Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LZ558_RS03360 Genome accession   NZ_CP091424
Coordinates   750134..751849 (-) Length   571 a.a.
NCBI ID   WP_268119416.1    Uniprot ID   -
Organism   Methylobacter sp. YRD-M1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 745134..756849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ558_RS03330 (LZ558_03305) - 745438..746367 (+) 930 WP_268119410.1 Nudix family hydrolase -
  LZ558_RS03335 (LZ558_03310) yacG 746369..746569 (-) 201 WP_268119411.1 DNA gyrase inhibitor YacG -
  LZ558_RS03340 (LZ558_03315) zapD 746570..747340 (-) 771 WP_268119412.1 cell division protein ZapD -
  LZ558_RS03345 (LZ558_03320) coaE 747354..747950 (-) 597 WP_268119413.1 dephospho-CoA kinase -
  LZ558_RS03350 (LZ558_03325) pilD 747955..748830 (-) 876 WP_268120765.1 A24 family peptidase Machinery gene
  LZ558_RS03355 (LZ558_03330) pilC 748911..750131 (-) 1221 WP_268119414.1 type II secretion system F family protein Machinery gene
  LZ558_RS03360 (LZ558_03335) pilB 750134..751849 (-) 1716 WP_268119416.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LZ558_RS03365 (LZ558_03340) - 751981..754788 (-) 2808 WP_268119417.1 valine--tRNA ligase -
  LZ558_RS03370 (LZ558_03345) - 754864..755310 (-) 447 WP_268119418.1 DNA polymerase III subunit chi -
  LZ558_RS03375 (LZ558_03350) - 755317..756807 (-) 1491 WP_268119419.1 leucyl aminopeptidase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63133.86 Da        Isoelectric Point: 6.1213

>NTDB_id=651087 LZ558_RS03360 WP_268119416.1 750134..751849(-) (pilB) [Methylobacter sp. YRD-M1]
MATVLTDLQFNGLAKCLIQGGLLSESDAQTHIQEAQKRKIPLISYLVANNLVDSRAVATRASTEFGIPFFDLDAIDCRSL
PVKVISEKLIRQHHVLPLFMRGKTLFIAVSDPTNFQALDEIKFHSLLKPETILVEENKLAKAIETSLEAAADTSMKDLLL
DEDLEKLNITGGDENASEDINSEIDDAPIVRFVNKILLDSIKKGASDIHLEPYEKNFRIRFRSDGILHEIASPPVSAANR
IISRIKVMARMDIAERRVPQDGRIKMSLSKHRAIDFRVNTCPTLFGEKVVLRILDPTSAQIGIERLGFEPEQEKLFLEAI
HKPYGMVLVTGPTGSGKTVTLYTGLNILNSIERNISTAEDPVEITVEGINQVNMNPKAGLTFASALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPPFNIVSAVNLIMAQRLARRLCEHCKKPADYPAKALVS
AGFKEEELDNLQLFSPVGCEHCTNGYKGRVGIYQVMTLSEKMRSLILEGGNAMQLAEQAKSEGINDLRASGLNKVRVGIT
TLEEIDRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=651087 LZ558_RS03360 WP_268119416.1 750134..751849(-) (pilB) [Methylobacter sp. YRD-M1]
ATGGCTACCGTATTAACCGATCTACAATTCAATGGTCTCGCAAAATGCTTGATTCAAGGCGGCCTGCTGTCTGAAAGTGA
CGCCCAAACCCACATTCAGGAAGCGCAAAAAAGGAAAATCCCGCTCATTTCCTATCTGGTCGCCAATAATCTGGTTGACA
GCAGAGCCGTAGCAACAAGAGCCTCCACTGAATTCGGCATACCTTTTTTTGATCTGGATGCGATAGACTGCCGCAGCCTG
CCTGTCAAAGTGATCAGCGAGAAACTGATTCGTCAGCATCATGTGCTGCCGCTGTTTATGCGTGGCAAAACGCTGTTTAT
AGCTGTCTCCGATCCGACCAATTTCCAGGCACTCGATGAAATCAAGTTTCATAGCCTGCTTAAACCCGAAACCATACTGG
TCGAGGAAAACAAACTCGCCAAGGCAATTGAAACCTCTTTAGAAGCAGCGGCCGATACGTCCATGAAGGATCTTCTGCTT
GACGAAGATCTGGAGAAACTGAATATTACGGGCGGTGATGAAAATGCGAGCGAGGATATCAATTCTGAAATTGACGATGC
CCCCATTGTGCGTTTCGTCAATAAAATCCTGCTGGATTCGATAAAAAAAGGCGCTTCCGACATTCATCTGGAGCCCTATG
AAAAAAACTTCCGTATCCGATTCAGGTCTGACGGCATCCTGCACGAAATCGCCAGTCCCCCGGTCAGTGCCGCGAACCGC
ATTATCTCGCGCATCAAAGTCATGGCCAGGATGGATATTGCCGAGCGCCGGGTTCCGCAGGACGGCCGTATCAAGATGTC
CTTATCGAAGCATCGCGCGATTGACTTTCGCGTCAACACCTGTCCGACACTGTTCGGCGAAAAAGTCGTTCTGCGTATTC
TCGATCCCACCAGCGCTCAAATCGGCATCGAAAGGCTAGGCTTTGAACCGGAACAGGAAAAGCTGTTTCTTGAGGCTATC
CATAAACCTTACGGCATGGTCCTCGTTACCGGGCCAACCGGTAGCGGCAAAACAGTCACGCTCTATACAGGCCTGAACAT
CCTCAACAGCATCGAGCGCAATATTTCCACGGCCGAGGATCCTGTTGAAATCACGGTAGAAGGCATCAATCAGGTCAATA
TGAATCCCAAAGCTGGCCTGACGTTCGCCTCGGCCCTGCGCGCCTTTTTGCGCCAGGACCCTGATATCATCATGGTCGGC
GAAATTCGCGACCTGGAAACTGCAGAAATCGCCGTCAAAGCGGCACAGACAGGCCACTTGGTATTATCGACCCTGCATAC
CAATGACGCCCCGCAAACATTGAACCGTCTCATGCAGATGGGCATTCCGCCTTTCAATATCGTCTCGGCCGTCAACCTGA
TCATGGCTCAGCGCCTGGCTCGGCGCCTGTGCGAGCATTGCAAGAAACCGGCCGACTACCCGGCCAAAGCCCTGGTCTCT
GCAGGCTTTAAAGAAGAAGAGCTGGATAACCTGCAACTATTCAGTCCTGTAGGCTGTGAGCATTGTACGAACGGCTATAA
AGGCCGGGTCGGCATCTATCAAGTCATGACATTGAGTGAGAAAATGCGTTCGCTCATTCTTGAAGGCGGCAATGCCATGC
AACTCGCAGAGCAGGCAAAGTCCGAAGGCATTAATGATTTGCGGGCATCGGGCTTGAACAAGGTTCGTGTGGGTATTACG
ACTCTCGAAGAAATTGACCGCGTAACGAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.594

100

0.557
  pilB Acinetobacter baumannii D1279779

53.403

100

0.536
  pilB Acinetobacter baylyi ADP1

52.88

100

0.531
  pilF Neisseria gonorrhoeae MS11

47.679

98.074

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

47.237

98.249

0.464
  pilB Vibrio cholerae strain A1552

46.643

99.124

0.462
  pilB Vibrio campbellii strain DS40M4

46.643

99.124

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

40.761

96.673

0.394
  pilF Thermus thermophilus HB27

39.964

97.723

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.584

98.949

0.382