Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L1B16_RS14730 Genome accession   NZ_CP090954
Coordinates   3414697..3415827 (-) Length   376 a.a.
NCBI ID   WP_016902355.1    Uniprot ID   -
Organism   Xanthomonas arboricola pv. pruni strain Xcp1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3409697..3420827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1B16_RS14700 (L1B16_03345) - 3410079..3410519 (-) 441 WP_016902361.1 OsmC family protein -
  L1B16_RS14705 (L1B16_03350) - 3410730..3411677 (-) 948 WP_039513835.1 aspartate carbamoyltransferase catalytic subunit -
  L1B16_RS14710 (L1B16_03355) ruvX 3411693..3412160 (-) 468 WP_039513833.1 Holliday junction resolvase RuvX -
  L1B16_RS14715 (L1B16_03360) - 3412153..3412719 (-) 567 WP_039513830.1 YqgE/AlgH family protein -
  L1B16_RS14720 (L1B16_03365) - 3413054..3413599 (+) 546 WP_039813256.1 DNA-3-methyladenine glycosylase I -
  L1B16_RS14725 (L1B16_03370) - 3413737..3414411 (-) 675 WP_024940756.1 YitT family protein -
  L1B16_RS14730 (L1B16_03375) pilU 3414697..3415827 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L1B16_RS14735 (L1B16_03380) pilT 3415950..3416987 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  L1B16_RS14740 (L1B16_03385) - 3417310..3418002 (+) 693 WP_039513823.1 YggS family pyridoxal phosphate-dependent enzyme -
  L1B16_RS14745 (L1B16_03390) proC 3418064..3418909 (+) 846 WP_039813263.1 pyrroline-5-carboxylate reductase -
  L1B16_RS14750 (L1B16_03395) - 3419507..3419932 (+) 426 WP_026064288.1 HU family DNA-binding protein -
  L1B16_RS14755 (L1B16_03400) - 3420068..3420688 (+) 621 WP_039513819.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41966.24 Da        Isoelectric Point: 6.8287

>NTDB_id=646624 L1B16_RS14730 WP_016902355.1 3414697..3415827(-) (pilU) [Xanthomonas arboricola pv. pruni strain Xcp1]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=646624 L1B16_RS14730 WP_016902355.1 3414697..3415827(-) (pilU) [Xanthomonas arboricola pv. pruni strain Xcp1]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGTCCGATCACCCAGACTCCGCTGACCGCGCAACAGAGCCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAATTCGAAAAAACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGCTTTCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGTATCCC
CACGGTGGAAGAGTTGAGCCTTCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGGCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGATCATGCCGTGGCCTTCGCCGAAACCGGCCATCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAACCAGTTGCTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGTTGATTCCGACCCCGGATGGCCGCGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACACCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGTCGCAGGGCGGCGACGCCAAGACGCTGGCGCAGGGCCTGGATGGCGTGGAGATCGCCG
AGGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367