Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   LVJ79_RS08045 Genome accession   NZ_CP091518
Coordinates   1504415..1505683 (-) Length   422 a.a.
NCBI ID   WP_003787779.1    Uniprot ID   A0AAX2J5Z2
Organism   Kingella kingae strain 4177/66     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1499415..1510683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ79_RS08015 (LVJ79_08015) - 1499621..1500814 (+) 1194 WP_003787791.1 acetate kinase -
  LVJ79_RS08020 (LVJ79_08020) - 1500889..1501509 (-) 621 WP_003787790.1 L-threonylcarbamoyladenylate synthase -
  LVJ79_RS08025 (LVJ79_08025) pncC 1501519..1501995 (-) 477 WP_003787789.1 nicotinamide-nucleotide amidase -
  LVJ79_RS08030 (LVJ79_08030) zapD 1502015..1502776 (-) 762 WP_003787787.1 cell division protein ZapD -
  LVJ79_RS08035 (LVJ79_08035) coaE 1502891..1503499 (-) 609 WP_003787784.1 dephospho-CoA kinase -
  LVJ79_RS08040 (LVJ79_08040) pilD 1503501..1504403 (-) 903 WP_003787782.1 prepilin peptidase Machinery gene
  LVJ79_RS08045 (LVJ79_08045) pilG 1504415..1505683 (-) 1269 WP_003787779.1 type II secretion system F family protein Machinery gene
  LVJ79_RS08050 (LVJ79_08050) pilF 1505730..1507415 (-) 1686 WP_003787777.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ79_RS08055 (LVJ79_08055) lysA 1507601..1508827 (-) 1227 WP_003787776.1 diaminopimelate decarboxylase -
  LVJ79_RS08060 (LVJ79_08060) lptM 1508827..1508988 (-) 162 WP_003787775.1 LPS translocon maturation chaperone LptM -
  LVJ79_RS08065 (LVJ79_08065) cyaY 1509079..1509402 (+) 324 WP_099046165.1 iron donor protein CyaY -
  LVJ79_RS08070 (LVJ79_08070) pgeF 1509552..1510331 (+) 780 WP_003787769.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46018.37 Da        Isoelectric Point: 10.0411

>NTDB_id=651988 LVJ79_RS08045 WP_003787779.1 1504415..1505683(-) (pilG) [Kingella kingae strain 4177/66]
MAAKKPVKKASVKKAAAAKPVKKEKGNRYQFEGRNLSTDSIVRGEVIAKNEDEARQKLARRQIKVLQLTKMKKAKDKKIT
SADIAVFTRQLSTMMKAGLPLMQAFDIVAKGHANASMTAMLMEIRNDVEQGEALGDSFAKHPKYFDKFYCNLVAAGEAGG
VLEGLLDKLATYKEKTEAIKKKVKSALTYPISIVVVAVALVIVMMMFVLPEFGKVYSSMGAELPPLTQFMMNISNAFVEF
GWLIILALIGGVVALIQFHKRSHAFQKKVDTWLLKTPIFGDIVRKATIARWARTTATLFTAGVPLVEVLDSVAGAAGNII
YEEATREIRSKVNQGISLTSGMQATNLFPNMVVQMASIGEESGALDDMLNKTAEFYEDEVDAAVGMLSSLMEPIIMVVLG
GIIGVILVAMYLPLFNLGNVVA

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=651988 LVJ79_RS08045 WP_003787779.1 1504415..1505683(-) (pilG) [Kingella kingae strain 4177/66]
ATGGCTGCAAAAAAACCTGTTAAAAAAGCATCTGTAAAAAAAGCAGCGGCTGCCAAGCCTGTTAAGAAAGAAAAAGGCAA
TCGCTACCAGTTTGAAGGTCGCAATTTAAGTACCGATTCAATCGTACGTGGCGAAGTGATTGCTAAAAATGAAGACGAAG
CACGTCAGAAGTTAGCGCGTCGCCAAATTAAGGTGTTGCAACTGACTAAAATGAAAAAGGCAAAAGATAAGAAAATTACT
TCTGCCGACATTGCGGTGTTTACGCGCCAACTTTCTACCATGATGAAAGCAGGTTTACCATTGATGCAAGCGTTTGATAT
TGTGGCAAAAGGTCATGCCAATGCATCTATGACTGCCATGCTGATGGAAATTCGCAACGATGTGGAACAAGGTGAAGCAT
TGGGCGATTCGTTTGCTAAGCACCCTAAATATTTTGACAAATTCTATTGCAACTTGGTTGCCGCAGGTGAGGCAGGCGGT
GTATTGGAAGGCTTGTTAGACAAACTGGCAACCTATAAAGAAAAAACCGAAGCGATTAAGAAAAAAGTGAAATCGGCTTT
GACTTACCCAATTTCTATTGTGGTGGTGGCGGTTGCCTTGGTGATTGTGATGATGATGTTCGTGTTGCCAGAGTTTGGTA
AGGTGTACAGTAGCATGGGCGCAGAATTGCCACCGTTGACGCAATTTATGATGAATATTTCCAATGCGTTTGTGGAATTT
GGTTGGTTGATTATTTTGGCTTTGATTGGCGGTGTGGTTGCGCTGATTCAATTCCATAAGAGATCTCATGCGTTCCAGAA
AAAAGTGGATACATGGTTGTTAAAAACGCCAATTTTTGGTGATATTGTTCGTAAAGCAACGATTGCACGTTGGGCACGTA
CTACTGCAACCTTATTTACTGCGGGTGTGCCATTGGTGGAAGTATTGGATTCGGTAGCTGGTGCAGCAGGTAATATTATT
TACGAAGAAGCCACTCGCGAAATTCGTTCCAAAGTAAACCAAGGTATTTCTTTGACATCGGGTATGCAGGCAACTAATTT
ATTCCCGAACATGGTAGTGCAAATGGCTTCCATTGGTGAAGAATCTGGCGCGTTGGATGATATGTTAAACAAAACCGCTG
AATTCTATGAAGACGAAGTTGATGCTGCGGTGGGAATGTTGTCATCTTTGATGGAACCCATCATTATGGTGGTATTAGGC
GGTATCATTGGTGTGATTCTGGTCGCAATGTACTTGCCATTGTTTAACTTGGGTAATGTAGTGGCATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

68.08

95.024

0.647
  pilG Neisseria meningitidis 44/76-A

67.332

95.024

0.64
  pilC Legionella pneumophila strain ERS1305867

47.619

94.55

0.45
  pilC Acinetobacter baumannii D1279779

45.455

91.232

0.415
  pilC Acinetobacter baylyi ADP1

45.078

91.469

0.412
  pilC Pseudomonas stutzeri DSM 10701

49.565

81.754

0.405
  pilC Vibrio cholerae strain A1552

37.981

98.578

0.374