Detailed information NTG0646766

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L1O99_RS11600
• Genome accession: NZ_CP090971
• Coordinates: 2677012..2678142 (-)
• Length: 376 a.a.
• NCBI ID: WP_012916642.1
• Uniprot ID: D2UEP6
• Organism: Xanthomonas albilineans strain Xa21
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2672012..2683142

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L1O99_RS11580	-	2674096..2675043 (-)	948	WP_012916638.1	aspartate carbamoyltransferase catalytic subunit	-
L1O99_RS11585	ruvX	2675062..2675559 (-)	498	WP_012916639.1	Holliday junction resolvase RuvX	-
L1O99_RS11590	-	2675552..2676118 (-)	567	WP_012916640.1	YqgE/AlgH family protein	-
L1O99_RS11595	-	2676434..2676973 (+)	540	WP_012916641.1	DNA-3-methyladenine glycosylase I	-
L1O99_RS11600	pilU	2677012..2678142 (-)	1131	WP_012916642.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
L1O99_RS11605	pilT	2678267..2679304 (-)	1038	WP_012916643.1	type IV pilus twitching motility protein PilT	Machinery gene
L1O99_RS11610	-	2679407..2680081 (+)	675	WP_080928215.1	YggS family pyridoxal phosphate-dependent enzyme	-
L1O99_RS11615	-	2680347..2681387 (+)	1041	WP_230598079.1	hypothetical protein	-
L1O99_RS11620	proC	2681537..2682379 (+)	843	WP_012916646.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 41941.21 Da
• Isoelectric Point: 6.8813

>NTDB_id=646766 L1O99_RS11600 WP_012916642.1 2677012..2678142(-) (pilU) [Xanthomonas albilineans strain Xa21]
MSTTDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTPQQSRDMVLNVMTPGQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPSVDELSLPPVIKTLAMTKRGIIIFVGATGAGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMELSLNLRGVVAQQLIPTPDGKSRRVAMEIMLGTPLIQDYIREGEIHKLKDVMKESINLGMRTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide

• Length: 1131 bp

>NTDB_id=646766 L1O99_RS11600 WP_012916642.1 2677012..2678142(-) (pilU) [Xanthomonas albilineans strain Xa21]
ATGAGTACCACCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAAAAAGCGTCGGATCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACATGG
TGTTGAACGTGATGACGCCTGGGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTTGGCCGCTTCCGCGTCAGTTGCTTCTACCAGCGTAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATTCC
CAGCGTGGACGAACTGAGCTTGCCGCCGGTGATTAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTTGGTG
CGACCGGGGCCGGTAAATCGACCTCACTGGCGGCGATGATCGGTTATCGCAACCAGAACTCCACCGGACACATCATCACC
ATCGAAGATCCGATTGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGGATCGACACCGACAG
CTGGGAAAACGCATTGAAAAATACCCTGCGTCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATTTCCTTCGCCGAAACCGGCCACCTGGTGCTGTGTACTTTGCATGCCAACAACGCCAACCAGGCGATG
GATCGCGTCATCAACTTCTTCCCTGAAGACCGTCGCAGTCAATTGTTGATGGAACTATCGCTGAACCTGCGGGGCGTGGT
CGCCCAGCAATTGATCCCGACTCCGGATGGCAAGAGCCGTCGGGTGGCGATGGAAATCATGCTCGGGACTCCGTTGATCC
AGGATTACATCCGCGAAGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAGTCCATCAATCTGGGCATGCGAACCTTC
GATCAGAGCTTGTTCGAGTTGTATCAGGCTGGCGAGATCAGCTACGAGGACGCGCTGCGTCACGCCGATTCGCAGAACGA
AGTGCGCTTGCGCATCAAGTTGGCCCAGGGCGGCGACGCCAGAACCCTGGCGCAGGGCTTGGACGGGGTCGAGATCGCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-272)

3D structure

• AlphaFold DB: D2UEP6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	65.574	97.34	0.638
pilU	Acinetobacter baylyi ADP1	62.921	94.681	0.596
pilU	Vibrio cholerae strain A1552	52.394	94.415	0.495
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.857	93.085	0.38
pilT	Acinetobacter baumannii strain A118	40.233	91.223	0.367
pilT	Acinetobacter nosocomialis M2	40.233	91.223	0.367
pilT	Acinetobacter baumannii D1279779	40.233	91.223	0.367
pilT	Pseudomonas aeruginosa PAK	40.828	89.894	0.367