Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L1S45_RS02585 Genome accession   NZ_CP091091
Coordinates   542729..544435 (-) Length   568 a.a.
NCBI ID   WP_065017268.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain b2-100     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 537729..549435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1S45_RS02555 (L1S45_02555) mutT 538405..538818 (+) 414 WP_026141640.1 8-oxo-dGTP diphosphatase MutT -
  L1S45_RS02560 (L1S45_02560) yacG 538879..539073 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  L1S45_RS02565 (L1S45_02565) zapD 539083..539805 (-) 723 WP_025202564.1 cell division protein ZapD -
  L1S45_RS02570 (L1S45_02570) coaE 539843..540457 (-) 615 WP_252603446.1 dephospho-CoA kinase -
  L1S45_RS02575 (L1S45_02575) pilD 540477..541349 (-) 873 WP_024943109.1 A24 family peptidase Machinery gene
  L1S45_RS02580 (L1S45_02580) pilC 541372..542613 (-) 1242 WP_005305649.1 type II secretion system F family protein Machinery gene
  L1S45_RS02585 (L1S45_02585) pilB 542729..544435 (-) 1707 WP_065017268.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  L1S45_RS02590 (L1S45_02590) tapA 544439..544867 (-) 429 WP_252603448.1 type IVa pilus major pilin TapA -
  L1S45_RS02595 (L1S45_02595) nadC 545189..546046 (-) 858 WP_252603451.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L1S45_RS02600 (L1S45_02600) - 546052..546522 (-) 471 WP_043171614.1 retropepsin-like aspartic protease -
  L1S45_RS02605 (L1S45_02605) ampD 546646..547218 (+) 573 WP_017784976.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L1S45_RS02610 (L1S45_02610) pdhR 547494..548258 (+) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62556.96 Da        Isoelectric Point: 5.4363

>NTDB_id=647594 L1S45_RS02585 WP_065017268.1 542729..544435(-) (pilB) [Aeromonas dhakensis strain b2-100]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMENDQDALGMEEIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=647594 L1S45_RS02585 WP_065017268.1 542729..544435(-) (pilB) [Aeromonas dhakensis strain b2-100]
ATGACCTCTAGTCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAACCCTTTGTGACCTTTCTGATCGAGAACGAGATCCTCGACAGCAAGGCGCTGG
CCGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTACTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCCGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGCTGCACACCGAGGCCCTGCTGGTCGAAGAGAACA
AGCTGACCGCCGCCATCAGCAAGCTGATGGAGAACGATCAGGACGCCCTCGGCATGGAGGAGATCGACGAATCCGAGATC
TCCGAGCTGGAGGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCTCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGACCAAGTACC
GTATTCGTTTTCGGATAGACGGGATCCTGCATGAGGTAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCGCGCCTAGACATTGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAAATGTCGCGCAA
CAAGTCGATGGACATGCGGGTCAACACCCTGCCTACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTCGACTCTTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGCTTTGACGATCGGCAGAAGGCGCAATATCTGCGCGCGCTCTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATTCTCAACACCAC
AGAAGTGAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCTGGGGTCAATCAGGTGCAGGTCAACCCCAAGG
CTGGACTGACCTTCGCCAGTGCCCTGCGCTCTTTCCTGCGCCAGGATCCCGATGTGGTGATGGTCGGGGAAATACGGGAT
CTTGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTTTCCACATTGCACACCAACTCGGCGGC
CGAGACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCATGGCTCAGC
GACTGGCACGCAAGCTCTGTGACCACTGCAAGGCTCCCGAAGTGGTGCCGGAAGCTGAACTGCTGGAGTTGGGGTTCACC
CAACAGCAGCTGGCCGCAGGCTTGCGGCTGTTCAAGCCGGTCGGCTGCAAGGAGTGTTCGGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCCTGTTGATGTCCGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGCAGATTGCGG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGGACCTCCGGTCTTGAGAAGGCCCGACTGGGGGTGACCAGCCTGGCC
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Acinetobacter baumannii D1279779

55.088

100

0.553
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Vibrio cholerae strain A1552

53.982

99.472

0.537
  pilB Vibrio campbellii strain DS40M4

51.767

99.648

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.068

98.944

0.505
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

40.53

92.958

0.377