Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L4F93_RS06955 Genome accession   NZ_CP091456
Coordinates   1454425..1455849 (+) Length   474 a.a.
NCBI ID   WP_250349623.1    Uniprot ID   -
Organism   Avibacterium sp. 20-132     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1449425..1460849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L4F93_RS06925 (L4F93_06925) lgt 1449714..1450514 (-) 801 WP_250349617.1 prolipoprotein diacylglyceryl transferase -
  L4F93_RS06930 (L4F93_06930) - 1450525..1451316 (-) 792 WP_250349618.1 sulfite exporter TauE/SafE family protein -
  L4F93_RS06935 (L4F93_06935) rppH 1451316..1451933 (-) 618 WP_250349619.1 RNA pyrophosphohydrolase -
  L4F93_RS06940 (L4F93_06940) - 1452608..1453207 (+) 600 WP_250349620.1 cation diffusion facilitator family transporter -
  L4F93_RS06945 (L4F93_06945) ampD 1453273..1453827 (-) 555 WP_250349621.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L4F93_RS06950 (L4F93_06950) pilA 1453978..1454412 (+) 435 WP_250349622.1 prepilin peptidase-dependent pilin Machinery gene
  L4F93_RS06955 (L4F93_06955) pilB 1454425..1455849 (+) 1425 WP_250349623.1 GspE/PulE family protein Machinery gene
  L4F93_RS06960 (L4F93_06960) - 1455899..1460476 (-) 4578 WP_250349624.1 exo-alpha-sialidase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54099.99 Da        Isoelectric Point: 6.0320

>NTDB_id=651444 L4F93_RS06955 WP_250349623.1 1454425..1455849(+) (pilB) [Avibacterium sp. 20-132]
MMSVEEQIREEEILVISNNGEEFRLSPKLWQKNKQNKEILLRYFACPIQEDLQTLWLAIDDLKNISACEVFSFLTGKLIE
PILLDNAQLKAILQKLTPQVQNHNVEDVSSFYVVNNEEMEQNQAELDEPVIRQLDHLFELALQHNASDIHLEPQVMALQV
RFRIDGVLQCQQALPKTLAKRLISRLKLLAKLDISETRLPQDGRFHFKTTFSDVLDFRLSTLPTHQGEKAVLRLQKNKPV
NFDFSELGMTSVQQQTLQQALSQPQGLILVTGPTGSGKSITLYTALEWLNQSEKHILTAEDPIEIELPGIIQTQINPQIG
LDFNRLLRTFLRQDPDIIMLGEIRDAESASIALRAAQTGHLVLSTLHTNDAHSAISRLLQLGIQQHEIDNSLLLVIAQRL
VRKKCHHLTKDCDCYQGYKGRSGIYQFLQPHFYNGERSYQTDYASLRESGLEKVKEGITDLAEVERVLGKRMDK

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=651444 L4F93_RS06955 WP_250349623.1 1454425..1455849(+) (pilB) [Avibacterium sp. 20-132]
ATGATGAGTGTTGAAGAACAAATTCGAGAAGAAGAGATTTTAGTTATATCTAATAATGGGGAAGAATTTAGATTATCACC
AAAGTTATGGCAAAAGAATAAACAAAATAAGGAAATTTTATTACGTTATTTTGCTTGCCCTATTCAAGAAGATTTACAAA
CGCTATGGCTTGCTATTGATGATTTAAAAAATATCTCGGCATGTGAAGTATTTTCCTTTTTAACTGGAAAACTCATTGAG
CCTATACTGTTAGACAATGCTCAGCTAAAAGCAATATTGCAAAAACTTACGCCACAAGTACAGAATCATAATGTAGAAGA
TGTATCTAGTTTTTATGTCGTAAATAATGAAGAAATGGAGCAAAATCAAGCAGAGCTAGATGAGCCGGTCATCAGGCAAC
TAGATCATCTTTTTGAACTTGCATTACAACACAATGCTTCTGATATTCATCTTGAACCTCAAGTAATGGCGTTACAAGTT
CGTTTTAGAATTGATGGGGTTTTGCAATGTCAACAGGCGTTACCTAAAACCTTAGCAAAGCGGTTAATTTCACGTTTGAA
GCTACTGGCGAAATTAGATATTAGTGAAACGCGTCTACCACAAGATGGACGTTTTCATTTTAAAACTACTTTTTCTGATG
TATTAGATTTTCGTTTATCTACTTTACCAACGCATCAAGGAGAAAAAGCTGTACTCCGATTACAAAAAAATAAGCCAGTA
AATTTTGACTTCTCTGAATTAGGTATGACTAGTGTACAGCAGCAAACGTTACAGCAAGCACTTTCTCAACCCCAAGGTTT
GATTTTGGTTACAGGCCCAACAGGGAGCGGAAAAAGTATCACCCTTTATACCGCACTTGAGTGGTTAAATCAGTCAGAAA
AACATATTCTCACCGCAGAAGATCCCATTGAAATCGAGCTACCGGGCATTATCCAAACACAAATTAATCCCCAAATTGGG
CTTGACTTTAATCGCCTATTACGCACTTTTTTAAGGCAAGACCCCGATATCATTATGCTTGGTGAAATTCGTGATGCAGA
AAGTGCTTCAATTGCCTTGCGTGCCGCACAAACAGGGCATTTAGTACTTTCTACATTGCATACGAATGATGCACATTCAG
CGATCTCTCGTTTGTTGCAACTTGGTATCCAACAGCACGAAATTGACAATAGTCTATTGCTTGTTATTGCACAGCGATTG
GTACGAAAGAAATGTCATCATTTGACTAAAGATTGCGATTGCTATCAAGGCTATAAAGGGCGCAGTGGCATTTACCAATT
TTTACAACCGCACTTTTATAATGGGGAACGTTCCTATCAAACGGATTATGCTTCCCTACGAGAAAGTGGATTGGAGAAAG
TAAAAGAAGGAATAACAGATTTAGCTGAGGTAGAACGAGTATTAGGGAAACGGATGGATAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(23-98)

Type II/IV secretion system protein (T2SSE)

(139-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

69.114

97.679

0.675
  pilB Haemophilus influenzae 86-028NP

68.913

97.046

0.669
  pilB Glaesserella parasuis strain SC1401

60.31

95.148

0.574
  pilB Vibrio parahaemolyticus RIMD 2210633

41.164

100

0.418
  pilB Vibrio cholerae strain A1552

40.664

100

0.414
  pilB Vibrio campbellii strain DS40M4

39.709

100

0.403
  pilB Legionella pneumophila strain ERS1305867

38.65

100

0.399
  pilB Acinetobacter baylyi ADP1

37.177

100

0.395
  pilB Acinetobacter baumannii D1279779

38.066

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.641

99.367

0.384
  pilF Thermus thermophilus HB27

37.924

99.578

0.378
  pilF Neisseria gonorrhoeae MS11

36.364

100

0.371