Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L2Y54_RS21045 Genome accession   NZ_CP091244
Coordinates   4255858..4257579 (+) Length   573 a.a.
NCBI ID   WP_236498846.1    Uniprot ID   -
Organism   Thiothrix winogradskyi strain CT3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4250858..4262579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y54_RS21025 (L2Y54_21025) - 4251319..4251696 (+) 378 WP_236498840.1 MarR family transcriptional regulator -
  L2Y54_RS21030 (L2Y54_21030) - 4251764..4252594 (+) 831 WP_236498842.1 hypothetical protein -
  L2Y54_RS21035 (L2Y54_21035) - 4252632..4254170 (+) 1539 WP_236498843.1 ATP-binding protein -
  L2Y54_RS21040 (L2Y54_21040) - 4254220..4255776 (+) 1557 WP_236498844.1 ATP-binding protein -
  L2Y54_RS21045 (L2Y54_21045) pilB 4255858..4257579 (+) 1722 WP_236498846.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L2Y54_RS21050 (L2Y54_21050) pilC 4257672..4258922 (+) 1251 WP_236498847.1 type II secretion system F family protein Machinery gene
  L2Y54_RS21055 (L2Y54_21055) pilD 4258931..4259806 (+) 876 WP_236498848.1 prepilin peptidase Machinery gene
  L2Y54_RS21060 (L2Y54_21060) - 4259817..4260221 (-) 405 WP_236498850.1 hypothetical protein -
  L2Y54_RS21065 (L2Y54_21065) ubiE 4260303..4261055 (+) 753 WP_236498851.1 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  L2Y54_RS21070 (L2Y54_21070) opgC 4261073..4262248 (-) 1176 WP_236498852.1 OpgC domain-containing protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62833.65 Da        Isoelectric Point: 7.1539

>NTDB_id=648801 L2Y54_RS21045 WP_236498846.1 4255858..4257579(+) (pilB) [Thiothrix winogradskyi strain CT3]
MTPLTSSIPLPGLARQLVVQDLLDKESATQAVQKARAEKVSLVDWMVRSRIVSTKDVAYAASLEYGLSMVDLDQISIPPL
IAALLSPEQMRKLNLIPLYQRGKNLMVGLADPTYLTNLDDVKFATGLIPEPVFVEFDKLQRYISSDDGISGHAAPTHGGK
ASPSAAPVAAAKEIALDVRDLKTKDDEDPAVIEFVDKLLAHGIKSKASDIHIEIFEKNMRIRYRIDGILQVVANPPNDSA
QQLISRIKVLARMNISERRVPQDGRIRFTVNDKQKIDFRVNTLPTLFGEKVVMRILDSSNAALGVEKLGFEPKQQHDFMK
ALDKPDGMILVTGPTGSGKTVTLYTGLGILNTPEKNISTAEDPAEINMPGVNQVNVNAKVGLTFAEALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIASSIHLIIAQRLARRLCEKCKQPSSVPREVL
LQMGFQPDELSSLKLYEPVGCSECSRGYKGRVGIYQVMPISAEMGRMIMNGSNSLEIAEQALKEGIADLRRSALNKVRQG
ITSLAELERVTSD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=648801 L2Y54_RS21045 WP_236498846.1 4255858..4257579(+) (pilB) [Thiothrix winogradskyi strain CT3]
ATGACACCGCTGACTTCTTCCATCCCTTTACCGGGGTTAGCCAGACAATTAGTTGTGCAAGACTTGCTCGACAAAGAGTC
GGCGACGCAAGCCGTGCAGAAAGCGCGTGCGGAAAAAGTCTCACTGGTGGACTGGATGGTTCGCTCCCGGATCGTATCCA
CCAAAGATGTCGCCTATGCCGCCAGCCTCGAATACGGCTTAAGCATGGTCGACCTCGATCAAATCAGCATTCCACCGCTG
ATTGCGGCACTGCTCAGCCCAGAGCAGATGCGCAAACTCAACCTGATTCCCCTGTATCAACGCGGCAAAAATCTCATGGT
GGGCTTGGCTGACCCCACTTACTTGACCAATCTGGATGATGTCAAATTTGCCACCGGCTTAATCCCCGAACCCGTGTTTG
TCGAGTTCGACAAACTGCAACGCTATATCAGCAGCGACGATGGTATAAGCGGACACGCTGCCCCAACCCACGGTGGTAAA
GCTTCTCCCTCTGCCGCGCCAGTAGCCGCTGCCAAAGAAATTGCCTTAGATGTCCGCGACCTCAAAACCAAGGACGACGA
AGACCCCGCCGTCATTGAATTCGTCGACAAGCTGTTAGCCCACGGCATTAAATCCAAAGCGTCGGATATTCACATTGAGA
TTTTTGAAAAAAATATGCGGATCCGCTACCGGATAGACGGCATTTTGCAAGTGGTTGCCAACCCGCCCAACGATTCCGCC
CAACAATTGATTTCGCGCATCAAAGTCTTGGCACGCATGAATATTTCCGAACGCCGCGTCCCTCAAGACGGGCGTATCCG
TTTTACGGTCAATGACAAACAAAAAATCGACTTCCGGGTGAATACCCTGCCCACCCTGTTCGGTGAGAAAGTGGTTATGC
GGATACTCGACTCTTCCAACGCCGCATTGGGGGTAGAAAAGCTCGGTTTTGAACCCAAGCAACAACACGATTTCATGAAA
GCGCTGGACAAACCCGATGGCATGATTCTGGTCACAGGCCCCACCGGTAGCGGTAAAACCGTCACGCTGTACACCGGTTT
AGGCATCCTCAACACCCCAGAAAAAAACATTTCCACCGCCGAAGACCCCGCTGAAATCAATATGCCCGGCGTGAACCAAG
TCAACGTCAATGCCAAAGTCGGGCTGACCTTTGCCGAAGCCTTGCGCTCTTTCTTGCGTCAAGACCCGGACATCATCATG
GTGGGGGAAATCCGCGACCTTGAAACCGCTGAAATTGCCATCAAAGCGGCACAAACGGGACACTTAGTGTTATCCACCCT
GCACACCAATAGCGCCCCGGAAACCCTGACCCGTTTAATGAACATGGGAGTACCGACATTTAATATCGCCTCTTCCATTC
ACTTAATTATTGCCCAACGTTTGGCGCGACGTTTATGTGAAAAGTGCAAGCAACCGTCCAGTGTTCCCCGGGAAGTGCTG
CTGCAAATGGGCTTCCAACCTGACGAACTCAGTTCACTCAAACTCTATGAACCGGTCGGATGTTCGGAATGTTCACGCGG
CTATAAAGGTCGAGTAGGTATTTACCAAGTCATGCCGATTTCTGCTGAAATGGGGCGCATGATCATGAATGGCAGCAACT
CGCTGGAAATCGCCGAACAAGCCCTCAAAGAAGGCATTGCGGATTTGCGCCGTTCTGCGCTCAACAAAGTGCGGCAGGGC
ATCACCTCATTGGCAGAGCTGGAACGTGTCACCTCAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

49.746

100

0.513
  pilB Acinetobacter baumannii D1279779

50.342

100

0.513
  pilB Legionella pneumophila strain ERS1305867

50

100

0.504
  pilF Neisseria gonorrhoeae MS11

48.41

98.778

0.478
  pilB Vibrio cholerae strain A1552

47.292

96.684

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

46.961

94.764

0.445
  pilB Vibrio campbellii strain DS40M4

46.606

95.113

0.443
  pilF Thermus thermophilus HB27

38.421

99.476

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.154

94.939

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.41

97.033

0.363