Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L1871_RS19730 Genome accession   NZ_CP091176
Coordinates   4181773..4183479 (+) Length   568 a.a.
NCBI ID   WP_104454847.1    Uniprot ID   -
Organism   Aeromonas caviae strain SCLZS52     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4176773..4188479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1871_RS19705 (L1871_19700) pdhR 4177930..4178694 (-) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  L1871_RS19710 (L1871_19705) ampD 4178971..4179543 (-) 573 WP_039040507.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L1871_RS19715 (L1871_19710) - 4179672..4180142 (+) 471 WP_042015101.1 TIGR02281 family clan AA aspartic protease -
  L1871_RS19720 (L1871_19715) nadC 4180146..4181009 (+) 864 WP_052814467.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L1871_RS19725 (L1871_19720) tapA 4181332..4181769 (+) 438 WP_104456018.1 type IVa pilus major pilin TapA -
  L1871_RS19730 (L1871_19725) pilB 4181773..4183479 (+) 1707 WP_104454847.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  L1871_RS19735 (L1871_19730) pilC 4183607..4184848 (+) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  L1871_RS19740 (L1871_19735) pilD 4184926..4185801 (+) 876 WP_103261555.1 prepilin peptidase Machinery gene
  L1871_RS19745 (L1871_19740) coaE 4185820..4186434 (+) 615 WP_052814464.1 dephospho-CoA kinase -
  L1871_RS19750 (L1871_19745) zapD 4186472..4187194 (+) 723 WP_010675825.1 cell division protein ZapD -
  L1871_RS19755 (L1871_19750) yacG 4187205..4187399 (+) 195 WP_103858092.1 DNA gyrase inhibitor YacG -
  L1871_RS19760 (L1871_19755) mutT 4187457..4187861 (-) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62550.90 Da        Isoelectric Point: 5.8214

>NTDB_id=648196 L1871_RS19730 WP_104454847.1 4181773..4183479(+) (pilB) [Aeromonas caviae strain SCLZS52]
MTSSPNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=648196 L1871_RS19730 WP_104454847.1 4181773..4183479(+) (pilB) [Aeromonas caviae strain SCLZS52]
ATGACCTCAAGTCCCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCAAAGCCCTCG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTCGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGTTTCAGTTTTGGCCTGCACACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCAGACGATGATGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGATATTGCCGAACGGCGCCTGCCCCAGGATGGCCGGATCAAACTCAAGATCTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGATTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAACTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCTACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCTGAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTTGAGATAAACCTGCCCGGTGTCAACCAGGTGCAGATCAATCCCAAAG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAAAAAGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.737

100

0.549
  pilB Acinetobacter baumannii D1279779

55.026

99.824

0.549
  pilB Legionella pneumophila strain ERS1305867

54.867

99.472

0.546
  pilB Vibrio cholerae strain A1552

53.415

100

0.537
  pilB Vibrio campbellii strain DS40M4

50.53

99.648

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.178

98.944

0.496
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37