Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L3Q72_RS02820 Genome accession   NZ_CP092106
Coordinates   592705..594381 (-) Length   558 a.a.
NCBI ID   WP_275131164.1    Uniprot ID   -
Organism   Vibrio sp. JC009     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 587705..599381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3Q72_RS02795 (L3Q72_02790) yaaA 588265..589038 (+) 774 WP_275131159.1 peroxide stress protein YaaA -
  L3Q72_RS02800 (L3Q72_02795) zapD 589172..589912 (-) 741 WP_275131160.1 cell division protein ZapD -
  L3Q72_RS02805 (L3Q72_02800) coaE 589959..590558 (-) 600 WP_275131161.1 dephospho-CoA kinase -
  L3Q72_RS02810 (L3Q72_02805) pilD 590562..591431 (-) 870 WP_275131162.1 A24 family peptidase Machinery gene
  L3Q72_RS02815 (L3Q72_02810) pilC 591474..592730 (-) 1257 WP_275131163.1 type II secretion system F family protein Machinery gene
  L3Q72_RS02820 (L3Q72_02815) pilB 592705..594381 (-) 1677 WP_275131164.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L3Q72_RS02825 (L3Q72_02820) - 594382..594837 (-) 456 WP_275131165.1 pilin -
  L3Q72_RS02830 (L3Q72_02830) - 595197..596270 (+) 1074 WP_275131166.1 hypothetical protein -
  L3Q72_RS02835 (L3Q72_02835) - 596292..596843 (+) 552 WP_275131167.1 outer membrane beta-barrel protein -
  L3Q72_RS02840 (L3Q72_02840) - 596920..597471 (+) 552 WP_275131168.1 hypothetical protein -
  L3Q72_RS02845 (L3Q72_02845) nadC 597531..598418 (-) 888 WP_275131169.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L3Q72_RS02850 (L3Q72_02850) ampD 598764..599309 (+) 546 WP_275131170.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61857.60 Da        Isoelectric Point: 6.6127

>NTDB_id=655900 L3Q72_RS02820 WP_275131164.1 592705..594381(-) (pilB) [Vibrio sp. JC009]
MSSLISILCQHQLLTPEQSSEARTLCEKETIPAVVALNKLGYFTFDQLSAHISSLFSMPLTDIRAYNYEPVCHQMGLRKL
ICKHSALPVELNGNELLVAVSDPCNSQAEDDFRFATRYQIRLCLADYKSISGAIRHLYGQTDNSEELTGREIHEDELSGL
VEASDETESSTTAEEDQAPVSRFISQVIEDAVRKGASDIHFEPYENHYRIRFRCDGILIETQKPAPQLARRLAARIKILS
KLDIAERRIPQDGRIKLKTGRGNLVDLRVSTLPTLWGEKVVLRILDSHQASLDIKQLGFSDKQIALYSEALKKPQGMILI
TGPTGSGKTVSLYTGLNILNSPELNISTAEDPVEINLPGINQVQINRKIHFDFAQALRAFLRQDPDVVMVGEIRDSETAA
IAIRAAQTGHLVLSTLHTNSAIEAVTRLGNLGTENFDIASSLSLVIAQRLVRRLCPHCKQKQNFPQELLKELSISKHTVL
YQAKKEGCTECNGGYSGRVGIYEVINCDSQLRSLIAQGASYEQLETVAISSGMKTLKESGLDKLKQGATSYAEIQRVL

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=655900 L3Q72_RS02820 WP_275131164.1 592705..594381(-) (pilB) [Vibrio sp. JC009]
ATGAGCTCTCTTATCTCTATCTTATGCCAGCATCAGCTTTTAACCCCGGAGCAAAGCTCAGAAGCCCGCACGCTATGTGA
AAAAGAAACGATCCCTGCTGTCGTTGCTCTGAATAAGCTTGGCTACTTTACCTTTGACCAGCTCTCCGCTCATATCAGCT
CTCTGTTTTCCATGCCACTGACTGACATCCGGGCCTATAACTATGAACCTGTCTGTCATCAGATGGGGCTAAGAAAGCTA
ATCTGCAAACATTCCGCTTTGCCGGTAGAGCTGAACGGTAACGAGCTGTTAGTTGCGGTCAGTGATCCCTGCAACTCCCA
GGCAGAAGATGATTTCCGCTTTGCTACCCGTTACCAGATAAGGCTTTGCCTTGCCGATTACAAATCCATATCAGGCGCTA
TCCGGCATCTTTACGGACAAACGGATAACAGCGAAGAGCTCACCGGCAGGGAGATCCATGAAGACGAACTGAGCGGGCTT
GTAGAGGCTTCAGATGAAACAGAAAGCAGCACAACCGCTGAAGAAGATCAGGCTCCCGTCAGCCGCTTTATCAGCCAGGT
CATTGAAGATGCCGTACGAAAAGGCGCTTCAGATATTCACTTCGAACCCTATGAAAACCACTACCGGATCCGCTTTCGCT
GCGACGGAATTTTAATAGAAACTCAGAAACCGGCCCCTCAGCTTGCCCGCCGGTTAGCTGCTCGAATTAAAATCCTGTCA
AAACTGGATATCGCAGAAAGACGGATTCCTCAGGATGGCAGGATTAAGTTAAAAACCGGCAGAGGAAATCTGGTTGACCT
CAGAGTGTCCACCCTGCCAACGTTGTGGGGAGAAAAAGTGGTTCTGCGTATTCTGGACAGTCATCAGGCCAGCCTGGATA
TCAAGCAGCTTGGCTTCAGTGACAAACAGATTGCACTCTACTCAGAGGCGCTAAAAAAGCCGCAGGGAATGATATTAATA
ACAGGGCCGACGGGAAGCGGAAAAACCGTCTCCCTCTATACGGGGCTTAACATTCTGAACAGCCCGGAGCTAAATATTTC
AACCGCTGAAGACCCGGTTGAGATTAACCTTCCCGGCATTAATCAGGTTCAGATAAACCGGAAAATTCACTTTGACTTTG
CTCAGGCTCTCCGGGCTTTCCTGCGTCAGGATCCGGATGTGGTTATGGTTGGGGAAATCCGTGACTCCGAAACGGCGGCT
ATTGCCATCAGAGCCGCTCAGACCGGTCACCTTGTTCTTTCCACCCTGCATACCAATTCAGCCATTGAAGCCGTGACCCG
TCTTGGTAATTTAGGCACAGAGAATTTCGATATCGCCTCATCCTTAAGTCTGGTTATTGCACAAAGACTTGTCAGACGGC
TTTGCCCTCACTGCAAGCAAAAACAAAACTTCCCACAGGAGCTTCTAAAGGAACTGAGCATCAGTAAACATACGGTTCTT
TATCAGGCTAAAAAAGAGGGCTGCACCGAATGCAACGGCGGTTACTCCGGGCGAGTCGGGATCTATGAAGTAATAAACTG
CGACTCGCAGCTCAGAAGCCTGATTGCCCAGGGAGCCAGTTATGAACAACTGGAAACAGTGGCAATAAGCAGCGGGATGA
AAACGTTAAAAGAGTCAGGCCTCGATAAGCTAAAACAGGGAGCCACCAGCTATGCCGAAATACAGCGGGTTCTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-142)

Type II/IV secretion system protein (T2SSE)

(186-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

59.821

100

0.6
  pilB Vibrio parahaemolyticus RIMD 2210633

59.319

100

0.593
  pilB Vibrio campbellii strain DS40M4

58.497

100

0.586
  pilF Neisseria gonorrhoeae MS11

45.098

100

0.453
  pilB Legionella pneumophila strain ERS1305867

44.602

100

0.452
  pilB Acinetobacter baumannii D1279779

48.155

92.294

0.444
  pilB Acinetobacter baylyi ADP1

47.776

92.652

0.443
  pilF Thermus thermophilus HB27

39.583

94.624

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

37.226

98.208

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.38

85.842

0.364