Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L0U97_RS00200 Genome accession   NZ_CP090890
Coordinates   39776..40951 (+) Length   391 a.a.
NCBI ID   WP_004914541.1    Uniprot ID   S7Y0P3
Organism   Acinetobacter junii strain WCO-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 34776..45951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0U97_RS00175 - 35440..35913 (-) 474 WP_004914520.1 hypothetical protein -
  L0U97_RS00180 guaD 36099..37424 (+) 1326 WP_234856672.1 guanine deaminase -
  L0U97_RS00185 hpt 37641..38168 (+) 528 WP_234856673.1 hypoxanthine phosphoribosyltransferase -
  L0U97_RS00190 - 38397..38648 (+) 252 WP_004949988.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  L0U97_RS00195 - 38698..39396 (-) 699 WP_234856674.1 DsbC family protein -
  L0U97_RS00200 pilU 39776..40951 (+) 1176 WP_004914541.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L0U97_RS00205 mnmE 41007..42362 (-) 1356 WP_035338451.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  L0U97_RS00210 yidC 42453..44210 (-) 1758 WP_234856675.1 membrane protein insertase YidC -
  L0U97_RS00215 yidD 44216..44536 (-) 321 WP_004950001.1 membrane protein insertion efficiency factor YidD -
  L0U97_RS00220 rnpA 44546..44941 (-) 396 WP_004950005.1 ribonuclease P protein component -
  L0U97_RS00225 rpmH 44970..45104 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44323.84 Da        Isoelectric Point: 6.3220

>NTDB_id=646177 L0U97_RS00200 WP_004914541.1 39776..40951(+) (pilU) [Acinetobacter junii strain WCO-9]
MYTTELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTKLFAYSLMNDKQRLDFENDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAIEILLNTPLIADNILKGQFHVLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSSRQDGMPVSTTSLNMVKEEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=646177 L0U97_RS00200 WP_004914541.1 39776..40951(+) (pilU) [Acinetobacter junii strain WCO-9]
ATGTATACCACTGAATTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTCACTAAAGTAGTTGAGTATGGGGGATC
TGATTTATTTATTACCGCAGATTTCCCACCGAGCATCAAGTTACAGGGTTTGATGCGCCCTTTAGGACAACAAGCGCTTA
CGGCAGAAAAAACCAAGTTGTTTGCTTATAGTTTAATGAATGACAAACAACGTCTAGACTTCGAAAATGATTGGGAATGT
AACTTTGCCATTAATGTCCCGAATGTTTCACGGTTTCGGGTCAATGTATTTAAACAACAATTACAAATAGGCATGGTGAT
TCGAACCATTACGTCTGAAATTCCAACCTTTCAAAAACTAAAACTCCCAGAATCACTAAAAAATGTGATTATGGAAAAAC
GAGGTTTGGTACTGGTGGTTGGCGGAACAGGATCAGGAAAATCAACCTCTTTAGCAGCCATGATTGATCATCGAAATGAG
AACTCTGCTGGACATATCATTACCGTTGAAGATCCTGTTGAATACGTCCATAAACACAAAAAATCCATGATCACGCACCG
TGAGGTTGGAGTTGATTGTCATACATGGCATCACGCGTTGAAAAATACGCTACGCCAAGCACCAGATGTGATTTTAATTG
GTGAAATACGTGATACTGAAACGATGGAACATGCGATTGCTTTTGCTGAAACAGGGCATCTATGTTTGGGAACACTGCAC
GCCAACAATGCCAATCAGGCACTGGATCGTATTATTAACTTTTTCCCAGAGGAACGTCGCAATCAACTGTTGATGGATCT
CTCATCCAATATGAAAGCGATTATTTCTCAACGTCTCGTACGAACTCAAGATGGTCAAGGACGTCGAGCTGCCATTGAAA
TCCTCCTCAATACGCCATTGATCGCAGACAATATTTTGAAAGGGCAATTCCATGTGCTCAAAGAGATCATGACCAAATCT
CGTGAGTTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATCTGTATAACGAAGGTGCAATTAGTTACGATGAAGCATT
GCGTAACGCCGACTCTGTCAATGAATTACGCTTGCAAATTAAATTAAAAAGTTCACGCCAAGATGGGATGCCCGTTTCAA
CCACTTCACTGAACATGGTCAAAGAAGAGGAAGTTGAAGAGCAAGAAGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7Y0P3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.197

93.606

0.545
  pilU Acinetobacter baylyi ADP1

55.62

88.747

0.494
  pilU Vibrio cholerae strain A1552

52.601

88.491

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

85.678

0.371
  pilT Acinetobacter nosocomialis M2

44.172

83.376

0.368
  pilT Acinetobacter baumannii D1279779

44.172

83.376

0.368
  pilT Acinetobacter baumannii strain A118

44.172

83.376

0.368
  pilT Acinetobacter baylyi ADP1

42.56

85.934

0.366