Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LVJ79_RS08050 Genome accession   NZ_CP091518
Coordinates   1505730..1507415 (-) Length   561 a.a.
NCBI ID   WP_003787777.1    Uniprot ID   F5S8J8
Organism   Kingella kingae strain 4177/66     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1500730..1512415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ79_RS08020 (LVJ79_08020) - 1500889..1501509 (-) 621 WP_003787790.1 L-threonylcarbamoyladenylate synthase -
  LVJ79_RS08025 (LVJ79_08025) pncC 1501519..1501995 (-) 477 WP_003787789.1 nicotinamide-nucleotide amidase -
  LVJ79_RS08030 (LVJ79_08030) zapD 1502015..1502776 (-) 762 WP_003787787.1 cell division protein ZapD -
  LVJ79_RS08035 (LVJ79_08035) coaE 1502891..1503499 (-) 609 WP_003787784.1 dephospho-CoA kinase -
  LVJ79_RS08040 (LVJ79_08040) pilD 1503501..1504403 (-) 903 WP_003787782.1 prepilin peptidase Machinery gene
  LVJ79_RS08045 (LVJ79_08045) pilG 1504415..1505683 (-) 1269 WP_003787779.1 type II secretion system F family protein Machinery gene
  LVJ79_RS08050 (LVJ79_08050) pilF 1505730..1507415 (-) 1686 WP_003787777.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ79_RS08055 (LVJ79_08055) lysA 1507601..1508827 (-) 1227 WP_003787776.1 diaminopimelate decarboxylase -
  LVJ79_RS08060 (LVJ79_08060) lptM 1508827..1508988 (-) 162 WP_003787775.1 LPS translocon maturation chaperone LptM -
  LVJ79_RS08065 (LVJ79_08065) cyaY 1509079..1509402 (+) 324 WP_099046165.1 iron donor protein CyaY -
  LVJ79_RS08070 (LVJ79_08070) pgeF 1509552..1510331 (+) 780 WP_003787769.1 peptidoglycan editing factor PgeF -
  LVJ79_RS08075 (LVJ79_08075) - 1510383..1512008 (-) 1626 WP_003787766.1 ABC-F family ATPase -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62226.13 Da        Isoelectric Point: 5.1186

>NTDB_id=651989 LVJ79_RS08050 WP_003787777.1 1505730..1507415(-) (pilF) [Kingella kingae strain 4177/66]
MSAGLLRILVQKKLVPQENLAKYQEAIKADKPILASIFADKLITPEDLAELCATMFNYPMLDLNFFPRSKIVPDILDESQ
MMELRCIPLFKRGKKLYLAVSDPTNIQNLQKVVFSSGLAIDLVIVRDDQLNTVLEWFGQSNSSLLKEMGMDSSLQEAQAQ
TGMIVDGEEEEDGPVARFIQKILHDAVTSGASDIHFEFYEFMARVRFRTDGQLREIVQPPLALRSQLASRIKVMAKLDIS
EKRVPQDGRIQLQFSKNTKAIDFRVNTMPCLFGEKVVMRILNSDAASLNIDQLGFEDFQKKLIMDAIYRPYGMVLVTGPT
GSGKTVSLYTCLNILNTDDVNISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDIIMIGEIRDLGTADIAIK
AAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCSACKAPMERPPENVLRDAGFTDEDLAKDW
TMYRAVGCDACKGKGYKGRAGLYEVMPMTEKMKAVIMKGGTEVDIANIAYEEGLCDLRRSGLLKVMQGVTTLEEVIATTN
E

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=651989 LVJ79_RS08050 WP_003787777.1 1505730..1507415(-) (pilF) [Kingella kingae strain 4177/66]
ATGAGCGCAGGTTTATTGCGAATTTTGGTACAGAAAAAACTCGTACCACAAGAGAATTTAGCCAAATATCAAGAAGCGAT
TAAAGCCGATAAACCGATTTTGGCTTCCATTTTTGCCGACAAGCTGATTACACCAGAAGACTTGGCAGAGCTGTGTGCCA
CCATGTTTAACTATCCGATGTTGGATTTAAACTTCTTCCCACGCAGCAAAATCGTCCCTGATATTTTGGATGAATCGCAA
ATGATGGAATTGCGCTGCATTCCATTGTTTAAGCGTGGTAAAAAATTGTATTTGGCGGTGTCTGACCCAACCAATATCCA
AAATTTGCAAAAAGTCGTGTTCAGCAGCGGCTTGGCGATTGATTTAGTGATTGTGCGCGATGATCAATTAAATACCGTGT
TGGAATGGTTTGGTCAAAGCAATAGCAGCCTGCTTAAAGAAATGGGCATGGACAGCAGCCTGCAAGAAGCACAAGCCCAA
ACAGGCATGATTGTGGATGGCGAAGAAGAAGAGGACGGCCCTGTTGCCCGTTTCATTCAAAAAATTCTGCACGATGCCGT
GACATCGGGTGCGTCCGATATTCACTTTGAGTTCTATGAATTTATGGCGCGTGTGCGTTTCCGTACAGACGGTCAGTTGC
GCGAAATCGTACAGCCACCATTGGCATTGCGTAGCCAGCTGGCATCGCGCATCAAGGTAATGGCGAAACTGGATATTTCT
GAAAAGCGCGTCCCACAAGACGGACGTATTCAGTTGCAATTTTCTAAAAATACCAAAGCGATTGACTTTCGTGTGAACAC
CATGCCGTGTTTGTTTGGCGAAAAAGTCGTGATGCGTATTTTGAACTCCGATGCCGCCAGCTTGAACATTGACCAACTGG
GTTTTGAAGACTTCCAGAAAAAATTGATTATGGACGCGATTTATCGTCCATACGGCATGGTGCTGGTAACAGGGCCTACA
GGCTCGGGTAAAACCGTATCGCTTTACACCTGCTTAAACATTTTGAATACCGATGACGTAAACATCTCCACCGCGGAAGA
CCCTGCGGAGATTAACTTACCAGGCATCAATCAAGTAAACGTAAACGACAAACAAGGCTTAACTTTTGAAGCTGCGCTAA
AAGCATTCTTGCGTCAAGACCCAGACATCATCATGATTGGTGAGATTCGTGACTTGGGTACAGCCGATATTGCGATTAAA
GCCGCACAAACAGGTCACATGGTGTTCTCAACCTTGCACACCAACAATGCCCCAGCCACCTTGTCGCGTATGTTGAACAT
GGGTGTTGCGCCGTTTAATATTGCATCGGCAGTGAACTTGATTATGGCGCAGCGTTTGGCACGTCGTTTGTGCAGCGCGT
GTAAAGCCCCAATGGAACGTCCGCCTGAAAATGTATTGCGCGATGCAGGTTTTACTGACGAAGATTTGGCAAAAGATTGG
ACAATGTATCGTGCCGTAGGTTGTGATGCGTGTAAAGGCAAAGGCTACAAAGGTCGTGCAGGCTTGTACGAAGTGATGCC
GATGACGGAAAAAATGAAAGCCGTTATCATGAAAGGCGGTACAGAAGTGGATATTGCTAACATTGCCTATGAAGAAGGTT
TGTGCGATTTGCGCCGTTCTGGCTTGCTCAAAGTGATGCAAGGTGTAACCACTTTGGAAGAAGTGATTGCGACAACTAAC
GAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(181-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5S8J8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.271

100

0.683
  pilB Acinetobacter baumannii D1279779

52.91

100

0.535
  pilB Acinetobacter baylyi ADP1

51.865

100

0.52
  pilB Legionella pneumophila strain ERS1305867

47.695

100

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.455
  pilB Vibrio campbellii strain DS40M4

45.421

95.365

0.433
  pilB Vibrio cholerae strain A1552

46.318

91.979

0.426
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.808

92.692

0.369