Detailed information NTG0650108

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L2Z22_RS02420
• Genome accession: NZ_CP091353
• Coordinates: 499804..500922 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain AB189-VUB
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 494804..505922

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Z22_RS02390 (L2Z22_02390)	-	495633..496481 (+)	849	WP_000336548.1	class II glutamine amidotransferase	-
L2Z22_RS02395 (L2Z22_02395)	-	496734..497189 (+)	456	WP_000782976.1	bacteriohemerythrin	-
L2Z22_RS02400 (L2Z22_02400)	-	497342..498409 (+)	1068	WP_002019572.1	hypothetical protein	-
L2Z22_RS02405 (L2Z22_02405)	-	498409..498732 (+)	324	WP_000442587.1	RnfH family protein	-
L2Z22_RS02410 (L2Z22_02410)	bamE	498787..499185 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
L2Z22_RS02415 (L2Z22_02415)	fur	499297..499734 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
L2Z22_RS02420 (L2Z22_02420)	pilU	499804..500922 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
L2Z22_RS02425 (L2Z22_02425)	pilT	500950..501987 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
L2Z22_RS02430 (L2Z22_02430)	-	502114..502806 (+)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=650108 L2Z22_RS02420 WP_000347039.1 499804..500922(-) (pilU) [Acinetobacter baumannii strain AB189-VUB]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=650108 L2Z22_RS02420 WP_000347039.1 499804..500922(-) (pilU) [Acinetobacter baumannii strain AB189-VUB]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395