+) (pilF) [Wielerella bovis strain 10LB456]
ATGAGCGTAGGTTTATTGCGGGTCTTGGTGCAACAAAAATTAGTCCCACAAGATAAAATTGAAAAATATCAAACGGCTAT
CAAAGAAAACAAACCTGTTTTACCTTTGGTGTTTGGCGATAAAATTATTTCGCCTGAAGCATTGGCAGAATTGTGTTCGT
CTTTATTCAACTATCCGATGTTGGATTTGAACTATTTTCCACGCAGCGCGATTGTGCAAGATTTGATTGATGAAACACAA
ATGCAAGAATTGCGTGCGATTCCTGTGTTAAAACGTGGTCGAAAAGTGTTCTTCGCTGTTTCAGACCCTACGCAAATTCA
AAACGTACAAAAAGCGGTATTTGCCAGCGGTTTGCAAGTGGATTTGGTCATCGCGCGTGATGACCAATTAACTACTGTGT
TGGAATGGTTTGGTCAAAGTAACTCTGCTTTGCTCAAAGAAATGGGCATGGAGGTGAATAAAGAAGAAGCCAAGCAAACC
ATGATTGTGGATGGACAGGAAGAAGAAGATGGTCCTGTCGCACGGTTTATTCAAAAAATCTTGCATGATGCGGTTACTTC
TGGTGCATCTGATATTCACTTTGAATTCTACGAATTTATGGCGCGTGTGCGTTTTCGTACCGATGGTGTGTTGCGCGAAG
TGGTGCAACCGCCCTTGCAACTGCGTAGCCAGTTGGCATCGCGTATTAAAGTGATGGCGAAATTGGATATTTCTGAAAAA
CGTGTACCGCAGGACGGACGTATTCAGTTGCAATTTTCTAAAAATACTAAAGCGATTGACTTTCGTGTGAATACCATGCC
GTGTTTGTTTGGCGAAAAAGTCGTGATGCGTATTTTGAACTCGGACGCAGCCAGCCTGAATATTGACCAGTTGGGTTTTG
AACCTTTCCAAAAAGAATTAATTATGGAAGCGATTCATCGTCCATACGGCATGGTGTTGGTAACAGGTCCAACGGGTTCG
GGTAAAACGGTTTCGCTCTATACTTGCTTGAACATTCTGAATACCGATGATATTAATATTTCTACCGCCGAAGACCCTGC
GGAGATTAACTTGCCGGGGATTAACCAAGTAAACGTAAATGATAAACAAGGTTTAACTTTTGAGGCAGCCTTAAAAGCAT
TCCTGCGTCAAGACCCTGATGTAATTATGATTGGTGAGATTCGTGACTTGGGTACGGCGGATATTGCAATTAAAGCAGCG
CAAACAGGTCACATGGTATTCTCAACCTTGCACACCAATAATGCACCAGCTACTTTGTCGCGTATGTTAAATATGGGTGT
AGCACCATTTAATATTGCTTCGGCGGTAAACTTGATTATGGCACAACGTTTGGCGCGTCGTTTATGTTCTGCTTGCAAAG
CACCGATGGAGCGTCCACCTGAAAAAGCTTTATTAGAAGTAGGCTTTACTGAGGAGGATTTAGCGCAGGATTGGACAATG
TATCGCCCTGTGGGTTGTGATGCTTGTAAAGGTAAAGGCTATAAAGGTCGTGCAGGTTTGTATGAAGTAATGAAAATGTC
TGAAAAAATGAAAGCTGTCATCATGAGTGGCGGTACGGAGGTGGATATTGCTAATATCGCATATGAAGATGGTTTGGTAG
ATTTGCGTCGTTCAGGTTTACTGAAAGTTATGCAAGGTATTACTTCTTTGGAAGAAGTTATTGCAACCACAAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-140)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.231

100

0.692
  pilB Acinetobacter baumannii D1279779

53.521

100

0.544
  pilB Acinetobacter baylyi ADP1

52.465

100

0.533
  pilB Legionella pneumophila strain ERS1305867

47.679

100

0.478
  pilB Vibrio cholerae strain A1552

46.372

100

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

46.113

100

0.467
  pilB Vibrio campbellii strain DS40M4

47.793

93.202

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.458

92.487

0.365