Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L1O00_RS01920 Genome accession   NZ_CP091140
Coordinates   400609..402315 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain b1-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 395609..407315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1O00_RS01890 (L1O00_01890) mutT 396119..396523 (+) 405 WP_278171360.1 8-oxo-dGTP diphosphatase MutT -
  L1O00_RS01895 (L1O00_01895) yacG 396601..396795 (-) 195 WP_069554211.1 DNA gyrase inhibitor YacG -
  L1O00_RS01900 (L1O00_01900) zapD 396805..397527 (-) 723 WP_278171363.1 cell division protein ZapD -
  L1O00_RS01905 (L1O00_01905) coaE 397565..398179 (-) 615 WP_278171366.1 dephospho-CoA kinase -
  L1O00_RS01910 (L1O00_01910) pilD 398193..399065 (-) 873 WP_278171368.1 A24 family peptidase Machinery gene
  L1O00_RS01915 (L1O00_01915) pilC 399127..400368 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  L1O00_RS01920 (L1O00_01920) pilB 400609..402315 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  L1O00_RS01925 - 402315..402737 (-) 423 WP_106843508.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  L1O00_RS01930 (L1O00_01930) nadC 403053..403916 (-) 864 WP_278171372.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L1O00_RS01935 (L1O00_01935) - 403920..404390 (-) 471 WP_278171374.1 retropepsin-like aspartic protease -
  L1O00_RS01940 (L1O00_01940) ampD 404514..405083 (+) 570 WP_278171376.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L1O00_RS01945 (L1O00_01945) pdhR 405362..406126 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=647967 L1O00_RS01920 WP_005336134.1 400609..402315(-) (pilB) [Aeromonas veronii strain b1-3]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=647967 L1O00_RS01920 WP_005336134.1 400609..402315(-) (pilB) [Aeromonas veronii strain b1-3]
ATGACCTCTAGTCCTAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCACTCATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTCACATTTCTGATAGAGAACGAAATCCTCGACAGTAAAGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATTCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTGCTGCCCATCTACACTCAGGGCCACACCCTATACATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGGCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAATTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCGGATCTCGAGGTCTCCGACGAAAACAGTCGGCTGGACGAGAGCGTCAATACTGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGACGCCATCAAGCGTGGTGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTCCGCTTTCGCATCGATGGCATCCTGCACGAGATCGCCACACCGCCGGTCAATCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTGATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGACTCACCTTTGCCAGCGCCTTGCGCTCCTTCTTGCGGCAAGATCCGGATGTTGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAGATCGCTATCAAGGCGGCGCAGACCGGTCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGTTGGCAGCCGGACTGCGACTGTTCAAACCAGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAAGGGGCCAACTCCCTGCAAATCGCCG
CGATCGCCCAGAAGGAAGGGATGCGTACTCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACAGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377