Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   LVJ80_RS08700 Genome accession   NZ_CP091519
Coordinates   1649222..1649746 (+) Length   174 a.a.
NCBI ID   WP_034296615.1    Uniprot ID   A0A376BM40
Organism   Alysiella crassa strain S6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1644222..1654746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ80_RS08680 (LVJ80_01290) - 1644424..1646838 (-) 2415 WP_051968676.1 dynamin family protein -
  LVJ80_RS08685 (LVJ80_01285) ampD 1646886..1647443 (-) 558 WP_034296620.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LVJ80_RS08690 (LVJ80_01280) - 1647549..1648220 (+) 672 WP_244773280.1 YdcF family protein -
  LVJ80_RS08695 (LVJ80_01275) pilE 1648553..1649083 (+) 531 WP_034296617.1 pilin Machinery gene
  LVJ80_RS08700 (LVJ80_01265) pilE 1649222..1649746 (+) 525 WP_034296615.1 pilin Machinery gene
  LVJ80_RS08705 (LVJ80_01260) ubiM 1649817..1650995 (-) 1179 WP_034296613.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  LVJ80_RS08710 (LVJ80_01255) ribF 1651188..1652123 (+) 936 WP_034296611.1 bifunctional riboflavin kinase/FAD synthetase -
  LVJ80_RS08715 (LVJ80_01250) - 1652311..1652967 (-) 657 WP_034296609.1 trimeric intracellular cation channel family protein -
  LVJ80_RS08720 (LVJ80_01245) - 1652999..1653826 (-) 828 WP_034296606.1 YoaK family protein -
  LVJ80_RS08725 (LVJ80_01240) - 1653886..1654344 (-) 459 WP_034296603.1 low molecular weight protein-tyrosine-phosphatase -

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 18698.56 Da        Isoelectric Point: 8.4272

>NTDB_id=652011 LVJ80_RS08700 WP_034296615.1 1649222..1649746(+) (pilE) [Alysiella crassa strain S6]
MEKKLQQGFTLIELMIVIAIIGILAAIALPAYQDYTVRAKIGEALLAGSAPKNYLAEAFQADGYTGVVNASKSYNVKPKA
EKQSKYVEDIQINETTGEIAVFLRSDIDVGFPKDVQGKTLILTPHIHGDKLVENKWGAIDWACSSTSTAAAANRGLKAAV
KGTLPAKYAPSECR

Nucleotide


Download         Length: 525 bp        

>NTDB_id=652011 LVJ80_RS08700 WP_034296615.1 1649222..1649746(+) (pilE) [Alysiella crassa strain S6]
ATGGAAAAAAAATTACAACAAGGTTTTACATTAATTGAATTGATGATTGTGATTGCAATCATTGGTATTTTAGCAGCCAT
TGCGTTGCCAGCTTATCAGGATTATACGGTGAGAGCAAAAATAGGGGAGGCGTTGTTGGCTGGGTCTGCGCCTAAAAATT
ATTTGGCTGAAGCTTTTCAGGCTGATGGTTATACTGGCGTTGTAAATGCTTCTAAAAGCTACAATGTCAAACCGAAAGCC
GAAAAACAATCCAAATATGTAGAGGATATTCAAATTAACGAAACGACTGGTGAAATTGCAGTTTTTTTGCGTAGCGATAT
AGATGTGGGTTTCCCAAAAGATGTGCAAGGTAAGACTTTGATTTTAACGCCACATATTCACGGCGATAAATTGGTGGAGA
ATAAGTGGGGTGCAATTGATTGGGCGTGTAGTTCCACTTCTACGGCTGCTGCGGCTAATCGTGGTTTGAAAGCGGCTGTA
AAAGGAACATTACCCGCCAAATATGCACCATCTGAATGCCGTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)

Pilin (bacterial filament) (Pilin)

(37-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A376BM40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

48.404

100

0.523
  pilA Ralstonia pseudosolanacearum GMI1000

39.888

100

0.408
  pilE Neisseria gonorrhoeae MS11

37.059

97.701

0.362
  comP Acinetobacter baylyi ADP1

35.795

100

0.362