Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   LVJ80_RS08695 Genome accession   NZ_CP091519
Coordinates   1648553..1649083 (+) Length   176 a.a.
NCBI ID   WP_034296617.1    Uniprot ID   A0A376BLS2
Organism   Alysiella crassa strain S6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1643553..1654083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ80_RS08680 (LVJ80_01290) - 1644424..1646838 (-) 2415 WP_051968676.1 dynamin family protein -
  LVJ80_RS08685 (LVJ80_01285) ampD 1646886..1647443 (-) 558 WP_034296620.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LVJ80_RS08690 (LVJ80_01280) - 1647549..1648220 (+) 672 WP_244773280.1 YdcF family protein -
  LVJ80_RS08695 (LVJ80_01275) pilE 1648553..1649083 (+) 531 WP_034296617.1 pilin Machinery gene
  LVJ80_RS08700 (LVJ80_01265) pilE 1649222..1649746 (+) 525 WP_034296615.1 pilin Machinery gene
  LVJ80_RS08705 (LVJ80_01260) ubiM 1649817..1650995 (-) 1179 WP_034296613.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  LVJ80_RS08710 (LVJ80_01255) ribF 1651188..1652123 (+) 936 WP_034296611.1 bifunctional riboflavin kinase/FAD synthetase -
  LVJ80_RS08715 (LVJ80_01250) - 1652311..1652967 (-) 657 WP_034296609.1 trimeric intracellular cation channel family protein -
  LVJ80_RS08720 (LVJ80_01245) - 1652999..1653826 (-) 828 WP_034296606.1 YoaK family protein -

Sequence

Protein


Download         Length: 176 a.a.        Molecular weight: 18084.74 Da        Isoelectric Point: 8.4400

>NTDB_id=652010 LVJ80_RS08695 WP_034296617.1 1648553..1649083(+) (pilE) [Alysiella crassa strain S6]
MKTMQKGFTLIELMIVIAIIGILAAIALPAYQDYTVRSKISEGLIASSAAKGMVSEAFQSDSVAGIVSAAAAYNAKPDAE
KSSKYVKTIKISEDDKSAGVITVTLSESDAVGFPGEVKGKTIVLSPNVNNEAPAQGAAGAIDWACASAAGTTATKRGLKN
VQTGNLPAKYAPSECR

Nucleotide


Download         Length: 531 bp        

>NTDB_id=652010 LVJ80_RS08695 WP_034296617.1 1648553..1649083(+) (pilE) [Alysiella crassa strain S6]
ATGAAAACGATGCAAAAAGGTTTCACATTAATCGAGTTGATGATTGTAATCGCAATTATCGGTATTTTGGCAGCTATCGC
ATTGCCTGCTTACCAAGACTACACTGTACGTTCTAAAATCTCTGAAGGTTTGATTGCTTCTTCTGCTGCTAAAGGCATGG
TTTCTGAAGCATTCCAATCTGACAGCGTAGCTGGTATCGTATCTGCAGCTGCTGCGTACAACGCTAAACCAGATGCTGAA
AAATCTTCTAAATATGTTAAAACTATTAAAATTAGTGAAGATGACAAAAGTGCTGGTGTGATTACTGTAACTTTATCAGA
ATCTGATGCTGTAGGTTTTCCTGGTGAAGTTAAAGGTAAAACAATTGTCCTCTCACCAAACGTAAATAATGAGGCTCCTG
CACAAGGTGCAGCTGGTGCAATTGACTGGGCTTGTGCTTCTGCTGCTGGTACTACAGCTACTAAACGTGGTTTGAAAAAT
GTACAAACAGGTAATTTGCCAGCTAAATATGCTCCTTCTGAGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-176)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A376BLS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

56.842

100

0.614
  pilA Ralstonia pseudosolanacearum GMI1000

40.957

100

0.437
  comP Acinetobacter baylyi ADP1

41.989

100

0.432
  pilE Neisseria gonorrhoeae MS11

36.957

100

0.386
  pilA2 Legionella pneumophila str. Paris

39.535

97.727

0.386
  pilA2 Legionella pneumophila strain ERS1305867

38.953

97.727

0.381
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.209

97.727

0.364