Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LVJ81_RS01615 Genome accession   NZ_CP091512
Coordinates   350250..351941 (+) Length   563 a.a.
NCBI ID   WP_019957099.1    Uniprot ID   -
Organism   Vitreoscilla stercoraria strain SAG 1488-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 345250..356941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ81_RS01600 (LVJ81_01600) secA 345570..348248 (+) 2679 WP_019957096.1 preprotein translocase subunit SecA -
  LVJ81_RS01605 (LVJ81_01605) - 348297..348617 (-) 321 WP_019957097.1 helix-hairpin-helix domain-containing protein -
  LVJ81_RS01610 (LVJ81_01610) - 349075..350115 (+) 1041 WP_019957098.1 porin -
  LVJ81_RS01615 (LVJ81_01615) pilF 350250..351941 (+) 1692 WP_019957099.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ81_RS01620 (LVJ81_01620) pilG 351974..353203 (+) 1230 WP_019957100.1 type II secretion system F family protein Machinery gene
  LVJ81_RS01625 (LVJ81_01625) pilD 353206..354090 (+) 885 WP_019957101.1 A24 family peptidase Machinery gene
  LVJ81_RS01630 (LVJ81_01630) coaE 354087..354689 (+) 603 WP_019957102.1 dephospho-CoA kinase -
  LVJ81_RS01635 (LVJ81_01635) zapD 354698..355486 (+) 789 WP_019957103.1 cell division protein ZapD -
  LVJ81_RS01640 (LVJ81_01640) yacG 355474..355662 (+) 189 WP_019957104.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61512.95 Da        Isoelectric Point: 5.0217

>NTDB_id=651901 LVJ81_RS01615 WP_019957099.1 350250..351941(+) (pilF) [Vitreoscilla stercoraria strain SAG 1488-6]
MDSGLLRILAQQKIITDAQISAFESKAQADGRPIVAIVLQEKIITSQQLAHLLAHLFGCPLFDLSQFDQEKVDFSVITTE
QSAKFLTIPLLQRGRRLTVAVADPSVLQALQKATFSAGVTIDMVVVPADQLLGLIERLGASSSTNILDDFDTDLLGGGTI
GDEKEEEQVEDGPIAKFVHKVLLDALKAGASDIHFEFYEKVARVRLRVDGQLRELINPPIQIREQIASRIKVMSSLDISE
RRIPQDGRIKLKVSDTRAIDFRVSTLPTLFGEKIVMRILDSTAASLNVDQLGFEDFQKEILLDAINRPYGMVLVTGPTGS
GKTVSLYTCLNILNTPDVNISTAEDPAEINLPGINQVNVNDKQGLTFSAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLLRRLCGECKAPVESPPDDVLKKMGFTDEMLTRMKTE
GLQFHGPVGCDRCRGGGYKGRLGVYELMPISSEIQRAIMKNATAVDIADIAYQEGMVDLRRAGLLKVMNGLTSLEEVVAG
TND

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=651901 LVJ81_RS01615 WP_019957099.1 350250..351941(+) (pilF) [Vitreoscilla stercoraria strain SAG 1488-6]
ATGGATAGCGGTTTATTACGTATATTGGCTCAACAAAAAATCATTACTGATGCGCAGATATCGGCTTTTGAAAGCAAAGC
TCAAGCTGATGGTAGACCTATTGTAGCGATTGTATTACAAGAAAAAATCATTACATCTCAACAATTGGCTCATTTGTTAG
CGCATTTATTTGGTTGCCCTTTGTTTGATTTAAGCCAGTTTGATCAAGAGAAAGTAGATTTCTCTGTGATTACTACTGAA
CAAAGTGCCAAGTTCTTAACTATACCTCTATTACAACGAGGACGTCGCTTGACCGTAGCAGTTGCAGACCCATCGGTATT
GCAAGCTTTGCAAAAAGCAACGTTCTCTGCTGGGGTAACCATTGATATGGTTGTGGTTCCAGCAGATCAATTATTAGGTT
TAATTGAACGTTTAGGTGCCAGCTCTTCTACCAATATTTTAGATGATTTTGATACTGATTTATTGGGTGGCGGAACGATT
GGGGATGAAAAAGAAGAAGAGCAAGTTGAAGATGGTCCGATTGCCAAATTTGTACATAAAGTGTTATTGGATGCATTGAA
AGCGGGTGCATCGGATATCCATTTCGAGTTTTATGAAAAAGTGGCACGTGTGCGCTTGCGTGTTGACGGACAATTGCGCG
AGTTGATTAATCCACCGATTCAAATCAGAGAGCAAATTGCTTCGCGTATCAAAGTTATGTCTTCTTTGGATATTTCAGAA
AGACGCATTCCTCAAGACGGCCGTATTAAATTGAAAGTCAGCGATACTCGTGCCATCGACTTTCGTGTGAGTACTTTGCC
AACGTTGTTTGGCGAAAAAATTGTAATGCGTATTTTGGACTCAACAGCGGCATCGCTGAATGTTGATCAATTGGGTTTTG
AGGATTTCCAGAAAGAAATCTTGTTAGATGCGATTAACCGTCCATATGGCATGGTGCTAGTAACAGGCCCAACAGGTTCG
GGTAAAACGGTTTCTTTGTATACTTGTTTGAACATTTTGAATACGCCTGATGTCAATATTTCTACTGCAGAGGATCCAGC
AGAGATTAACTTGCCTGGCATTAATCAGGTGAATGTGAATGACAAACAAGGTTTAACGTTCTCAGCGGCCTTAAAGTCTT
TTTTACGCCAAGATCCCGATATTATTATGGTGGGTGAAATCCGTGATTTGGAAACTGCCGATATTGCCATTAAGGCCGCC
CAAACTGGCCATATGGTATTTTCCACACTGCATACCAATAATGCGCCAGCAACTTTATCGCGCATGTTAAATATGGGTGT
GGCACCTTTTAATATTGCCAGTTCAGTGAATTTGATTATGGCGCAACGTCTGTTGCGTCGTTTGTGTGGCGAATGTAAAG
CACCTGTTGAATCTCCTCCAGATGATGTTTTGAAAAAAATGGGCTTCACTGATGAAATGTTGACCAGAATGAAAACTGAA
GGCTTGCAGTTTCATGGTCCTGTTGGGTGTGATCGTTGTCGTGGCGGTGGCTATAAAGGTCGTTTGGGTGTGTATGAATT
GATGCCGATTAGTAGTGAAATTCAGCGTGCTATTATGAAAAATGCGACTGCAGTTGATATTGCTGACATTGCTTATCAAG
AAGGTATGGTAGATTTACGCCGTGCAGGTTTGTTAAAAGTGATGAATGGATTGACTTCGTTGGAAGAAGTGGTTGCAGGC
ACTAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(180-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

63.009

100

0.632
  pilB Acinetobacter baumannii D1279779

51.699

99.29

0.513
  pilB Acinetobacter baylyi ADP1

50.532

100

0.506
  pilB Legionella pneumophila strain ERS1305867

48.135

100

0.481
  pilB Vibrio cholerae strain A1552

46.726

100

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

45.487

100

0.456
  pilB Vibrio campbellii strain DS40M4

45.31

100

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

39.099

98.579

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.603

96.625

0.373
  pilF Thermus thermophilus HB27

37.319

98.046

0.366