Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L3V47_RS18920 Genome accession   NZ_CP091480
Coordinates   4128878..4130584 (+) Length   568 a.a.
NCBI ID   WP_058394588.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain ASscau0313     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4123878..4135584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3V47_RS18895 (L3V47_18860) pdhR 4125010..4125774 (-) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  L3V47_RS18900 (L3V47_18865) ampD 4126051..4126623 (-) 573 WP_277735130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L3V47_RS18905 (L3V47_18870) - 4126772..4127242 (+) 471 WP_011898292.1 retropepsin-like aspartic protease family protein -
  L3V47_RS18910 (L3V47_18875) nadC 4127247..4128104 (+) 858 WP_277735131.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L3V47_RS18915 (L3V47_18880) tapA 4128440..4128874 (+) 435 WP_277735133.1 type IVa pilus major pilin TapA -
  L3V47_RS18920 (L3V47_18885) pilB 4128878..4130584 (+) 1707 WP_058394588.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  L3V47_RS18925 (L3V47_18890) pilC 4130717..4131958 (+) 1242 WP_021140339.1 type II secretion system F family protein Machinery gene
  L3V47_RS18930 (L3V47_18895) pilD 4132022..4132897 (+) 876 WP_277735136.1 prepilin peptidase Machinery gene
  L3V47_RS18935 (L3V47_18900) coaE 4132917..4133531 (+) 615 WP_265433253.1 dephospho-CoA kinase -
  L3V47_RS18940 (L3V47_18905) zapD 4133569..4134291 (+) 723 WP_005314166.1 cell division protein ZapD -
  L3V47_RS18945 (L3V47_18910) yacG 4134301..4134495 (+) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  L3V47_RS18950 (L3V47_18915) mutT 4134572..4134979 (-) 408 WP_005314168.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62546.97 Da        Isoelectric Point: 5.4384

>NTDB_id=651636 L3V47_RS18920 WP_058394588.1 4128878..4130584(+) (pilB) [Aeromonas salmonicida strain ASscau0313]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=651636 L3V47_RS18920 WP_058394588.1 4128878..4130584(+) (pilB) [Aeromonas salmonicida strain ASscau0313]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGCTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTTAATACCGCCGACGACGATGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGTTTTCGGATCGATGGCATACTGCACGAGATAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCCCGACTCGATATCGCCGAGCGACGGCTGCCCCAGGATGGTCGTATCAAGTTAAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CGGCGCGGCTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCACAGTATCTGCGGGCCCTCTCCAAGCCCCAG
GGCATGATTCTGGTGACAGGTCCGACCGGCTCTGGCAAGACGGTCTCTCTCTATACCGGCCTCAATATTCTCAACACCAC
AGAGGTCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGTTGACCTTCGCCAGCGCCCTGCGATCCTTTCTTCGCCAGGATCCCGATGTGGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCTGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCTTCGGTAACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCGCCGGAGGTAGTGCCCGAGGCTGAACTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAACCGGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAATATTGCAAAATTGATCATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CCATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Acinetobacter baumannii D1279779

55.556

99.824

0.555
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Vibrio cholerae strain A1552

54.513

99.472

0.542
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375