Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L4F93_RS06950 Genome accession   NZ_CP091456
Coordinates   1453978..1454412 (+) Length   144 a.a.
NCBI ID   WP_250349622.1    Uniprot ID   -
Organism   Avibacterium sp. 20-132     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1448978..1459412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L4F93_RS06925 (L4F93_06925) lgt 1449714..1450514 (-) 801 WP_250349617.1 prolipoprotein diacylglyceryl transferase -
  L4F93_RS06930 (L4F93_06930) - 1450525..1451316 (-) 792 WP_250349618.1 sulfite exporter TauE/SafE family protein -
  L4F93_RS06935 (L4F93_06935) rppH 1451316..1451933 (-) 618 WP_250349619.1 RNA pyrophosphohydrolase -
  L4F93_RS06940 (L4F93_06940) - 1452608..1453207 (+) 600 WP_250349620.1 cation diffusion facilitator family transporter -
  L4F93_RS06945 (L4F93_06945) ampD 1453273..1453827 (-) 555 WP_250349621.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L4F93_RS06950 (L4F93_06950) pilA 1453978..1454412 (+) 435 WP_250349622.1 prepilin peptidase-dependent pilin Machinery gene
  L4F93_RS06955 (L4F93_06955) pilB 1454425..1455849 (+) 1425 WP_250349623.1 GspE/PulE family protein Machinery gene

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15387.70 Da        Isoelectric Point: 6.8992

>NTDB_id=651443 L4F93_RS06950 WP_250349622.1 1453978..1454412(+) (pilA) [Avibacterium sp. 20-132]
MQRSSVIRINKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAISELLQAAAPYRADVELCIYNTGEKQHCNAGTNGI
QNKLENKGKVKSIDVKSGVITVTGQGALEEISYTLTVDGDVSSGISWTVDCGSSADLFPAGFCQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=651443 L4F93_RS06950 WP_250349622.1 1453978..1454412(+) (pilA) [Avibacterium sp. 20-132]
ATGCAAAGATCCAGCGTAATACGTATTAATAAGAAAGGATTCACCCTTATTGAGCTAATGATTGTTATTGCTATTATTGC
AATTTTGGCAACTATTGCAATTCCATCGTATCAAAATTATACCAAAAAAGCGGCAATTTCCGAATTACTACAAGCTGCCG
CCCCCTACAGAGCAGATGTGGAACTATGTATTTATAATACAGGTGAAAAACAACATTGTAATGCTGGTACAAATGGTATT
CAGAATAAATTAGAAAATAAAGGAAAGGTTAAATCAATTGATGTGAAGAGTGGCGTTATAACAGTAACGGGGCAAGGAGC
TTTAGAAGAAATCAGTTATACACTTACTGTTGATGGTGATGTAAGTTCGGGAATTTCTTGGACTGTTGACTGTGGTTCAT
CAGCAGACTTATTTCCAGCAGGTTTCTGTCAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-140)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

59.441

99.306

0.59
  pilA Haemophilus influenzae 86-028NP

62.963

93.75

0.59
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.857

97.222

0.368
  pilA Vibrio cholerae strain A1552

37.857

97.222

0.368
  pilA Vibrio cholerae C6706

37.857

97.222

0.368