Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L2O48_RS08430 Genome accession   NZ_CP091185
Coordinates   1758132..1759820 (-) Length   562 a.a.
NCBI ID   WP_029189846.1    Uniprot ID   A0AAW4AT81
Organism   Vibrio anguillarum strain J382     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1753132..1764820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2O48_RS08400 (L2O48_08400) rplS 1753850..1754203 (+) 354 WP_010319525.1 50S ribosomal protein L19 -
  L2O48_RS08405 (L2O48_08405) yacG 1754284..1754481 (-) 198 WP_010319526.1 DNA gyrase inhibitor YacG -
  L2O48_RS08410 (L2O48_08410) zapD 1754571..1755311 (-) 741 WP_013857689.1 cell division protein ZapD -
  L2O48_RS08415 (L2O48_08415) coaE 1755344..1755952 (-) 609 WP_013857688.1 dephospho-CoA kinase -
  L2O48_RS08420 (L2O48_08420) pilD 1755943..1756824 (-) 882 WP_013857687.1 A24 family peptidase Machinery gene
  L2O48_RS08425 (L2O48_08425) pilC 1756863..1758089 (-) 1227 WP_013857686.1 type II secretion system F family protein Machinery gene
  L2O48_RS08430 (L2O48_08430) pilB 1758132..1759820 (-) 1689 WP_029189846.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L2O48_RS08435 (L2O48_08435) pilA 1759830..1760255 (-) 426 WP_040122945.1 pilin Machinery gene
  L2O48_RS08440 (L2O48_08440) nadC 1760514..1761401 (-) 888 WP_013857682.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L2O48_RS08445 (L2O48_08445) ampD 1761526..1762077 (+) 552 WP_202599069.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L2O48_RS08450 (L2O48_08450) fldB 1762099..1762617 (-) 519 WP_013857680.1 flavodoxin FldB -
  L2O48_RS08455 (L2O48_08455) xerD 1762770..1763678 (+) 909 WP_013857679.1 site-specific tyrosine recombinase XerD -
  L2O48_RS08460 (L2O48_08460) - 1763692..1764465 (+) 774 WP_013857678.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62241.36 Da        Isoelectric Point: 6.3768

>NTDB_id=648232 L2O48_RS08430 WP_029189846.1 1758132..1759820(-) (pilB) [Vibrio anguillarum strain J382]
MLTNLPAILRQAQILSPTQEHAVIEHIKASGASVPEALLALDFFHPHDLTEQLSTLFGLPDTDLTRYDYPQLCQQLGLRD
LITRYQALPIANNGQTLTIAVSDPTHLQVEDDFRFATGLQVELVLADYKALQAAIRRLYGRTIQGSSSSGKEISQDELAN
LVKLSDDEIQSIEDLSQDDAPVSRFINQILLDAVRKGASDIHFEPYEALYRVRLRCDGILVETQRPSSNLSRRLAARLKI
LSKLDIAERRLPQDGRIKLRLNQDTAIDMRVSSLPTLWGEKIVLRLLDSSAANLDIDKLGYNDQQKQLYLAALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTAEVNISTAEDPVEINLSGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLSNMGIEAFNLASSLSLIIAQRLARRLCRHCKIAVVPSMQLQQHFQLLATE
TIYEANPHGCNECTGGYSGRVGIYEVMAFNPQLAEAIMQKASIHQIEQIAKANGMQTLQESGIEKLKNGVTSFKELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=648232 L2O48_RS08430 WP_029189846.1 1758132..1759820(-) (pilB) [Vibrio anguillarum strain J382]
ATGCTAACCAACCTACCTGCGATTCTGCGTCAGGCTCAGATATTGAGCCCGACGCAAGAACACGCAGTTATTGAACATAT
CAAAGCGTCTGGTGCGAGTGTGCCAGAAGCTTTGCTCGCTTTGGATTTTTTCCATCCTCATGATCTGACTGAGCAGTTAA
GTACCCTTTTTGGCCTACCGGACACCGACTTAACCCGCTACGACTACCCGCAGCTCTGCCAACAGCTTGGTTTGCGTGAT
CTCATCACCCGTTACCAAGCACTGCCGATTGCCAATAATGGCCAAACCCTGACCATCGCGGTTTCCGACCCGACCCATTT
ACAAGTGGAAGATGATTTTCGCTTTGCGACAGGCTTACAAGTCGAACTGGTTTTAGCCGACTACAAAGCGCTGCAAGCAG
CCATCCGGCGCTTGTATGGCCGAACAATTCAAGGATCCTCTTCTTCAGGGAAAGAGATCAGCCAAGATGAACTGGCGAAC
TTAGTTAAGCTGTCTGATGATGAGATTCAATCGATTGAAGACCTCAGCCAAGATGACGCCCCAGTCAGTCGCTTTATTAA
CCAAATCCTGCTTGATGCGGTGCGAAAAGGCGCATCCGATATCCATTTCGAACCGTACGAAGCGCTTTACCGAGTACGAT
TGCGCTGTGATGGTATTTTGGTGGAAACGCAGCGTCCCTCAAGCAACTTAAGTCGCCGGCTTGCCGCGCGGCTTAAAATT
TTATCCAAACTCGATATTGCCGAGCGGCGCTTACCACAAGATGGACGAATTAAACTGCGGTTGAATCAAGACACCGCCAT
CGATATGCGCGTTTCCAGCCTCCCTACTTTATGGGGTGAAAAAATCGTACTGCGCCTACTTGATAGCAGTGCTGCTAACT
TAGATATCGACAAATTGGGTTATAACGACCAGCAAAAACAACTCTACTTAGCTGCGCTGAAAAAACCACAAGGGATGATT
TTAATGACGGGCCCGACGGGTAGCGGAAAAACCGTCTCGCTCTATACGGGGCTACGCATTTTAAATACTGCTGAAGTGAA
CATTTCCACCGCTGAAGATCCGGTAGAAATTAACTTATCCGGTATCAACCAAGTTCAAGTGCAGCCAAAAATTGGCTTTG
GCTTTGCACAAGCATTACGCTCCTTCTTACGGCAAGATCCCGACATTGTGATGGTTGGCGAAATCCGCGATTTAGAAACT
GCCGAAATTGCCGTAAAAGCAGCACAAACCGGGCATTTAGTCCTTTCTACCTTGCATACTAACTCCGCCGCCGAAACGGT
GATTCGCTTAAGTAATATGGGAATTGAAGCGTTTAACTTAGCCTCTTCACTCAGTTTAATTATTGCCCAGCGTTTAGCTC
GTCGCCTTTGTCGGCACTGCAAAATTGCCGTCGTGCCCTCAATGCAGTTGCAGCAACATTTTCAACTGCTAGCGACAGAA
ACCATTTATGAAGCAAACCCGCATGGTTGCAACGAATGCACAGGGGGCTATTCAGGTCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAACCCACAGCTGGCCGAAGCGATTATGCAAAAAGCAAGCATTCATCAAATAGAGCAGATCGCGAAAGCCAACG
GAATGCAGACACTACAAGAATCAGGCATTGAAAAGCTCAAAAACGGCGTAACCAGCTTCAAAGAGCTGCAACGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

83.274

100

0.833
  pilB Vibrio campbellii strain DS40M4

74.555

100

0.746
  pilB Vibrio parahaemolyticus RIMD 2210633

73.132

100

0.731
  pilB Acinetobacter baumannii D1279779

52.115

92.527

0.482
  pilB Legionella pneumophila strain ERS1305867

51.758

91.103

0.472
  pilB Acinetobacter baylyi ADP1

50.19

93.594

0.47
  pilF Neisseria gonorrhoeae MS11

44.248

100

0.445
  pilF Thermus thermophilus HB27

37.589

100

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

35.932

100

0.377